2013 Fiscal Year Final Research Report
Biological Regulation of GlcNAc cycle
| Project/Area Number |
20249018
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | The Institute of Physical and Chemical Research (2010-2013)
Osaka University (2008-2009) |
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Principal Investigator |
TANIGUCHI Naoyuki 独立行政法人理化学研究所, システム糖鎖生物学研究グループ, グループディレクター (90002188)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAJIMA Kazuki (独)理化学研究所, 脳科学総合研究センター神経膜機能研究チーム, 研究員 (10442998)
OHTSUBO Kazuaki 熊本大学, 大学院生命科学研究部 先端生命医療科学部門 医療技術科学講座 (30525457)
KOREKANE Hiroaki (独)理化学研究所, グローバル研究クラスタ 理研-マックスプランク連携研究センター システム糖鎖生物学研究グループ疾患糖鎖研究チーム, 研究員 (50421912)
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| Project Period (FY) |
2008-04-08 – 2013-03-31
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| Keywords | 糖鎖修飾 / 糖ヌクレオチド / 糖転移酵素 |
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| Research Abstract |
We proposed a fundamental paradigm"GlcNAc cycle" that living cell regulates biosynthesis and catabolism of glycans in response to pathological changes. We could grasp the dynamic cellular information by analyzing the each step in glycosylation. Nucleotide sugars level were simultaneously determined by ion-pair HPLC. Mass isotopomer analysis of metabolically labeled nucleotide sugars and N- and O-glycans revealed the UDP-GlcNAc metabolisms. We also developed a HPLC method for various GlcNAc transferases activities, and found the endogenous inhibitory factor for GlcNAc transferase-IX (GnT-IX) in Neuro2a cells. Brain-specific expression of the GnT-IX was also regulated by epigenetic histone modifications. In addition, GnT-III compensated for the loss of core fucose functions. These data suggested that glycosylation is regulated by multiple stages in cellular GlcNAc metabolisms.
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[Journal Article] Identification of Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 (ENPP3) as a Regulator of N-Acetylglucosaminyltransferase GnT-IX (GnT-Vb)2013
Author(s)
Korekane H, Park JY, Matsumoto A, Nakajima K, Takamatsu S, Ohtsubo K, Miyamoto Y, Hanashima S, Kanekiyo K, Kitazume S, Yamaguchi Y, Matsuo I, Taniguchi N
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Journal Title
J. Biol. Chem.
Volume: 288(39)
Pages: 27912-27926
DOI
Peer Reviewed
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[Journal Article] A Single Dose of LPS into Mice with Emphysema Mimics Human COPD Exacerbation as Assessed by Micro-CT2013
Author(s)
Kobayashi S, Fujinawa R, Ota F, Kobayashi S, Angata T, Ueno M, Maeno T, Kitazume S, Yoshida K, Ishii T, Gao C, Ohtsubo K, Yamaguchi Y, Betsuyaku T, Kida K, Taniguchi N
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Journal Title
Am. J. Respir. Cell Mol. Biol.
Volume: 49(6)
Pages: 971-7
DOI
Peer Reviewed
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[Journal Article] Loss of branched O-mannosyl glycans in astrocytes accelerates remyelination2013
Author(s)
Kanekiyo K, Inamori K, Kitazume S, Sato K, Maeda J, Higuchi M, Kizuka Y, Korekane H, Matsuo I, Honke K, Taniguchi N
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Journal Title
J. Neurosci.
Volume: 33(24)
Pages: 10037-10047
DOI
Peer Reviewed
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