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2012 Fiscal Year Final Research Report

Molecular mechanism of bacterial signal transduction

Research Project

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Project/Area Number 20248012
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionKinki University

Principal Investigator

UTSUMI Ryutaro  近畿大学, 農学部, 教授 (20151912)

Project Period (FY) 2008 – 2012
Keywordstwo-component system / SafA / EvgS/EvgA / PhoQ/PhoP / connector
Research Abstract

Bacteria have evolved two-component signal transduction systems (TCS), which are comprisedof sensor histidine kinases(HK) and theircognate response regulators(RR, transcriptional factor). Bacterial cells have a number of TCSs to respond to various environmental stresses. Comprehensive transcriptome studies of these TCSs suggest that they interact with each other by means of a signal transduction network. In Escherichia colicells, we haveclarified the molecular mechanism of how EvgS/EvgA TCS connects to PhoQ/PhoP TCS via a connector SafA.

  • Research Products

    (40 results)

All 2013 2012 2011 2010 2009 2008 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (8 results) Book (6 results) Remarks (6 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 1 results)

  • [Journal Article] The activation mechanism of PhoQ/PhoP system by SafA2013

    • Author(s)
      Ishii E, Eguchi Y, and Utsumi R
    • Journal Title

      an auxiliary protein of histidine kinase in Escherichia coli. Biosci Biotechnol Biochem

      Volume: (in press)

    • URL

      https://www.jstage.jst.go.jp/article/bbb/73/4/73_80795/_article

    • Peer Reviewed
  • [Journal Article] Waldiomycin, a novel WalK-histidine kinase inhibitor from Streptomyces sp.2013

    • Author(s)
      Igarashi M, Watanabe T, Hashida T, Umekita M, Hatano M, Yanagita Y, Kino H, Kimura T, Kinoshita N, Inouye K, Sawa R, Nishimura Y,Utsumi R, and Nomoto A
    • Journal Title

      MK844-mF10.

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Aconnecter-like factor, CacA, linksRssB/RpoS and the CpxR/CpxA two-component system in Salmonella2012

    • Author(s)
      Kato A, Hayashi H, Nomura W, Emori H,Hagihara K, and Utsumi R
    • Journal Title

      BMC Microbiol

      Volume: 12 Pages: 224-235

    • DOI

      doi:10.1186/1471-2180-12-224.

    • Peer Reviewed
  • [Journal Article] Isolation and characterization of signermycin B, an antibiotic that targets the dimerization domain of histidine kinase WalK.2012

    • Author(s)
      Watanabe T, Igarashi M, Okajima T, Ishii E, Kino H, Hatano, M, Sawa R, Umekita M, Kimura T, Okamoto S, Eguchi Y, Akamatsu Y, and Utsumi R
    • Journal Title

      An timicrob Agen ts Ch emoth er

      Volume: 56 Pages: 3657-3663

    • DOI

      doi:10.1128/AAC.06467-11.

    • Peer Reviewed
  • [Journal Article] The connector SafA interacts with the multi-sensing domain of PhoQ2012

    • Author(s)
      Eguchi, Y, lshii, E.,Yamane, M., and Utsumi, R
    • Journal Title

      Escherichia soli. Mol Microbiol

      Volume: 85 Pages: 299-313

    • DOI

      doi:10.1111/j.1365-2958.2012.08114.x.

    • Peer Reviewed
  • [Journal Article] A short-chain dehydrogenase involved in terpene metabolism from Zinglber zerembet.2011

    • Author(s)
      Okamoto S, Yu F, Harada H, Okajima T, Misawa N, and Utsumi R.
    • Journal Title

      FEBS J

      Volume: 278 Pages: 2892-2900

    • DOI

      doi:10.1111/j.1742-4658.2011.08211.x.

    • Peer Reviewed
  • [Journal Article] Development of an Antivirulence Drug against Streptococcus mutans2011

    • Author(s)
      Eguchi Y, Kubo N, Matsunaga H, Igarashi M, and Utsumi R
    • Journal Title

      Repression of Biofilm Formation, Acid Tolerance, and Competence by a Histidine Kinase Inhibitor, Walkmycin C.Antimicrob Agents Chemother 55

      Pages: 1475-84

    • DOI

      doi:10.1128/AAC.01646-10

    • Peer Reviewed
  • [Journal Article] Regulation of acid resistance by connectors of two component signal transduction systems2011

    • Author(s)
      Eguchi Y, lshii E, Hata K, and Utsumi R
    • Journal Title

      Escherichia coli. J Bacteriol

      Volume: 193 Pages: 1222-8

    • DOI

      doi:10.1128/JB.01124-10.

