A novel WNK signal cascade regulating renal transporters

2012 Fiscal Year Final Research Report

• Project/Area Number: 20249047
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: UCHIDA Shinichi 東京医科歯科大学, 大学院・医歯学総合研究科, 准教授 (50262184)
• Project Period (FY): 2008 – 2012
• Keywords: 水・電解質代謝学 / 塩分感受性高血圧 / 遺伝子改変マウス

Research Abstract

In 2007, we discovered a novel signal cascade of WNK kinases, and clarified that its constitutive activation causes human hypertension in pseudohypoaldosteronism type II (PHAII). This study was conducted to further clarify the roles of WNK signal cascade in vivo other than PHAII by using the genetically engineered mice. As a result, we were able to identify several hormonal factors that regulate WNK kinases and new downstream targets, and could provide a whole picture of WNK kinase signaling in the body, especially in kidney.

Research Products

• [Journal Article] ImpairedKLHL3-Mediated Ubiquitination of WNK4 Causes Human Hypertension (2013)
  • Author(s): Wakabayashi M, Mori T, Isobe K, Sohara E, Susa K, Araki Y, Chiga M, Kikuchi E, NomuraN, Mori Y, Matsuo H, Murata T, Nomura S, Asano T, Kawaguchi H, Nonoyama S, Rai T, Sasaki S, Uchida S
  • Journal Title: Cell Rep Volume: 3(3) Pages: 858-68
  • Peer Reviewed
• [Journal Article] Involvement of aquaporin-7 inthe cutaneous primary immune response through modulation of antigen uptake and migration in dendritic cells (2012)
  • Author(s): Hara-Chikuma M, Sugiyama Y, Kabashima K, Sohara E, Uchida S, Sasaki S, Inoue S, Miyachi Y
  • Journal Title: FASEB J Volume: 26 Pages: 211-8
  • Peer Reviewed
• [Journal Article] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination (2012)
  • Author(s): Hossain Khan MZ, Sohara E, Ohta A, ChigaM, Inoue Y, Isobe K, Wakabayashi M, Oi K, Rai T, Sasaki S, Uchida S
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 425 Pages: 456-61
  • Peer Reviewed
• [Journal Article] A new role for aquaporin 7 in insulin secretion (2012)
  • Author(s): Louchami K, Best L, Brown P, Virreira M, Hupkens E, Perret J, Devuyst O, Uchida S, Delporte C, Malaisse WJ, Beauwens R, SenerA
  • Journal Title: Cell Physiol. Biochem Volume: 29 Pages: 65-74
  • Peer Reviewed
• [Journal Article] ASK3 responds to osmotic stress and regulates blood pressure by suppressing WNK1-SPAK/OSR1 signaling in the kidney (2012)
  • Author(s): Naguro I, Umeda T, Kobayashi Y, MaruyamaJ, Hattori K, Shimizu Y, Kataoka K,Kim-Mitsuyama S, Uchida S, Vandewalle A, Noguchi T, Nishitoh H, Matsuzawa A, Takeda K, Ichijo H
  • Journal Title: Nat. Commun Volume: 3 Pages: 1285
  • Peer Reviewed
• [Journal Article] Phosphatidylinositol 3-kinase/Akt signaling pathway activates theWNK-OSR1/SPAK-NCC phosphorylation cascade in hyperinsulinemic db/db mice (2012)
  • Author(s): Nishida H, Sohara E, Nomura N, Chiga M, Alessi DR, Rai T, Sasaki S, Uchida S
  • Journal Title: Hypertension Volume: 60 Pages: 981-90
  • Peer Reviewed
• [Journal Article] Accumulation of autofluorescent storage material in brain is accelerated byischemia in chloride channel 3gene-deficient mice (2012)
  • Author(s): Ohtaki H, Ohara K, Song D, Miyamoto K, Tsumuraya T, Yofu S, Dohi K, Tanabe S, Sasaki S, Uchida S, Matsunaga M, Shioda S
  • Journal Title: J. Neurosci. Res Volume: 90 Pages: 2163-72
  • Peer Reviewed
• [Journal Article] A minor role of WNK3 in regulating phosphorylation ofrenal NKCC2 and NCC co-transporters in vivo (2012)
  • Author(s): Oi K, Sohara E, Rai T, Misawa M, Chiga M, Alessi DR, Sasaki S, Uchida S
  • Journal Title: Biol.Open Volume: 1 Pages: 120-7
  • Peer Reviewed
• [Journal Article] Effect of heterozygousdeletion of WNK1 on the WNK-OSR1/SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels (2012)
  • Author(s): Susa K, Kita S, Iwamoto T, Yang SS, LinSH, Ohta A, Sohara E, Rai T, Sasaki S, Alessi DR, Uchida S
  • Journal Title: Clin. Exp. Nephrol Volume: 16 Pages: 530-8
  • Peer Reviewed
• [Journal Article] WNK-OSR1/SPAK-NCC signal cascade has circadian rhythmdependent on aldosterone (2012)
  • Author(s): Susa K, Sohara E, Isobe K, Chiga M, Rai T, Sasaki S, Uchida S
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 427 Pages: 743-7
  • Peer Reviewed
• [Journal Article] Does a β2-adrenergicreceptor-WNK4-Na-Cl co-transporter signal cascade exist in the in vivo kidney? (2012)
  • Author(s): Uchida S, Chiga M, Sohara E, Rai T, Sasaki S
  • Journal Title: Nat.Med Volume: 18 Pages: 1324-5
  • Peer Reviewed
• [Journal Article] Phenotypes of pseudohypoaldosteronism type II caused by the WNK4 D561A missense mutation are dependent on theWNK-OSR1/SPAK kinase cascade (2011)
  • Author(s): Chiga M, Rafiqi FH, Alessi DR, Sohara E, Ohta A, Rai T, Sasaki S, Uchida S
  • Journal Title: J. Cell Sci Volume: 124 Pages: 1391-5
  • Peer Reviewed
• [Journal Article] Impaired phosphorylation of Na(+)-K(+)-2Cl(-) cotransporter by oxidative stress-responsive kinase-1deficiency manifests hypotension and Bartter-like syndrome (2011)
  • Author(s): Lin SH, Yu IS, Jiang ST, Lin SW, Chu P, Chen A, Sytwu HK, Sohara E, Uchida S, Sasaki S, Yang SS
  • Journal Title: Proc. Natl. Acad. Sci. U S A Volume: 108 Pages: 17538-43
  • Peer Reviewed
• [Journal Article] Regulation of WNK1 kinase by extracellular potassium (2011)
  • Author(s): Naito S, Ohta A, Sohara E, Ohta E, Rai T, Sasaki S, Uchida S
  • Journal Title: Clin. Exp. Nephrol Volume: 15 Pages: 195-202
  • Peer Reviewed
• [Journal Article] Generation and analyses of R8L barttin knockin mouse (2011)
  • Author(s): Nomura N, Tajima M, Sugawara N, Morimoto T, Kondo Y, Ohno M, Uchida K, Mutig K, Bachmann S, Soleimani M, Ohta E, Ohta A, Sohara E, Okado T, Rai T, Jentsch TJ, Sasaki S, UchidaS
  • Journal Title: Am. J. Physiol. Renal. Physiol Volume: 301 Pages: F297-307
  • Peer Reviewed
• [Journal Article] Dexamethasone increases the phosphorylation of nephrin in cultured podocytes (2011)
  • Author(s): Ohashi T, Uchida K, Uchida S, Sasaki S, Nitta K
  • Journal Title: Clin. Exp. Nephrol Volume: 15 Pages: 688-93
  • Peer Reviewed
• [Journal Article] Immunolocalization of WNK4 in mouse kidney (2011)
  • Author(s): Ohno M, Uchida K, Ohashi T, Nitta K, Ohta A, Chiga M, Sasaki S, UchidaS
  • Journal Title: Histochem. Cell Biol Volume: 136 Pages: 25-35
  • Peer Reviewed
• [Journal Article] Acute insulin stimulation induces phosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney (2011)
  • Author(s): Sohara E, Rai T, Yang SS, Ohta A, NaitoS, Chiga M, Nomura N, Lin SH, Vandewalle A, Ohta E, Sasaki S, Uchida S
  • Journal Title: PLoS One Volume: 6 Pages: e24277
  • Peer Reviewed
• [Journal Article] Phosphorylation of aquaporin-2 regulates its water permeability (2010)
  • Author(s): Eto K, Noda Y, Horikawa S, Uchida S, Sasaki S
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 40777-84
  • Peer Reviewed
• [Journal Article] Short-term stimulation of the thiazide-sensitive Na+-Cl- cotransporter by vasopressin involves phosphorylation and membrane translocation (2010)
  • Author(s): Mutig K, Saritas T, Uchida S, Kahl T,Borowski T, Paliege A, Bohlick A, Bleich M, Shan Q, Bachmann S
  • Journal Title: Am. J. Physiol. Renal Physiol Volume: 298 Pages: 502-9
  • Peer Reviewed
• [Journal Article] Calcium and calcimimetics regulate paracellular Na+ transport in the thin ascending limb of Henle's loop in mouse kidney (2010)
  • Author(s): Sugawara N, Morimoto T, Farajov EI, Kumagai N, Aslanova UF, Rai T, Uchida S, Sasaki S, Tsuchiya S, Kondo Y
  • Journal Title: Pflugers Arch Volume: 460 Pages: 197-205
  • Peer Reviewed
• [Journal Article] Effect of angiotensin II on the WNK-OSR1/SPAK-NCC phosphorylation cascade in cultured mpkDCT cells and in vivo mouse kidney (2010)
  • Author(s): Talati G, Ohta A, Rai T, Sohara E, Naito S, Vandewalle A, Sasaki S, Uchida S
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 393 Pages: 844-8
  • Peer Reviewed
• [Journal Article] Generation and analysis of the thiazide-sensitive Na+ -Cl- cotransporter (Ncc/Slc12a3) Ser707X knockin mouse as a model of Gitelman syndrome (2010)
  • Author(s): Yang SS, Lo YF, Yu IS, Lin SW, Chang TH, Hsu YJ, Chao TK, Sytwu HK, Uchida S, Sasaki S, Lin SH
  • Journal Title: Hum. Mutat Volume: 31 Pages: 1304-15
  • Peer Reviewed
• [Journal Article] SPAK-knockout mice manifest Gitelman syndrome and impaired vasoconstriction (2010)
  • Author(s): Yang SS, Lo YF, Wu CC, Lin SW, Yeh CJ, Chu P, Sytwu HK, Uchida S, Sasaki S, Lin SH
  • Journal Title: J. Am. Soc. Nephrol Volume: 21 Pages: 1868-77
  • Peer Reviewed
• [Journal Article] Mechanisms for hypercalciuria in pseudohypoaldosteronism type II-causing WNK4 knock-in mice (2010)
  • Author(s): Yang SS, Hsu YJ, Chiga M, Rai T, Sasaki S, Uchida S, Lin SH
  • Journal Title: Endocrinology Volume: 151 Pages: 1829-36
  • Peer Reviewed
• [Journal Article] Pathophysiological roles of WNK kinases in the kidney (2010)
  • Author(s): Uchida S
  • Journal Title: Pflugers Arch Volume: 460 Pages: 695-702
  • Peer Reviewed
• [Journal Article] Aquaporin-2 regulates cell volume recovery via tropomyosin (2009)
  • Author(s): Li YH, Eto K, Horikawa S, Uchida S, Sasaki S, Li XJ, Noda Y
  • Journal Title: Int. J. Biochem. Cell Biol Volume: 41 Pages: 2466-2476
  • Peer Reviewed
• [Journal Article] Targeted disruption of the Wnk4 genedecreases phosphorylation of Na-Cl cotransporter, increases Na excretion and lowers blood pressure (2009)
  • Author(s): Ohta A, Rai T, Yui N, Chiga M, Yang SS, Lin SH, Sohara E, Sasaki S, Uchida S
  • Journal Title: Hum. Mol. Genet Volume: 18 Pages: 3978-86
  • Peer Reviewed
• [Journal Article] Pancreas-specific aquaporin 12 null mice showed increased susceptibility to caerulein-induced acute pancreatitis (2009)
  • Author(s): Ohta E, Itoh T, Nemoto T, Kumagai J, Ko SB, Ishibashi K, Ohno M, Uchida K, Ohta A, Sohara E, Uchida S, Sasaki S, Rai T
  • Journal Title: Am. J. Physiol. Cell Physiol Volume: 297 Pages: 1368-78
  • Peer Reviewed
• [Journal Article] FAPP2 is required for aquaporin-2 apical sorting attrans-Golgi network in polarized MDCK cells (2009)
  • Author(s): Yui N, Okutsu R, Sohara E, Rai T, OhtaA, Noda Y, Sasaki S, Uchida S
  • Journal Title: Am. J. Physiol. Cell Physiol Volume: 297 Pages: 1389-1396
  • Peer Reviewed
• [Journal Article] Expression and phosphorylation of the Na+-Cl- cotransporter NCC in vivo isregulated by dietary salt, potassium, and SGK1 (2009)
  • Author(s): Vallon V, Schroth J, Lang F, Kuhl D, Uchida S
  • Journal Title: Am. J. Physiol. Renal Physiol Volume: 297 Pages: 704-12
  • Peer Reviewed
• [Journal Article] Function of aquaporin-7 in the kidney and the male reproductive system (2009)
  • Author(s): Sohara E, Uchida S, Sasaki S
  • Journal Title: Handb ExpPharmacol Pages: 219-31
  • Peer Reviewed
• [Journal Article] Dietary salt regulates the phosphorylation of OSR1/SPAK kinases and the sodium chloride cotransporter through aldosterone (2008)
  • Author(s): Chiga M, Rai T, Yang SS, Ohta A, Takizawa T, Sasaki S, Uchida S
  • Journal Title: Kidney Int Volume: 74 Pages: 1403-9
  • Peer Reviewed
• [Journal Article] Intracellular ClC-3 chloride channels promote bone resorption in vitro through organelle acidification in mouse osteoclasts (2008)
  • Author(s): Okamoto F, Kajiya H, Toh K, Uchida S, Yoshikawa M, Sasaki S, Kido MA, Tanaka T, Okabe K
  • Journal Title: Am. J .Physiol. Cell Physiol Volume: 294 Pages: C693-701
  • Peer Reviewed
• [Journal Article] AKAP220 colocalizes with AQP2 in the inner medullary collecting ducts (2008)
  • Author(s): Okutsu R, Rai T, Kikuchi A, Ohno M, Uchida K, Sasaki S, Uchida S
  • Journal Title: Kidney Int Volume: 74 Pages: 1429-33
  • Peer Reviewed
• [Presentation] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates itsubiquitination (2012)
  • Author(s): Hossain Khan MZ, Sohara E, Ohta A, Chiga M, Inoue Y, Isobe K, Wakabayashi M, Naito S, Oi K, Rai T, Sasaki S, Uchida S
  • Organizer: The 45th Annual meeting of American Society of Nephrology
  • Place of Presentation: San Diego (USA)
  • Year and Date: 20121100
• [Presentation] Development of sandwich enzyme linked immunosorbent assay (ELISA) for measurement of urinary total and phosphorylated Na-Clcotransporter (NCC) protein (2012)
  • Author(s): Isobe K, Sohara E, Rai T, Sasaki S,Uchida S
  • Organizer: The 45th Annual meeting of American Society of Nephrology
  • Place of Presentation: San Diego (USA)
  • Year and Date: 20121100
• [Presentation] Chemical library screening for direct SPAK inhibitors by a newly developed ELISA system (2012)
  • Author(s): Kikuchi E, Mori T, Isobe K, Sohara E, Rai T, Sasaki S, Uchida S
  • Organizer: The 45th Annual Meeting of American Society of Nephrology
  • Place of Presentation: San Diego (USA)
  • Year and Date: 20121100
• [Presentation] Generation and analysis of WT-WNK4 transgenic mice revealed the physiological role of WNK4 (2012)
  • Author(s): Mori T, Sohara E, Rai T, Sasaki S, Uchida S
  • Organizer: The 45th Annual Meeting of American Society of Nephrology
  • Place of Presentation: San Diego (USA)
  • Year and Date: 20121100
• [Presentation] Hereditary nephrogenic diabetes insipidus (NDI) in Japanese patients: Analysis of 73 families (2012)
  • Author(s): Sasaki S, Chiga M, Kikuchi E, Uchida S
  • Organizer: The 45th Annual Meeting of American Society of Nephrology
  • Place of Presentation: San Diego (USA)
  • Year and Date: 20121100
• [Presentation] WNK4-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone (2012)
  • Author(s): Susa K, Sohara E, Isobe K, Chiga M, Rai T, Sasaki S, Uchida S
  • Organizer: The 45th Annual Meeting of American Society of Nephrology
  • Place of Presentation: San Diego (USA)
  • Year and Date: 20121100
• [Presentation] Dietary salt intake and angiotensin II regulates WNK-SPAK-NKCC1 phosphorylation cascade in mouse aorta (2012)
  • Author(s): Zeniya M, Sohara E, Oi K, Chiga M, SusaK, Mori T, Rai T, Sasaki S, Uchida S
  • Organizer: The 45th Annual Meeting of American Society of Nephrology
  • Place of Presentation: San Diego (USA)
  • Year and Date: 20121100
• [Presentation] Generation and analyses of AQP11 BAC transgenic mice (2011)
  • Author(s): Inoue Y, Sohara E, Kobayashi K, Rai T,Ishibashi K, Sasaki S, Uchida S
  • Organizer: The 44th Annual Meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20111100
• [Presentation] Development of new systems to measure total and phosphorylated Na-Cl cotransporter(NCC)protein in human urine (2011)
  • Author(s): Isobe K, Ohta A, Sohara E, Rai T, SasakiS, Uchida S
  • Organizer: The 44th Annual Meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20111100
• [Presentation] Urinary excretion of Na-ClCotransporter in exosomes is increased by high salt diet as well as low salt diet (2011)
  • Author(s): Kahn MZH, Sohara E, Ohta A, Naito S, Chiga M, Rai T, Sasaki S, Uchida S
  • Organizer: The 44th Annual Meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20111100
• [Presentation] CFTR is highly expressed in the cyst-lining epithelial cells of the AQP11 knockout mouse kidney (2011)
  • Author(s): Kobayashi K, Uchida S, Sasaki S
  • Organizer: The 44th Annual Meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20111100
• [Presentation] High throughput screening of drugs that inhibit WNK-OSR1/SPAK signaling cascade (2011)
  • Author(s): Mori T, Ohta A, Sohara E, Rai T, Sasaki S, Uchida S
  • Organizer: The 44th Annual Meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20111100
• [Presentation] Increased Na-Cl cotransporter phosphorylation in hyperinsulinemic db/db mice is regulated by insulin/PI3K pathway (2011)
  • Author(s): Nishida H, Sohara E, Alessi DR, Nomura N, Rai T, Sasaki S, Uchida S
  • Organizer: The 44th Annual Meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20111100
• [Presentation] Chemical library screening for drugs to correct intracellular mislocalization of R8L mutant barttin (2011)
  • Author(s): Nomura N, Naito S, Sohara E, Rai T, Sasaki S, Uchida S
  • Organizer: The 44th Annual Meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20111100
• [Presentation] Renal phenotype of WNK3 knockout mouse (2011)
  • Author(s): Oi K, Sohara E, Rai T, Chiga M, Alessi DR, Sasaki S, Uchida S
  • Organizer: The 44th Annual Meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20111100
• [Presentation] Acute insulin stimulation induces phosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney (2011)
  • Author(s): Sohara E, Rai T, Yang SS, Ohta A, Chiga M, Nomura N, Lin SH, Vandewalle A, Sasaki S, Uchida S
  • Organizer: The 44th Annual Meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20111100
• [Presentation] WNK-OSR1/SPAK-SLC12A phosphorylation cascade in the WNK1 (+/-) mice (2011)
  • Author(s): Susa K, Ohta A, Sohara E, Rai T, Kita S, Iwamoto T, Alessi DR, Sasaki S, Uchida S
  • Organizer: The 44th Annual Meeting of American Society ofNephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20111100
• [Presentation] Increased protein abundance of the mutant WNK4 may be a cause of the increased WNK4 kinase activity inthe mouse model of pseudohypoaldosteronism type II (2011)
  • Author(s): Wakabayashi M, Naito S, Sohara E, Rai T, Sasaki S, Uchida S
  • Organizer: The 44th Annual Meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20111100
• [Presentation] Treatment of Bartter syndrome type IV caused by R8L barttin mutation (2010)
  • Author(s): Nomura N, Masato T, Ohta E, Ohta A, Sohara E, Rai T, Sasaki S, Uchida S
  • Organizer: The 43nd Annual Meeting of American Society of Nephrology
  • Place of Presentation: Denver (USA)
  • Year and Date: 20101100
• [Presentation] Investigation of the localization of mouse WNK3 in kidney (2009)
  • Author(s): Oi K, Sohara E, Ohta A, Ohta E, Rai T, Sasaki S, Uchida S
  • Organizer: The 42nd Annual Meeting of American Society of Nephrology
  • Place of Presentation: San Diego (USA)
  • Year and Date: 20091100
• [Presentation] Novel insulin-WNK4-NCC phosphorylation cascade is involved in pathogenesis of PHA II caused by WNK4R1185C mutation (2009)
  • Author(s): Sohara E, Rai T, Ohta A, Ohta E, Sasaki S and Uchida S
  • Organizer: The 42nd Annual Meeting of American Society of Nephrology
  • Place of Presentation: San Diego (USA)
  • Year and Date: 20091100
• [Presentation] Increased phosphorylation of Na-Cl cotransporter (NCC) in the mouse model of pseudohypoaldosteronism type II was dependent on both OSR1 and SPAK kinase activity (2009)
  • Author(s): Chiga M, Ohta A, Rai T, Sohara E, Ohta E, Rafiqi FH, Alessi DR, Sasaki S, Uchida S
  • Organizer: The 42nd Annual Meeting ofAmerican Society of Nephrology
  • Place of Presentation: San Diego(USA)
  • Year and Date: 20091000
• [Presentation] Regulation of WNK1 activation by extracellular ionic conditions (2009)
  • Author(s): Naito S, Ohta A, Ohta E, Sohara E, Rai T, Sasaki S, Uchida S
  • Organizer: The 42nd Annual Meeting of American Society of Nephrology
  • Place of Presentation: San Diego(USA)
  • Year and Date: 20091000
• [Presentation] Generation and analysis of the BsndR8L/R8L knockin mice (2009)
  • Author(s): Nomura N, Masato T, Morimoto T, Kondo Y, Ohta E, Ohta A, Sohara E, Rai T, Sasaki S, Uchida S
  • Organizer: The 42nd Annual Meeting of American Society of Nephrology
  • Place of Presentation: San Diego (USA)
  • Year and Date: 20091000
• [Presentation] Effect of angiotensin II on WNK-OSR1/SPAK-NCC phosphorylation cascade in cultured mpkDCT cells and in vivo mouse kidney (2009)
  • Author(s): Talati G, Ohta A, Rai T, Sohara E, Naito S, Vandewalle A, Sasaki S, Uchida S
  • Organizer: The 42st Annual Meetingof American Society of Nephrology
  • Place of Presentation: SanDiego (USA)
  • Year and Date: 20091000
• [Presentation] Molecular pathogenesis of Bartter syndrome caused by R8L barttin mutation (2009)
  • Author(s): Uchida S, Nomura N, Sugawara N, Morimoto T, Kondo Y, Naito S, Yui N, Oi K, Talat G , KMZ Hossain, Wakabayashi M, Nishida H, Ohta E, Ohta A, Sohara E, Rai T, Sasaki S
  • Organizer: The 40th NIPS International Symposium & Physiology of anion transport and cell volume regulation (PAT-CVR 2009)
  • Place of Presentation: Okazaki
  • Year and Date: 20090800
• [Presentation] (2009)
  • Organizer: The 36th International Congress ofPhysiological Sciences (IUPS2009)
  • Place of Presentation: Kyoto
  • Year and Date: 20090700
• [Presentation] The primary cilia of the AQP11 knockout mouse kidney (2008)
  • Author(s): Kobayashi K, Uchida S, Sasaki S
  • Organizer: The 41st Annual Meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20081100
• [Presentation] Targeted disruption of the Wnk4 gene reduced the phospholylation of OSR1/SPAK and Na-Cl cotransporter in mice (2008)
  • Author(s): Ohta A, Chiga M, Tajima M, Yang SS, Naito S, Rai T, Uchida S, Sasaki S
  • Organizer: The 41st Annual Meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20081100
• [Presentation] Analysis of pancreatic exocrine function ofaquaporin-12 knockout mice (2008)
  • Author(s): Ohta E, Itoh T, Nemoto T, Kumagai J, Ko SBH, Ishibashi K, Ohno M, Uchida K, Ohta A, Rai T, Uchida S, Sasaki S
  • Organizer: The 41st Annual meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20081100
• [Presentation] Basic and Clinical Science Symposium, "Transport regulation by phosphorylation"Phosphorylation-dependent regulation of NCC by WNKs (2008)
  • Author(s): Uchida S
  • Organizer: The 41st Annual meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 20081100
• [Presentation] Epithelial transport: bridges between molecules and function
  • Author(s): Uchida S
  • Organizer: WNK kinases and cation-chloride cotransporters, novel transportsomes regulating blood pressure
• [Remarks] 東京医科歯科大学腎臓内科学
  • URL: http://www.tmd.ac.jp/grad/kid/kid-J.htm