• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2010 Fiscal Year Final Research Report

Comprehensive analyses of the axon guidance molecule Draxin

Research Project

  • PDF
Project/Area Number 20300130
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionKumamoto University

Principal Investigator

TANAKA Hideaki  Kumamoto University, 大学院・生命科学研究部, 教授 (90106906)

Co-Investigator(Kenkyū-buntansha) SHINMYO Youhei  熊本大学, 大学院・生命科学研究部, 助教 (00418831)
Project Period (FY) 2008 – 2010
Keywords神経軸索 / ガイダンス / 遺伝子欠損マウス / draxin / 大脳皮質 / genetic interaction / DCC
Research Abstract

The function of the nervous system depends on the establishment of precise and intricate neuronal connections. The combinatorial effects of attractive and repulsive guidance cues, present in the extracellular environment, direct axons to grow towards their intermediate targets and thus play a crucial role in forming proper connections between neurons. Four conserved families of axon guidance cues, the netrin, semaphorin, ephrin and slit proteins, mediate their guidance effects via receptors of the DCC or UNC5, Neuropilin/Plexin, Eph and Robo families, respectively. Although these proteins have been found to regulate a wide variety of guidance decisions, it is expected that others await identification, and will help address the immense complexity of the nervous system. We recently reported the discovery of a novel axon guidance molecule, draxin, which shares no sequence homology with other known guidance molecules (Islam et al., Science 2009). In vitro, draxin can repel spinal commissural axons whose outgrowth is stimulated by netrin-1 ; in vivo, genetic deletion of draxin results in mild guidance defects of spinal commissural axons, and in a dramatic loss of all forebrain commissures by loosing their growing directional cues, but not by their cell death.
Identification of the draxin receptor is important to elucidating the precise mechanism through which it functions. We found unexpectedly that the netrin-receptor DCC binds draxin with high affinity and is required for draxin's inhibitory effect on the cortical neurite outgrowth. This study identifies DCC as a functional receptor or receptor component for draxin. Next, we need to identify what kinds of signaling molecules are activated by draxin and DCC binding.

  • Research Products

    (22 results)

All 2010 2009 2008

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (14 results)

  • [Journal Article] Tsukushi is required for anterior commissure formation in mouse brain.2010

    • Author(s)
      Ito A, Shinmyo Y, Abe T, Oshima N, Tanaka H, Ohta K.
    • Journal Title

      402

      Pages: 813-818

    • Peer Reviewed
  • [Journal Article] Olfactory bulb Axonal outgrowth is inhibited by draxin.2010

    • Author(s)
      Ahmed G, Shinmyo Y, Naser TB, Hossain M, Song X, Tanaka H.
    • Journal Title

      Biochem Biophys Res Commun 398(4)

      Pages: 730-734

    • Peer Reviewed
  • [Journal Article] Draxin is involved in the proper development of the dI3 interneuron in chick spinal cord.2010

    • Author(s)
      Su Y, Zhang S, Islam SM, Shinmyo Y, Naser TB, Ahmed G, Tanaka H.
    • Journal Title

      Developmental Dynamics 239(6)

      Pages: 1654-1663

    • Peer Reviewed
  • [Journal Article] PC3 is involved in the shift from proliferation to differentiation and maturation in spiral ganglion neurons.2010

    • Author(s)
      Hayashida M, Minoda R, Shinmyo Y, Ohta K, Tanaka H, Yumoto E.
    • Journal Title

      Neuroreport Vol.21 No.2

      Pages: 90-93

    • Peer Reviewed
  • [Journal Article] Draxin, a repulsive axon guidance protein, is involved in hippocampal development2010

    • Author(s)
      Zhang S, Su Y, Shinmyo Y, Islam SM, Naser IB, Ahmed G, Tamamaki N, Tanaka H.
    • Journal Title

      Neuroscience Research. Vol.66 No.1.

      Pages: 53-61

    • Peer Reviewed
  • [Journal Article] an axon guidance protein, affects chick trunk neural crest migration.2009

    • Author(s)
      Su Y, Naser IB, Islam SM, Zhang S, Ahmed G, Chen S, Shinmyo Y, Kawakami M, Yamamura K, Tanaka H, Draxin
    • Journal Title

      Development Growth and Differentiation Vol.51. No.9

      Pages: 787-796

    • Peer Reviewed
  • [Journal Article] Analysis of a repulsive axon guidance molecule, draxin, on ventrally directed axon projection in chick early embryonic midbrain.2009

    • Author(s)
      Naser IB, Su Y, Islam SM, Shinmyo Y, Zhang S, Ahmed G, Chen S, Tanaka H.
    • Journal Title

      Developmental Biology Vol.332

      Pages: 351-359

    • Peer Reviewed
  • [Journal Article] Draxin, a Repulsive Guidance Protein for Spinal Cord and Forebrain Commissures.2009

    • Author(s)
      Islam SM, Shinmyo Y, Okafuji T, Su Y, Naser IB, Ahmed G, Zhang S, Chen S, Ohta K, Kiyonari H, Abe T, Tanaka S, Nishinakamura R, Terashima T, Kitamura T, Tanaka H.
    • Journal Title

      Science Vol.323. No.5912

      Pages: 388-393

    • Peer Reviewed
  • [Presentation] Iftekhar B Naser, Draxin, an axon guidance protein, is involved in retinocollicular axon projection.2010

    • Organizer
      第33回日本神経科学学会年会
    • Place of Presentation
      神戸.神戸コンベンションセンター
    • Year and Date
      20100902-20100904
  • [Presentation] Analyses of the development of the spinal nerve in the chick embryo.2010

    • Author(s)
      増田知之
    • Organizer
      第33回日本神経科学学会年会
    • Place of Presentation
      神戸.神戸コンベンションセンター
    • Year and Date
      20100902-20100904
  • [Presentation] Impairment of socio-emotionalbehavior and memory function in Draxin (Dorsal repulsive axon guidance protein) knockout mice.2010

    • Author(s)
      坂本敏郎
    • Organizer
      第33回日本神経科学学会年会
    • Place of Presentation
      神戸.神戸コンベンションセンター
    • Year and Date
      20100902-20100904
  • [Presentation] A Novel repulsive Axon Guidance Protein, Draxin.2010

    • Author(s)
      田中英明
    • Organizer
      8^<th> TLL Life Science Symposium
    • Place of Presentation
      シンガポール.テマセック生命科学研究所
    • Year and Date
      20100201-20100202
  • [Presentation] Correct Wiring of the Neruous System during Development.2010

    • Author(s)
      田中英明
    • Organizer
      BSBMB International
    • Place of Presentation
      バングラディッシュ.ダッカ大学
    • Year and Date
      20100129-20100131
  • [Presentation] Draxin, a novel chemorepulsive axon guidance protein.2009

    • Author(s)
      新明洋平
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋.名古屋国際会議場
    • Year and Date
      20090916-20090918
  • [Presentation] A Novel repulsive Axon Guidance Protein, Draxin.2009

    • Author(s)
      田中英明
    • Organizer
      第52回日本神経化学大会
    • Place of Presentation
      群馬.ホテル天坊
    • Year and Date
      20090621-20090624
  • [Presentation] A Novel repulsive Axon Guidance Protein, Draxin.2009

    • Author(s)
      新明洋平
    • Organizer
      第42回大会日本発生生物学会
    • Place of Presentation
      新潟.朱鷲メッセコンベンションセンター
    • Year and Date
      20090528-20090531
  • [Presentation] Function of draxin on hippocampal development.2008

    • Author(s)
      Sanbing Zhang
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京.東京国際フォーラム
    • Year and Date
      20080709-20080711
  • [Presentation] Functional analysis of draxin in the migration of neural crest cells.2008

    • Author(s)
      Yuhong Su
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京.東京国際フォーラム
    • Year and Date
      20080709-20080711
  • [Presentation] A Novel repulsive Axon Guidance Protein, Draxin.2008

    • Author(s)
      Shahidul. MD.Islam
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京.東京国際フォーラム
    • Year and Date
      20080709-20080711
  • [Presentation] Tsukushiによる網膜幹細胞の未分化性維持機構2008

    • Author(s)
      太田訓正
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京.東京国際フォーラム
    • Year and Date
      20080709-20080711
  • [Presentation] A Novel repulsive Axon Guidance Protein, Draxin.2008

    • Author(s)
      新明洋平
    • Organizer
      日本発生生物学会第41回大会
    • Place of Presentation
      徳島.徳島県郷土文化会館
    • Year and Date
      20080528-20080530
  • [Presentation] Tsukushiは網膜幹/前駆細胞の増殖を制御するWntシグナル阻害因子である2008

    • Author(s)
      太田訓正
    • Organizer
      日本発生生物学会第41回大会
    • Place of Presentation
      徳島.徳島県郷土文化会館
    • Year and Date
      20080528-20080530

URL: 

Published: 2012-01-26   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi