• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2011 Fiscal Year Final Research Report

Elucidation of novel nitrogen metabolisms of microbes

Research Project

  • PDF
Project/Area Number 20248009
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionThe University of Tokyo

Principal Investigator

SHOUN Hirofumi  東京大学, 大学院・農学生命科学研究科, 名誉教授 (70012036)

Co-Investigator(Kenkyū-buntansha) SASAKI Yasuyuki  東京農業大学, 応用生物科学部, 助教 (50398814)
FUSHINOBU Shinya  東京大学, 大学院・農学生命科学研究科, 教授 (00302589)
WAKAGI Takayoshi  東京大学, 大学院・農学生命科学研究科, 研究員 (70175058)
Project Period (FY) 2008 – 2011
Keywords微生物代謝 / 窒素代謝
Research Abstract

We studied novel nitrogen metabolisms of Eukaryotic microbes (fungi) and Bacteria (Actinomycetes and denitrifier). For the fungal denitrification system, we succeeded in obtaining the gene for nitrite reductase (nirk), providing a novel point of view about the origin and evolution of mitochondria. In addition, we studied fungal codenitrification, a novel nitrogen metabolism of S. coelicolor, and the characteristics and survival of P. stutzeri TR2.

  • Research Products

    (16 results)

All 2012 2011 2010 2009

All Journal Article (11 results) Presentation (5 results)

  • [Journal Article] anitrite-denitrifying bacterium isolated from nitrifying-denitrifying activated sludge collected from a laboratory-scale bioreactor treating piggery wastewater2012

    • Author(s)
      M. Matsuoka, S. Park, S.-Y. An, M.Miyahara, S.-W. Kim, K. Kamino, S. Fushinobu, A. Yokota, T. Wakagi, and H. Shoun, Advenella faeciporci sp. nov.
    • Journal Title

      Int. J. Syst. Evol. Microbiol

      Volume: 62 Pages: 2986-2990

    • DOI

      doi:10.1099/ijs.0.037440-0

  • [Journal Article] Survival of the aerobic denitrifier Pseudomonas stutzeri Strain TR2 during co-culture with activated sludge under denitrifying conditions2012

    • Author(s)
      M. Miyahara, S.-W. Kim, S. Zhou, S.Fushinobu, T. Yamada, W. Ikeda-Ohtsubo, A. Watanabe, K. Miyauchi, G. Endo, T. Wakagi, and H. Shoun
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 76 Pages: 495-500

    • DOI

      doi:10.1271/bbb.110785

  • [Journal Article] Fungal denitrification and nitric oxide reductase cytochrome P450nor2012

    • Author(s)
      H. Shoun, S. Fushinobu, L. Jiang, S.-W.Kim, and T. Wakagi
    • Journal Title

      Phil. Trans. R. Soc. B

      Volume: 367 Pages: 1186-1194

    • DOI

      doi:10.1098/rstb.2011.0335

  • [Journal Article] Functional analysis and subcellular location of two flavohemoglobins from Aspergillus orizae2011

    • Author(s)
      S. Zhou, S. Fushinobu, S.-W. Kim, Y.Nakanishi, J.-I. Maruyama, K. Kitamoto, T. Wakagi, and H. Shoun
    • Journal Title

      Fungal Genet. Biol

      Volume: 48 Pages: 200-207

    • DOI

      doi:10.1016/j.fgb.2010.08.011

  • [Journal Article] The possible involvement of copper-containingnitrite reductase (NirK) and flavohemoglobin in denitrification by the fungus Cylindrocarpon tonkinense2010

    • Author(s)
      S.-W. Kim, S. Fushinobu, S. Zhou, T. Wakagi, and H. Shoun
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 74 Pages: 1403-1407

    • DOI

      doi:10.1271/bbb.100071

  • [Journal Article] Potential of aerobic denitrification by Pseudomonas stutzeri TR2 to reduce nitrous oxide emissions from wastewater treatment plants2010

