Cloning of avirulence genes in Pyricularia spp. using super SAGE and comparative analysis of their instability

2011 Fiscal Year Final Research Report

• Project/Area Number: 20248005
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kobe University
• Principal Investigator: TOSA Yukio 神戸大学, 大学院・農学研究科, 教授 (20172158)
• Co-Investigator(Kenkyū-buntansha): TERAUCHI Ryohei 岩手生物工学研究センター, 主席研究員 (50236981) ; KUSABA Motoaki 佐賀大学, 農学部, 准教授 (90304881)
• Project Period (FY): 2008 – 2011
• Keywords: いもち病 / 非病原力遺伝子

Research Abstract

We found that AVR-Pita, AVR-Pia, AVR-Pii, AVR-Pik, avirulence genes of the rice blast fungus, are highly variable in its genome location, occurring in chromosomes 1 through 7 and supernumerary chromosomes. They were flanked by retrotransposons, which presumably contributed to their "multiple translocation" across the genome during the course of evolution in response to corresponding resistance genes. We also found an avirulence gene that has not undergone multiple translocation. We suggest that the stability of avirulence genes is predictable on the basis of the their chromosomal locations

Research Products

• [Journal Article] Instability of subtelomeric regions during meiosis in Magnaporthe oryzae (2011)
  • Author(s): Chuma, I., Hotta, Y., and Tosa, Y.
  • Journal Title: J. Gen. Plant Pathol Volume: 77 Pages: 317-325
• [Journal Article] Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species (2011)
  • Author(s): Chuma, I., Isobe, C., Hotta, Y., Ibaragi, K., Futamata, N., Kusaba, M., Yoshida, K., Terauchi, R., Fujita, Y., Nakayashiki, H., Valent, B., and Tosa, Y.
  • Journal Title: PLoS Pathogens Pages: e1002147
• [Journal Article] PWT1, an avirulence gene of Magnaporthe oryzae tightly linked to the rDNA locus, is recognized by two staple crops (2010)
  • Author(s): Chuma, I., Zhan, S. W., Asano, S., Nga, N. T. T., Vy, T. T. P., Shirai, M., Ibaragi, K., and Tosa, Y.
  • Journal Title: common wheat and barley. Phytopathology Volume: 100 Pages: 436-443
• [Journal Article] Association genetics reveals three novel avirulence genes from the rice blast fungal pathogen Magnaporthe oryzae (2009)
  • Author(s): Yoshida, K., Saitoh, H., Fujisawa, S., Kanzaki, H., Matsumura, H., Yoshida, K., Tosa, Y., Chuma, I., Takano, Y., Win, J., Kamoun, S., and Terauchi, R.
  • Journal Title: Plant Cell Volume: 21 Pages: 1573-1591
• [Journal Article] An avirulence gene to rice cultivar K60 is located on the 1. 6-Mb chromosome in Magnaporthe oryzae isolate 84R-62B (2008)
  • Author(s): Kusaba, M., Luo, C. X., Hanamura, H., Misaka, M., Mochida, T., Fujita, Y., and Tosa, Y.
  • Journal Title: J. Gen. Plant Pathol Volume: 74 Pages: 250-253
• [Presentation] Roles of avirulence genes o Pyricularia species in defining host and nonhost specificity (2011)
  • Author(s): Tosa, Y.
  • Organizer: Plant and Animal Genome XIX
  • Place of Presentation: San Diego, CA. USA
  • Year and Date: 20110000
• [Presentation] Genetic analyses of host species specificity of Mangaporthe oryzae subgroups (2010)
  • Author(s): Tosa, Y. and Chuma I.
  • Organizer: 10^<th> Japan-US Seminar
  • Place of Presentation: Oregon, USA
  • Year and Date: 20100000
• [Book] Searching for effectors of Magnaporthe oryzae : a multi-faceted genomics approach (2009)
  • Author(s): Terauchi, R., Win, J., Kamoun, S., Matsumura, H., Saitoh, H., Kanzaki, H., Yoshida, K., Shenton, M., Berberich, T., Fujisawa, S., Ito, A., Takano, Y., and Tosa, Y.
  • Total Pages: 105-112
  • Publisher: Springer
• [Book] Biology of Molecular Plant-Microbe Interactions (2008)
  • Author(s): Terauchi, R., Win, J., Kamoun, S., Matsumura, H., Saitoh, H., Yoshida, K., Shenton, M., Berberich, T., Fujisawa, S., Ito, A., Takano, Y., and Tosa, Y.
  • Total Pages: 114