Search for Novel Functionalities of Amino Acids as Bio-regulatory Factors and Verification of Their Usefulness

2010 Fiscal Year Final Research Report

• Project/Area Number: 20248014
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Niigata University
• Principal Investigator: KADOWAKI Motoni 新潟大学, 自然科学系, 教授 (90126029)
• Co-Investigator(Kenkyū-buntansha): KATO Hisanori 東京大学, 総括プロジェクト機構, 特任教授 (40211164) ; YOSHIZAWA Fumiaki 宇都宮大学, 農学部, 教授 (10269243) ; ODA Hiroaki 名古屋大学, 生命農学研究科, 准教授 (20204208) ; FURUYA Shigeki 九州大学, 農学研究院, 教授 (00222274) ; FUJIMURA Shinobu 新潟大学, 自然科学系, 准教授 (20282999)
• Project Period (FY): 2008 – 2010
• Keywords: アミノ酸 / マイクロアレイ / 発現制御 / 栄養学 / シグナル伝達

Research Abstract

Collaborative works on the signal transduction and cell response to amino acids were undertaken mainly by using DNA microarray analysis. Thus, functionalities of amino acids, not as nutrients, but as signaling molecules, were analyzed regarding the regulation of protein synthesis and degradation, i. e., gene expression and autophagy, the regulations of carbohydrate metabolism and lipid metabolism, and a signaling mechanism of serine availability in the neuronal tissues. Complex information network structures of a variety of amino acids were partially clarified.

