2010 Fiscal Year Final Research Report
Expression Analysis of the Exchangeable Gene Trap Clones by X-gal Staining
| Project/Area Number |
20300146
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory animal science
|
|---|
| Research Institution | Kumamoto University |
|---|
Principal Investigator |
ARAKI Masatake Kumamoto University, 生命資源研究・支援センター, 准教授 (80271609)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ARAKI Kimi 熊本大学, 生命資源研究・支援センター, 准教授 (90211705)
YOSHINOBU Kumiko 熊本大学, 生命資源研究・支援センター, 助教 (20274730)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | リサーチバイオリソース |
|---|
| Research Abstract |
We investigated the expression pattern of the established EGTC mouse lines by X-gal staining. We have analyzed 84 mouse lines, and opened expression patterns of 67 clones. In many case, multiple organs showed strong expression of beta-geo protein. There are almost no relationship between X-gal staining results and TPM (transcripts per million) scores of the EST profile (Unigene). This finding shows the importance of actually monitoring the expression pattern of the reporter gene, when we consider it as promoter resources to produce transgenic mice.
|
