Genetic investigation of the genes responsible for hereditary disorders of Japanese Black cattle and its functional analysis

2010 Fiscal Year Final Research Report

• Project/Area Number: 20248029
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Okayama University
• Principal Investigator: KUNIEDA Tetsuo 岡山大学, 大学院・自然科学研究科, 教授 (80178011)
• Co-Investigator(Kenkyū-buntansha): TSUJI Takehito 岡山大学, 大学院・自然科学研究科, 准教授 (90314682)
• Co-Investigator(Renkei-kenkyūsha): UCHIDA Kazuyuki 東京大学, 大学院・農学生命科学研究科, 准教授 (10223554) ; MORITOMO Yasuo 東海大学, 農学部, 教授 (70148972) ; YAMATO Osamu 鹿児島大学, 農学部, 教授 (80261337) ; WATANABE Daisaku 北里大学, 獣医学部, 准教授
• Project Period (FY): 2008 – 2010
• Keywords: 育種 / 和牛 / 遺伝性疾患 / 遺伝子診断

Research Abstract

We have attempted to identify the gene responsible for hereditary disorders of Japanese Black cattle and develop the genetic diagnosis systems to detect the mutations. Consequently, we have revealed that a nonsense mutation of the GFRA1 gene is responsible for forelimb-girdle muscular anomaly and developed the genetic diagnosis systems for this disorder by PCR-RFLP method. For other disorders including short mandible and renal agenesis, we have collected samples of affected animals and performed linkage analysis using these samples. As a result, the locus for short mandible and renal agenesis was mapped on a particular region of bovine chromosome. These finding can be used to develop genetic diagnosis system for this disorder.

Research Products

• [Journal Article] 見島牛における遺伝性疾患、毛色、経済形質に関わる遺伝子座の対立遺伝子の分布 (2010)
  • Author(s): 米田一裕、渡辺望、国枝哲夫
  • Journal Title: 動物遺伝育種学研究 Volume: 38 Pages: 13-19
• [Journal Article] A mutation of the WFDC1 gene is responsible for multiple ocular defects in cattle Abbasi AR (2009)
  • Author(s): Khalaj M, Tsuji T, Tanahara M, Uchida K, Sugimoto Y, Kunieda T
  • Journal Title: Genomics Volume: 94 Pages: 55-62
• [Journal Article] A missense mutation(p. Leu2153His) of the factor VIII gene causes cattle haemophilia A (2009)
  • Author(s): Khalaj M, Abbasi AR, Shimojo K, Moritomo Y, Yoneda K, Kunieda T
  • Journal Title: Anim Genet Volume: 40 Pages: 763-765
• [Journal Article] Exclusion of NEU1 and PPGB from candidate genes for a lysosomal storagedisease in Japanese Black cattle (2009)
  • Author(s): Masoudi, A. A., Yamato, O., Yoneda, K., Tsuji, T., Mikami, O., Kunieda, T.
  • Journal Title: Anim Sci J Volume: 80 Pages: 611-615
• [Journal Article] Linkage mapping of the locus responsible for forelimb-girdle muscular anomaly of Japanese Black cattle on bovine chromosome 26 (2008)
  • Author(s): Masoudi, A. A., Uchida, K., Yokouchi, K., Ohwada, K., Abbasi, A. R., Tsuji, T., Watanabe, T., Hirano, T., Sugimoto, Y., and Kunieda, T.
  • Journal Title: Anim Genet Volume: 39 Pages: 46-50
• [Journal Article] Clinical and Pathological Aspects of Hemophilia A in Japanese Brown Cattle. (2008)
  • Author(s): Moritomo, Y., Shimojo, K., Miyadera, K., Khalaj, M., Asano, Kunieda, T., Ogawa, H.
  • Journal Title: J Vet Med Sci. Volume: 70 Pages: 293-296
• [Journal Article] Pedigree Analysis of Factor XI Deficiency in Japanese Black Cattle. (2008)
  • Author(s): Ohba, Y., Yakasu, M., Nishii, N., Takeda, E., Maeda, S., Kunieda, T., Kitagawa, H.
  • Journal Title: J Vet Med Sci. Volume: 70 Pages: 297-299
• [Presentation] 見島牛における和牛の諸形質に関連する遺伝子変異の解析 (2009)
  • Author(s): 渡辺望、辻岳人、国枝哲夫
  • Organizer: 日本動物遺伝育種学会第10回大会
  • Place of Presentation: 前橋
  • Year and Date: 20091109-10
• [Presentation] 黒毛和種における血液凝固第XI因子欠乏症 (2009)
  • Author(s): 小林直彦、松橋珠子、坂口慎一、加藤勉、国枝哲夫
  • Organizer: 日本動物遺伝育種学会第10回大会
  • Place of Presentation: 前橋
  • Year and Date: 20091109-10
• [Presentation] 褐毛和種にみられた血液凝固不全症(血友病A)におけるF8遺伝子の変異 (2009)
  • Author(s): Khalaj Maryam、下城研一、宮寺恵子、Abdol Rahim Abbasi、米田一裕、小川博之、森友靖生、国枝哲夫
  • Organizer: 日本動物遺伝育種学会第10回大会
  • Place of Presentation: 前橋
  • Year and Date: 20091109-10
• [Presentation] ウシ疾病関連遺伝子、抗病性関連遺伝子の多様性解析 (2009)
  • Author(s): 金田真、笹崎晋史、国枝哲夫、万年英之
  • Organizer: 日本畜産学会第111回大会
  • Place of Presentation: 沖縄
  • Year and Date: 20090928-29