    • Peer Reviewed
  • [Journal Article] X-ray crystal structure of the DNA-binding domain of response regulator WalR essential to the cell viability of Staphylococcus aureus and interaction with target DNA2010

    • Author(s)
      Doi A, Okajima T, Gotoh Y, Tanizawa K, and Utsumi R
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 74 Pages: 1901-7

    • URL

      https:〃www.j stage.j st.go.jp/article/bbb/74/9/74 100307/ article

    • Peer Reviewed
  • [Journal Article] Walkmycin B targets WalK (YycG), a histidine kinase essential for bacterial cell growth.2010

    • Author(s)
      Okada A, lgarashi M, Okajima T, Kinoshita N, Umekita M, Sawa R, Inoue K, Watanabe T, Doi A, Martin A, Quinn J, Nishimura Y, and Utsumi R
    • Journal Title

      JAntibiot (Tokyo)

      Volume: 63 Pages: 89-94

    • DOI

      doi:10.1038/ja.2009.128.

    • Peer Reviewed
  • [Journal Article] Novel antibacterial compounds specifically targeting the essential WalR response regulator2010

    • Author(s)
      Gotoh Y, Doi A, Furuta E, Dubrac S, Ishizaki Y, Okada M, Igarashi M, Misawa N, Yoshikawa H, Okajima T, Msadek T, and Utsumi R
    • Journal Title

      J An tibiot (Tokyo)

      Volume: 63 Pages: 127-134

    • DOI

      doi:10.1038/ja.2010.4.

    • Peer Reviewed
  • [Journal Article] Molecular mechanism of trans- criptional cascade initiated by the EvgS/EvgA system in Escherlchla coli K-122009

    • Author(s)
      Itou J, Eguchi Y, and Utsumi R
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 73 Pages: 870-878

    • URL

      https:〃www.jstage.jst.go.jp/article/bbb/73/4/73 80795/ article

    • Peer Reviewed
  • [Journal Article] Molecular cloning and functional characterization of a-humulene synthase, a possible key enzyme of zerumbone biosynthesis in shampoo ginger(Zingiber zerumbet Smith)2008

    • Author(s)
      Yu F, Okamoto S, Nakasone K, Adachi K,Matsuda S, Harada H, Misawa N, and Utsumi R
    • Journal Title

      Planto

      Pages: 1291-1299

    • DOI

      doi:10.1007/sOO425-008-0700-x.

    • Peer Reviewed
  • [Journal Article] Isolation and functional characterization of a S‐ eudesmol synthase, a new sesquiter pene synthase from Zingiber zerumbet Smith2008

    • Author(s)
      Yu F, Harada H, Yamasaki K, Okamoto S,Hirase S, Tanaka Y, Misawa N, and Utsumi R
    • Journal Title

      FEBS Lett

      Volume: 582 Pages: 565-572

    • DOI

      doi:10.1016/j.febslet.2008.01.020.

    • Peer Reviewed
  • [Journal Article] Response regulator YycF essential for bacterial growth X-ray crystal structure of the DNA-binding domain and its PhoB-like DNA recognition motif.2008

    • Author(s)
      Okajima T, Doi A, Okada A, Gotoh Y, Tanizawa K, and Utsumi R
    • Journal Title

      FEBS Lett

      Volume: 582 Pages: 3434-3438

    • DOI

      doi:10.1016/j.febslet.2008.09.007.

    • Peer Reviewed
  • [Presentation] 細菌情報伝達阻害型薬剤2013

    • Author(s)
      内海龍太郎
    • Organizer
      日本農芸化学会シンポジューム
    • Place of Presentation
      東北大学(宮城県)
    • Year and Date
      2013-03-27
  • [Presentation] The connector SafA interacts with the multi-sensing domain of PhoQ2012

    • Author(s)
      Eguchi Y
    • Organizer
      Gordon conference on Microbial Stress Response
    • Place of Presentation
      Mount Holyoke College(USA)
    • Year and Date
      20120615-20
  • [Presentation] 細菌情報伝達機構と新規抗菌剤の開発2012

    • Author(s)
      内海龍太郎
    • Organizer
      日本細菌学会シンポジューム
    • Place of Presentation
      長崎新聞文化ホール(長崎県)
    • Year and Date
      20120327-30
  • [Presentation] Antibacterial and antivirulent drugs targeting bacterial signal transduction2011

    • Author(s)
      Utsumi R
    • Organizer
      The commemorativeInternational conference for the 20thanniversary of Korean Society of Life Science
    • Place of Presentation
      Busan (Korea)
    • Year and Date
      20111027-28
  • [Presentation] EvgS/EvgA signal transduction: transcriptional cascade and acid resistance2010

    • Author(s)
      Utsumi R
    • Organizer
      Society for general microbiology Autumn Meeting
    • Place of Presentation
      Nottingham (UK)
    • Year and Date
      20100906-09
  • [Presentation] 細菌情報伝達ネットワークとその阻害剤の開発2009

    • Author(s)
      内海龍太郎
    • Organizer
      日本乳酸菌学会秋季セミナー
    • Place of Presentation
      東京農業大学(東京都)
    • Year and Date
      2009-11-27
  • [Presentation] 増殖必須のTCSを標的にした細菌情報伝達阻害剤の開発2008

