2011 Fiscal Year Final Research Report
Development of an automatic analysis system at the protein-structural mobility using the electron microscope
| Project/Area Number |
20300107
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bioinformatics/Life informatics
|
|---|
| Research Institution | National Institute of Advanced Industrial Science and Technology |
|---|
Principal Investigator |
OGURA Toshihiko 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (70371028)
|
|---|
| Project Period (FY) |
2008 – 2011
|
| Keywords | 電子顕微鏡 / 画像情報処理 / 生物サンプル / 3次元構造 / タンパク質 |
|---|
| Research Abstract |
To reconstruct three-dimensional(3D) structure of proteins, we have developed newly 3D reconstruction algorithm and a high-contrast detection system of the biological samples. Our 3D reconstruction method was composed with the modified Simulated-Annealing algorithm with the high accuracy classification method of the class averages from the protein images by the transmission-electron microscopy. This method enables automatically 3D reconstruction from the protein images. Furthermore, we developed high-contrast and low-damage 3D detection system. This system contains a linear array PD system and new 3D reconstruction method.
|