Research Products

• [Journal Article] Mild caloric restriction up-regulates the expression of prohibitin : A proteome study (2011)
  • Author(s): Takahashi, S., Masuda, J., Shimagami, H., Ohta, Y., Kanda, T., Saito, K. and Kato, H
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 405 Pages: 462-467
  • Peer Reviewed
• [Journal Article] The fat and protein fractions of freshwater clam(Corbicula fluminea) extract reduce serum cholesterol, and enhance bile acid biosynthesis and sterol excretion in hypercholesterolaemic rats fed a high-cholesterol diet (2011)
  • Author(s): Chijimatsu, T., Umeki, M., Okuda, Y., Yamada, K., Oda, H. and Mochizuki, S
  • Journal Title: Brit. J. Nutr Volume: 105 Pages: 526-534
  • Peer Reviewed
• [Journal Article] Regulation of muscular glutamate metabolism by high-protein diet in broiler chicks (2011)
  • Author(s): Kobayashi, H., Eguchi, A., Takano, W., Shibata, M., Kadowaki, M., and Fujimura, S
  • Journal Title: Anim. Sci. J. Volume: 82 Pages: 86-92
  • Peer Reviewed
• [Journal Article] Role of the renin-angiotensin-aldosterone system in the enhancement of salt sensitivity caused by prenatal protein restriction in stroke-prone spontaneously hypertensive rats (2011)
  • Author(s): Otani, L., Sugimoto, N., Kaji, M., Murai, M., Chang, S.-J., Kato, H. and Murakami, T
  • Journal Title: J. Nutr. Biochem Volume: (in press)
  • Peer Reviewed
• [Journal Article] An Optimized Method for the Determination of Free L-Cysteine in Rat Plasmaby High-Performance Liquid Chromatography Using the Derivatization Reagent 4-Aminosulfonyl-7-Fluoro-2, 1, 3-Benzoxadiazole (2011)
  • Author(s): Otani, L., Ogawa, S., Zhao, Z., Nakazawa, K., Umehara, S., Yoshimura, E., Chang, S.-J. andKato, H
  • Journal Title: Biosci. Biotechnol. Biochem Volume: (in press)
  • Peer Reviewed
• [Journal Article] Vitamin E as a novel enhancer of macroautophagy in rat hepatocytes and H4-II-E cells (2010)
  • Author(s): Karim, MR., Fujimura, S., and Kadowaki, M
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 394 Pages: 981-987
  • Peer Reviewed
• [Journal Article] Improvement in the In Vivo Digestibility of Rice Protein by Alkali Extraction is Due to Structural Changes in Prolamin/Protein Body-Iparticle (2010)
  • Author(s): Kubota, M., Saito, Y., Masumura, T., Kumagai, T., Watanabe, R., Fujimura, S., and Kadowaki, M
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 74 Pages: 614-619
  • Peer Reviewed
• [Journal Article] Dietary protein deprivation up-regulatesinsulin signaling and in hibits gluconeogenesis in rat liver (2010)
  • Author(s): Toyoshima, Y, Tokita, R., Ohne, Y., Hakuno, F., Noguchi, T., Minami, S., Kato, H. and Takahashi, S. I
  • Journal Title: J. Mol. Endocrinol Volume: 45 Pages: 329-340
  • Peer Reviewed
• [Journal Article] Dietaryiron deficiency induces a variety of metabolic changes and even apoptosis in rat liver : a DNA microarray study (2010)
  • Author(s): Kamei, A., Watanabe, Y., Ishijima, T., Uehara, M., Arai, S., Kato, H., Nakai, Y. and Abe, K
  • Journal Title: Physiol. Genomics Volume: 42 Pages: 149-156
  • Peer Reviewed
• [Journal Article] 分枝鎖アミノ酸の代謝調節因子としての機能 (2010)
  • Author(s): 吉澤*史昭
  • Journal Title: 栄養生理研究会報 Volume: 54 Pages: 9-20
  • Peer Reviewed
• [Journal Article] Advantages of human hepatocyte-derivedtrans formants expressing a series of humancytochrome P-450 isoforms for genotoxicity examination (2010)
  • Author(s): Hashizume, T., Yoshitomi, S., Asahi, S., Uematsu, R., Matsumura, S., Chatani, F. and Oda, H
  • Journal Title: Toxicol. Sci Volume: 116 Pages: 488-497
  • Peer Reviewed
• [Journal Article] Brainspecific Phgdh deletion reveals a pivotal role for L-serine biosynthesis in controlling the level of D-serine (2010)
  • Author(s): Yang, JH., Wada, A., Yoshida, K., Miyoshi, Y., Sayano, T., Esaki, K., Kinoshita, O. M., Tomonaga, S., Azuma, N., Watanabe, M., Hamase, K., Zaitsu, K., Machida, T., Messing, A., Itohara, S., Hirabayashi, Y., Furuya, S
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 41380-41390
  • Peer Reviewed
• [Journal Article] 遺伝性セリン合成不全疾患モデルマウスの脳内遊離アミノ酸組成に及ぼす大豆ペプチド摂取効果の定量的検討 (2010)
  • Author(s): 古屋茂樹
  • Journal Title: 大豆タンパク質研究 Volume: 13 Pages: 109-115
• [Journal Article] アミノ酸研究の最前線I (2010)
  • Author(s): 古屋茂樹
  • Journal Title: 外科と代謝・栄養 Volume: 44 Pages: 65-71
• [Journal Article] Effects of Rice Proteins from Two Cultivars, Koshihikari and Shunyo (2009)
  • Author(s): Yang, L., and Kadowaki, M. HepaticCholesterol Secretion by Isolated PerfusedLivers of Rats fed Cholesterol-Enriched Diets
  • Journal Title: Ann. Nutr. Metab. Volume: 54 Pages: 283-290
  • Peer Reviewed
• [Journal Article] Superiority of Alkali-Extracted Rice Proteinin Bioavailablity to Starch Degraded Rice Protein Comes from Digestion of Prolamin in Growing Rats (2009)
  • Author(s): Kumagai, T., Watanabe, R., Saito, M., Watanabe, T., Kubota, M., and Kadowaki, M
  • Journal Title: J. Nutr. Sci. Vitaminol. Volume: 55 Pages: 170-177
  • Peer Reviewed
• [Journal Article] Cytosolic LC3 ratio as a quantitative index of macroautophagy (2009)
  • Author(s): Kadowaki, M., Karim, MR
  • Journal Title: Methods in Enzymology Volume: Vol.452 Pages: 199-213
  • Peer Reviewed
• [Journal Article] A freshwater clam(Corbicula fluminea) extract reduces cholesterol level and hepatic lipids in normal rats and xenobioticsinduced hypercholesterolemic rats (2009)
  • Author(s): Chijimatsu, T., Tatsuguchi, I., Oda, H. andMochizuki, S
  • Journal Title: J. Agric. Food Chem. Volume: 57 Pages: 3108-3112
  • Peer Reviewed
• [Journal Article] In vitro micronucleus test in HepG2 transformants expressing a series of human cytochrome P450 isoforms with chemicals requiring metabolic activation (2009)
  • Author(s): Hashizume, T., Yoshitomi, S., Asahi, S., Matsumura, S., Chatani, F. and Oda, H
  • Journal Title: Mutation Res. Volume: 677 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Regular feeding plays an important role in cholesterol homeostasis through the liver circadian clock (2009)
  • Author(s): Yamajuku, D., Okubo, S., Haruma, T., Inagaki, T., Okuda, Y., Kojima T, Noutomi, K., Hashimoto, S. and Oda, H
  • Journal Title: Circulation Res. Volume: 105 Pages: 545-548
  • Peer Reviewed
• [Journal Article] Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes (2008)
  • Author(s): Klionsky, DL, Kadowaki, M.
  • Journal Title: Autophagy Volume: 4 Pages: 151-175
  • Peer Reviewed
• [Journal Article] Regulation of taste-active components of meatby dietary branched-chain amino acids : effects ofbranched-chain amino acid antagonism (2008)
  • Author(s): Kadowaki, M., Fujimura, S
  • Journal Title: Br. Poultry Sci. Volume: 49(3) Pages: 299-307
  • Peer Reviewed
• [Book] 網羅的遺伝子発現解析を利用した食品の安全性評価、「食の安全科学の展開-食のリスク予測と制御に向けて」(東京大学食の安全研究センター編) (2010)
  • Author(s): 加藤久典
  • Total Pages: 98-102
  • Publisher: シーエムシー出版
• [Book] 食品データベース(第4章食品分野への応用)、「バイオチップ実用化ハンドブック」(金子周一、堀池靖浩編) (2010)
  • Author(s): 加藤久典、斉藤憲司
  • Total Pages: 346-352
  • Publisher: エヌ・ティー・エス
• [Book] 窒素化合物の代謝「現代栄養学を理解するための分子生物学入門」 (2010)
  • Author(s): 加藤久典
  • Total Pages: 106-114
  • Publisher: 光生館
• [Remarks] ニュートリゲノミクスデータベース
  • URL: http://nutrigenomics.jp/
• [Remarks] KEGGLEパスウェイ解析ツール
  • URL: http://keggle.jp/
• [Remarks] 第65回日本栄養・食糧学会大会イブニング・セミナー「栄養機能ゲノミクス研究会:DNAマイクロアレイをどのように使いこなすか?」を開催(2010年5月、徳島)