2010 Fiscal Year Final Research Report
Gene regulatory network involved in the formation of central nervous system in ascidian embryos
| Project/Area Number |
20247031
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto University |
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Principal Investigator |
SATOH Noriyuki Kyoto University, マリンゲノミックスユニット, 代表研究者 (30025481)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAMURA Katsumi 福山大学, 生命工学部, 准教授 (30206883)
SASAKURA Yasunori 筑波大学, 大学院・生命環境科学研究科, 講師 (10400649)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 脊索動物 / ホヤ / 中枢神経系 / 発生と分化 / 遺伝子発現 / 遺伝子ネットワーク / トランスジェニック系 / マイクロアレイ |
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| Research Abstract |
Despite containing only approximately 350 cells, the central nervous system (CNS) of Ciona intestinalis larvae has an architecture that is similar to the vertebrate CNS. To identify genes expressed in the CNS, we isolated CNS cells using transgenic embryos that showed robust Kaede expression in the larval CNS. With the aid of microarray, we identified 565 genes that are preferentially expressed in the CNS. In addition, tracing cells with specific gene expression and functional analyses of the genes resulted in several discoveries of the gene regulatory network involved in the formation of the Ciona larval CNS.
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[Journal Article] Ependymal cells of chordate larvae arestem-like cells that form the adult nervoussystem.2011
Author(s)
Horie, T., Shinki, R., Ogura, Y., Kusakabe, T. G., Satoh, N., Sasakura, Y.
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Journal Title
Nature 469
Pages: 525-528
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[Journal Article] Expression of neuropeptide- and hormone-encoding genes in the Ciona intestinalis larval brain.2011
Author(s)
Hamada, M., Shimozono, N., Ohta, N., Satou, Y., Horie, T., Kawada, T., Satake, H., Sasakura, Y., Satoh, N.
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Journal Title
Dev Biol 352
Pages: 202-214
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[Journal Article] Coordination of mitosis and morphogenesis: role of a prolonged G2 phase during chordate neurulation.2011
Author(s)
Ogura, Y., Sakaue-Sawano, A., Nakagawa, M., Satoh, N., Miyawaki, A., Sasakura, Y.
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Journal Title
Development 138
Pages: 577-587
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[Journal Article] Efficient transposition of a single Minos transposon copy in the genome of the ascidian Ciona intestinalis with a transgenic line expressing transposase in eggs.2010
Author(s)
Hozumi, A., Kawai, N., Yoshida, R., Ogura, Y., Ohta, N., Satake, H., Satoh, N., Sasakura, Y.
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Journal Title
Dev Dyn 239
Pages: 1076-1088
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[Journal Article] Network structure of projections extending from peripheral neurons in the tunic of ascidian larva.2010
Author(s)
Terakubo, H.Q., Nakajima, Y., Sasakura, Y., Horie, T., Konno, A., Takahashi, H., Inaba, K., Hotta, K., Oka, K.
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Journal Title
Dev Dyn 239
Pages: 2278-2287
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[Presentation] Ependymal cells of chordate larvae are stem-like cells that form the adult nervous system.2010
Author(s)
Horie, T., Shinki, R., Ogura, Y., Kusakabe, T.G., Satoh, N., Sasakura, Y.
Organizer
The 33^<rd> Annual Meeting of Molecular Biology of Japan
Place of Presentation
Kobe, Japan.
Year and Date
20101207-20101210
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