Anti-insect defense proteins and chemicals in plant latex and their molecular modes of action

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20248007
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: KONNO Kotaro National Institute of Agrobiological Sciences, 昆虫-昆虫・植物間相互作用研究ユニット, 主任研究員 (00355744)
• Project Period (FY): 2008 – 2011
• Keywords: 植物乳液 / 滲出液 / 耐虫性2次代謝物質 / 耐虫防御タンパク質 / 植物被食防衛

Research Abstract

本研究計画では、農業上必要な耐虫性植物育種の素材として種々の植物が傷口からだす白色の乳液やそれに類似した滲出液が含む耐虫性タンパク質・物質を特定しその耐虫性発現機構の解明を行う。特に乳液を持つ代表的な植物で種類も多いクワ科植物各種(イチジク属・クワ属)の乳液に含まれる耐虫性因子(2次代謝物質・タンパク質)の同定を目指す。中でもクワ乳液に関しては、乳液に含まれる新規耐虫性タンパク質MLX56に関してその耐虫性メカニズムの解明を目指す。さらに、他の植物の乳液や乳液に類似の滲出液(ウリ科植物篩管滲出液等)に関して耐虫活性の検定と耐虫性物質の同定を行う。

Research Products

• [Journal Article] Plant latex and other exudates as plant defense systems : Roles of various defense chemicals and proteins contained therein. (2011)
  • Author(s): Kotaro Konno
  • Journal Title: Phytochemistry (Special Issue on Plant-Insect Interactions) Pages: 10.1016/j.phytochem,21
  • Peer Reviewed
• [Journal Article] A unique latex protein, MLX56, defends mulberry trees from insects. (2009)
  • Author(s): Naoya Wasano, Kotaro Konno, Masatoshi Nakamura, Chikara Hirayama, Makoto Hattori, Ken Tateishi
  • Journal Title: Phytochemistry 70 Pages: 880-888
  • Peer Reviewed
• [Journal Article] Latex : A model for understanding mechanisms, ecology and evolution of plant defense against herbivory. (2009)
  • Author(s): Anurag A.Agrawal, Kotaro Konno
  • Journal Title: The Annual Review of Ecology, Evolution, and Systematics 40 Pages: 311-331
  • Peer Reviewed
• [Presentation] Purification of insect-resistant proteins from the wax gourd, Benincasa hispida. (2010)
  • Author(s): Eiji Ota, Masatoshi Nakamura, Chikara Hirayama, Kotaro Konno
  • Organizer: International Society of Chemical Ecology 26^<th> Annual Meeting
  • Place of Presentation: Tours, France.
  • Year and Date: 20100802-20100804
• [Presentation] Phenanthroindolizidine alkaloids : Defense chemicals found in the latex of Ficus septica. (2010)
  • Author(s): Kotaro Konno, Masatoshi Nakamura, Naoya Wasano, Eiji Ota, Chikara Hirayama, Ken Tateishi, Khondkar E.Kabir, Makiko Takenaka, Hiroshi Ono, Keiichi Honda
  • Organizer: International Society of Chemical Ecology 26^<th> Annual Meeting
  • Place of Presentation: Tours, France.
  • Year and Date: 20100802-20100804
• [Presentation] Interspecific diversity of latex ingredients among Moraceae plants : Defense chemicals and proteins playing important roles in plant-herbivorous interactions. (2010)
  • Author(s): Kotaro Konno, Naoya Wasano, Masatoshi Nakamura, Ken Tateishi, Cikara Hirayama, Makiko Takenaka, Hiroshi Ono
  • Organizer: Gordon Research Conference on Plant- Herbivore Interaction
  • Place of Presentation: Galveston, Texas, USA.
  • Year and Date: 2010-02-24
• [Book] In "Comprehensive Natural Products II Chemistry and Biology" (Mander, L., Lui, H.-W., Eds.) (2010)
  • Author(s): Hisakazu Yamane, Kotaro Konno, Maurice Sabelis, Junji Takabayashi, Tekeshi Sassa, Hideaki Oikawa
  • Total Pages: 339-385
  • Publisher: Elsevier, Oxford, Section 4.08 "Chemical Defence and Toxins of Plants"
• [Patent(Industrial Property Rights)] 耐虫性タンパク質及び該耐虫性タンパク質をコードする耐虫性遺伝子 (2009)
  • Inventor(s): 今野浩太郎・和佐野直也
  • Industrial Property Rights Holder: 今野浩太郎・農業生物資源研究所(アメリカ以外)、今野浩太郎、和佐野直也(アメリカ)
  • Industrial Property Number: 特許,PCT/JP2008/53794
  • Filing Date: 20090000