    • Author(s)
      内海龍太郎
    • Organizer
      日本分子生物学会・生化学会合同大会シンポジューム"細菌情報伝達ネットワークとドラッグターゲット"
    • Place of Presentation
      神戸ポートピアアイランド(兵庫県)
    • Year and Date
      20081209-12
  • [Presentation] 細菌膜小蛋白質によるTCSネットワーク制御機構2008

    • Author(s)
      江口陽子
    • Organizer
      日本分子生物学会・生化学会合同大会シンポジューム"細菌情報伝達ネットワークとドラッグターゲット"
    • Place of Presentation
      神戸ポートピアアイランド(兵庫県)
    • Year and Date
      20081209-12
  • [Book] "コネクターがつなぐ細菌情報伝達ネットワーク"化学と生物2013

    • Author(s)
      江口陽子、加藤明宣、石井英治、内海龍太郎
    • Total Pages
      241-249
    • Publisher
      日本農芸化学会
  • [Book] "Molecular mechanism of bacterial two-component signal transduction networks via connectors"in Two-component System in Bacteria2012

    • Author(s)
      Eguchi Y, Ishi E, Utsumi R
    • Total Pages
      149-162
    • Publisher
      Horizon Scientific Press
  • [Book] 病原細菌に対する新しい薬剤標的-二成分情報伝達システム2012

    • Author(s)
      内海龍太郎 五十嵐雅之
    • Total Pages
      51-58
    • Publisher
      YAKUGAKU ZASSHI
  • [Book] 細菌情報伝達ネットワークとその阻害剤の開発2010

    • Author(s)
      江口陽子 内海龍太郎
    • Total Pages
      36-41
    • Publisher
      Jap.J.Lactic.acid.Bacteria(日本乳酸菌学会)
  • [Book] Two-component signal transduction as potential drug targets in pathogenic bacteria2010

    • Author(s)
      Gotoh Y, Eguchi Y, Watanabe T, Okamoto S, Doi A, and Utsumi R
    • Total Pages
      232-239
    • Publisher
      Curr Opin Microbiol
    • URL

      doi: 10.1016/j.mib.2010.01.008.

  • [Book] "Bacterial Signal transduction: networks and drug targets"2008

    • Author(s)
      UtsumiR
    • Total Pages
      242
    • Publisher
      Springer
  • [Remarks] Bio-protocol(実験手法データベース)

  • [Remarks] Protein-peptide Interaction by Surface Plasmon Resonance (2013)

  • [Remarks] Eiji Ishii, Yoko Eguchi and Ryutaro Utsumi

  • [Remarks]

    • URL

      http://www.bio-protocol.org/wenzhang.aspx?id=321

  • [Remarks] 近畿大学研究業績データーベース

    • URL

      http://rais.itp.kindai.ac.jp/researchdb

  • [Remarks] 近畿大学農学部分子生物学研究室

    • URL

      http://mbkinki.sakura.ne.jp/index.html

  • [Patent(Industrial Property Rights)] セスキテルペンシンターゼ活性を有するポリペプチドをコードする核酸2013

    • Inventor(s)
      内海龍太郎
    • Industrial Property Rights Holder
      近畿大学
    • Industrial Property Number
      特許,5219025
    • Acquisition Date
      2013-03-15
  • [Patent(Industrial Property Rights)] 化合物、その互変異性体、幾何異性体、ないし、それらの塩、及びそれらの製造方法、抗菌剤、並びにその感染症治療薬2012

    • Inventor(s)
      内海龍太郎他3名
    • Industrial Property Rights Holder
      近畿大学他3
    • Industrial Property Number
      特許,2012-230270
    • Filing Date
      2012-10-17
  • [Patent(Industrial Property Rights)] Compound signermycin, method for production thereof, and use thereof2011

    • Inventor(s)
      内海龍太郎 五十嵐雅之
    • Industrial Property Rights Holder
      近畿大学
    • Industrial Property Number
      特許,US8058455B2
    • Acquisition Date
      2011-11-15
    • Overseas
  • [Patent(Industrial Property Rights)] 新規化合物、ウオークマイシン、その製造方法、及びその用途2009

    • Inventor(s)
      内海龍太郎五 十嵐雅之
    • Industrial Property Rights Holder
      近畿大学. 微生物化学研究会
    • Industrial Property Number
      特許,2009-186724
    • Filing Date
      2009-08-11
  • [Patent(Industrial Property Rights)] 新規化合物、シグナマイシン、その製造方法、及びその用途2008

    • Inventor(s)
      内海龍太郎 五十嵐雅之
    • Industrial Property Rights Holder
      近畿大学微生物化学研究会
    • Industrial Property Number
      特許,PCT/Jp2008/065196
    • Filing Date
      2008-08-26

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Published: 2014-08-29  

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