    • Author(s)
      M. Miyahara, S.-W. Kim, S. Fushinobu, K.Takaki, T. Yamada, A. Watanabe, K. Miyauchi, G. Endo, T. Wakagi, and H. Shoun
    • Journal Title

      Appl. Environ. Microbiol

      Volume: 76 Pages: 4619-4625

    • DOI

      doi:10.1128/AEM.01983-09

  • [Journal Article] Aspergillus oryzae flavohemoglobins promote oxidative damage by hydrogen peroxide2010

    • Author(s)
      S. Zhou, S. Fushinobu, S.-W. Kim, Y.Nakanishi, T. Wakagi, and H. Shoun
    • Journal Title

      Biochem. Biophys. Res. Comm

      Volume: 394 Pages: 558-561

    • DOI

      doi:10.1016/j.bbrc.2010.03.018

  • [Journal Article] A eukaryotic copper-containing nitrite reductase derived from a NirK homolog gene of Aspergillus oryzae2010

    • Author(s)
      Y. Nakanishi, S. Zhou, S.-W. Kim, S. Fushinobu, J. Maruyama, K. Kitamoto, T. Wakagi, and H. Shoun
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 74 Pages: 984-991

    • DOI

      doi:10.1271/bbb.90844

  • [Journal Article] Nitrous oxide emission from nitrifying activated sludge dependent on denitrification by ammonia-oxidizing bacteria2010

    • Author(s)
      S.-W. Kim, M. Miyahara, S. Fushinobu, T.Wakagi, and H. Shoun
    • Journal Title

      Bioresour. Technol

      Volume: 101 Pages: 3958-3963

    • DOI

      doi:10.1016/j.biortech.2010.01.030

  • [Journal Article] Eukaryotic nirK genes encoding copper-containing nitrite reductase: originating from theprotomitochondrion?2009

    • Author(s)
      S.-W. Kim, S. Fushinobu, S. Zhou, T. Wakagi, and H. Shoun
    • Journal Title

      Appl. Environ. Microbiol

      Volume: 75 Pages: 2652-2658

    • DOI

      doi:10.1128/AEM.02536-08

  • [Journal Article] Cloning and characterization of two flavohemoglobins from Aspergillus oryzae2009

    • Author(s)
      S. Zhou, S. Fushinobu, Y. Nakanishi, S.-W. Kim, T. Wakagi, and H. Shoun
    • Journal Title

      Biochem. Biophys. Res. Comm

      Volume: 381 Pages: 7-11

    • DOI

      doi:10.1016/j.bbrc.2009.01.112

  • [Presentation] 放線菌の新規窒素代謝経路の解明2012

    • Author(s)
      小口悠、佐々木康幸、小林卓矢、高谷直樹、矢嶋俊介、池田治生、高野英晃、大澤貫寿、祥雲弘文
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-24
  • [Presentation] 嫌気条件下におけるStreptomyces coelicolorのプロテオーム解析2010

    • Author(s)
      石山正宗、矢嶋俊介、大澤貫寿、祥雲弘文、佐々木康幸
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
  • [Presentation] 放線菌における異化型硝酸塩還元酵素の関連した新規窒素代謝系の解析2010

    • Author(s)
      山崎良、佐々木康幸、高谷直樹、矢嶋俊介、池田治生、高野英晃、大澤貫寿、祥雲弘文
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
  • [Presentation] 放線菌における新規窒素代謝系の解析2010

    • Author(s)
      佐々木康幸、森谷健太、高谷直樹、山崎良、矢嶋俊介、大澤貫寿、祥雲弘文
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Year and Date
      2010-03-29
  • [Presentation] 放線菌由来フラボヘモグロビンパラログに関する研究2010

    • Author(s)
      加藤慶一、池田宗一郎、吉本明弘、黒木進吾、矢嶋俊介、池田治生、大澤貫寿、祥雲弘文、佐々木康幸
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Year and Date
      2010-03-28

URL: 

Published: 2014-08-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi