The effect of cancer-targeted liposome with viral proteins on staining and visualizing cancer cells

2010 Fiscal Year Final Research Report

• Project/Area Number: 20249080
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Chiba University
• Principal Investigator: TANZAWA Hideki Chiba University, 大学院・医学研究院, 教授 (50236775)
• Co-Investigator(Kenkyū-buntansha): SHIRASAWA Hiroshi 千葉大学, 大学院・医学研究院, 教授 (00216194) ; SAITO Kengo 千葉大学, 大学院・医学研究院, 助教 (70451755)
• Project Period (FY): 2008 – 2010
• Keywords: 非 / 低病原性ウイルス / シンドビスウイルス / ハイブリッド型リポソーム / 腫瘍特異的吸着性 / 手術支援システム

Research Abstract

We developed a novel fluorescence-labeled cancer-targeted liposome coupled to sindbis virus proteins (SIN hybrid liposome) on the surface that can be used for imaging tumors and optimized for a bio-distribution analysis and an intra-operative navigation system. SIN hybrid liposome was prepared by mixing the pegylated liposome and UV-inactivated Sindbis virus protein that were purified by an ultracentrifuge. The structure of SIN hybrid liposome was observed by a Transmission Electron Microscope. When cancer cells were incubated with SIN hybrid or non-SIN hybrid rhodamin labeled liposomes encapsulating FITC-dextran, both conforcal microscopy and flow cytometry revealed that a large number of rhodamin fluorescence on the cell membrane and FITC in the cytoplasm were detected in the cancer cells treated with SIN hybrid liposomes compared with non-SIN hybrid liposomes. Furthermore, each liposome was systemically injected in tumor bearing mouse model and imaged for distribution using IVIIS lumina systems. SIN hybrid liposome showed distribution and accumulation at the tumor site preferentially and improved the signal-to-noise ration.

Research Products

• [Journal Article] Oncolytic virotherapy for oral squamous cell carcinoma using replication-competent viruses (2009)
  • Author(s): Saito K, Shirasawa H, Isegawa N, Shiiba M, Uzawa K, Tanzawa H, Oral Oncology
  • Journal Title: Oral Oncology 45(12) Pages: 1021-7
  • Peer Reviewed
• [Journal Article] Oncolytic activity of Sindbis virus in human oral squamous carcinoma cells (2009)
  • Author(s): Saito K, Uzawa K, Kasamatsu A, Shinozuka K, Sakuma K, Yamatoji M, Shiiba M, Shino Y, Shirasawa H, Tanzawa H, Br J Cancer
  • Journal Title: Br J Cancer 101(4) Pages: 684-90
  • Peer Reviewed
• [Presentation] The effect of a cisplatin-encapsulating cancer-targeted liposome with viral proteins on cancer cells (2009)
  • Author(s): Saito K., Shirasawa H., Uzawa K., Tanzawa H.
  • Organizer: 68th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Yokohama
  • Year and Date: 2009-10-03
• [Presentation] Oncolytic activity of sindbis virus for oral cancer cells (2008)
  • Author(s): Saito k., Shirasawa H., Shiiba M., Uzawa K., Tanzawa H.
  • Organizer: 3th World Congress on Advances in Oncology and 11th International Symposium on Molecular Medicine
  • Place of Presentation: Greece Krete
  • Year and Date: 2008-10-09
• [Patent(Industrial Property Rights)] 腫瘍特異性を有する新型リポソーム (2010)
  • Inventor(s): 鵜沢一弘、齋藤謙悟、丹沢秀樹、白澤浩、山本惠司、森部久仁一、椎葉正史
  • Industrial Property Rights Holder: 千葉大学
  • Industrial Property Number: 特許,特願2010-086071号
  • Acquisition Date: 2010-04-02