Biological Regulation of GlcNAc cycle

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20249018
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: The Institute of Physical and Chemical Research (2010-2013); Osaka University (2008-2009)
• Principal Investigator: TANIGUCHI Naoyuki Osaka University, 産業科学研究所・寄附研究部門, 教授 (90002188)
• Project Period (FY): 2008-04-08 – 2013-03-31
• Keywords: 糖鎖 / 糖ヌクレオチド / 糖鎖サイクル / グルコサミン転移酵素 / 細胞膜受容体 / オルガネラ / シグナル

Research Abstract

糖タンパク質のN結合型糖鎖がもつ糖鎖機能を糖鎖サイクルとして統合的に解析することにより、その生理的、病態的意義を明らかにする。我々はこれまで糖転移酵素の機能に着目してきたが、本研究はGlcNAcに関わる糖鎖サイクルに焦点を絞り、サイクルを形成する分子、1)単糖代謝、2)糖ヌクレオチド、3)糖ヌクレオチド輸送体、4)糖転移酵素、5)細胞膜受容体糖鎖、それぞれの機能を解明し、最終的にそれらを統合的に理解する。

Research Products

• [Journal Article] Hypoxic regulation of glycosylation via the N-acetylglucosamine cycle.(総説) (2011)
  • Author(s): Shirato K, Nakajima K, Korekane H, Takamatsu S, Gao C, Angata T, Ohtsubo K, Taniguchi N.
  • Journal Title: J.Clin.Biochem.Nutr. 48(1) Pages: 20-25
  • Peer Reviewed
• [Journal Article] Glycomic analyses of glycoproteins in bile and serum during rat hepatocarcinogenesis. (2010)
  • Author(s): Nakagawa T, Takeishi S, Kameyama A, Yagi H, Yoshioka T, Moriwaki K, Masuda T, Matsumoto H, Kato K, Narimatsu H, Taniguchi N, Miyoshi E.
  • Journal Title: J.Proteome Res. 9(10) Pages: 4888-4896
  • Peer Reviewed
• [Journal Article] Involvement of ST6Gal I in the biosynthesis of a unique human colon cancer biomarker candidate alpha2, 6-sialylated blood group type 2H (ST2H) antigen. (2010)
  • Author(s): Korekane H, Matsumoto A, Ota F, Hasegawa T, Misonou Y, Shida K, Miyamoto Y, Taniguchi N.
  • Journal Title: J.Biochem. 148(3) Pages: 359-370
  • Peer Reviewed
• [Journal Article] Physiological and Glycomic Characterization of N-acetylglucosaminyltransferase -IVa and -IVb Double Deficient Mice. (2010)
  • Author(s): Takamatsu S, Antonopoulos A, Ohtsubo K, Ditto D, Chiba Y, Le DT, Morris HR, Haslam SM, Dell A, Marth JD, Taniguchi N.
  • Journal Title: Glycobiology 20(4) Pages: 485-97
  • Peer Reviewed
• [Journal Article] Simultaneous Adetermination of nucleotide sugars with ion-pair reversed-phase HPLC. (2010)
  • Author(s): Nakajima K, Kitazume S, Angata T, Fujinawa R, Ohtsubo K, Miyoshi E, Taniguchi N.
  • Journal Title: Glycobiology 20(7) Pages: 865-871
  • Peer Reviewed
• [Presentation] Role of "glycan cycles" for understanding the role of glycan in disease (2011)
  • Author(s): Taniguchi N
  • Organizer: Ixtlt International Symposium on "Biochemical Roles of Eukaryotic Cell Surface Macromolecules"
  • Place of Presentation: エスチュアリーアイランドリゾート(トリバンドラム・インド)(招待講演)
  • Year and Date: 2011-01-30
• [Presentation] Role of N-glycans in disease (2011)
  • Author(s): Taniguchi N
  • Organizer: International Symposium : Glyco-biomarker for Cancer : as targets for early diagnosis and therapeutics
  • Place of Presentation: KRIBB(大田市・大韓民国)(招待講演)
  • Year and Date: 2011-01-28
• [Presentation] Novel Analytical Methods for nucleotide sugar metabolites (2010)
  • Author(s): Nakajima K, Ohtsubo K, Takamiya R, Shirato K, Kitazume S, Angata T, Taniguchi N
  • Organizer: 2010 RIKEN Chemical Biology International Symposium
  • Place of Presentation: 理化学研究所(埼玉県)(招待講演)
  • Year and Date: 2010-10-26
• [Presentation] Role of glycans in cancer(がんと糖鎖) (2010)
  • Author(s): 谷口直之
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場/リーガロイヤルホテル大阪(大阪府)(招待講演)
  • Year and Date: 2010-09-24
• [Presentation] Significance of Nucleotide Sugar Metabolism for Understanding Functional Glycomics by Using Ion-Pair Reversed-Phase HPLC and LC-ESI-MS (2010)
  • Author(s): Taniguchi N
  • Organizer: HUPO 2010 World Congress
  • Place of Presentation: コンベンションアンドエキシビジョンセンター(シドニー・オーストラリア)
  • Year and Date: 2010-09-20
• [Book] Capillary Electrophoresis of Carbohydrates (Capillary Electrophoresis and Capillary Electrophoresis-Mass Spectrometry for Structural Analysis of N-Glycans Derived from Glycoproteins) (2011)
  • Author(s): Nakano M, Kakehi K, Taniguchi N, Kondo A
  • Total Pages: 205-235
  • Publisher: Human Press