Multi-scale simulation model of cardiomyocyte based on the micromechanical measurement of subcellular structure

2010 Fiscal Year Final Research Report

• Project/Area Number: 20300152
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: The University of Tokyo
• Principal Investigator: SUGIURA Seiryo 東京大学, 大学院・新領域創成科学研究科, 特任教授 (10272551)
• Co-Investigator(Kenkyū-buntansha): HISADA Toshiaki 東京大学, 大学院・新領域創成科学研究科, 教授 (40126149) ; WATANABE Hiroshi 東京大学, 大学院・新領域創成科学研究科, 講師 (10282500) ; YAMASHITA Hiroshi 東京大学, 医学部附属病院, 講師 (50323572)
• Project Period (FY): 2008 – 2010
• Keywords: 心筋細胞 / 微小計測 / 細胞シミュレーション

Research Abstract

In this study, first, we developed novel experimental techniques to evaluate the subcellular structures and properties of ventricular myocytes. Based on these findings, we, then, created a 3D finite element of myocyte in which cardiac electrophysiology, contraction, and metabolism are integrated with detailed subcellular structures. The model successfully reproduced and provided the distribution of ions and metabolites beyond the limit of current experimental observations thus proved to be a useful tool for the study of cardiac pathophysiology.

Research Products

• [Journal Article] Critical role of cardiac t-tubule system for the maintenance of contractile function revealed by a 3D integrated model of cardiomyocytes (2012)
  • Author(s): Hatano A, Okada J, Hisada T, Sugiura S
  • Journal Title: J Biomech Volume: 45 Pages: 815-823
  • Peer Reviewed
• [Journal Article] 心筋細胞のエネルギー代謝におけるミトコンドリアと筋小胞体Ca^<2+>放出チャンネルの相対位置の重要性 (2011)
  • Author(s): 波田野明日可、岡田純一、鷲尾巧、久田俊明、杉浦清了
  • Journal Title: 生体医工学 Volume: Vol.49, No.6 Pages: 829-835
  • Peer Reviewed
• [Journal Article] A 3-D simulation model of cardiomyocyte integrating excitation-contraction coupling and metabolism (2011)
  • Author(s): Hatano A, Okada J, Washio T, Hisada T, Sugiura S
  • Journal Title: Biophys J Volume: 101 Pages: 2601-2610
  • Peer Reviewed
• [Journal Article] Structural heterogeneity in the ventricular wall plays a significant role in the initiation of stretch-induced arrhythmias in perfused rabbit right ventricular tissues and whole heart preparations Circ Res (2010)
  • Author(s): Seo K, Inagaki M, Nishimura S, Hidaka I, Sugimachi M, Hisada T, Sugiura S
  • Volume: 106
  • Pages: 176-184
  • Peer Reviewed
• [Presentation] 心筋細胞内3次元構造を再現した電気生理・エネルギー代謝・力学マルチフィジックスシミュレーション (2011)
  • Author(s): 波田野明日可、岡田純一、鷲尾巧、久田俊明、杉浦清了
  • Organizer: 第50回日本生体医工学会大会
  • Place of Presentation: 東京電機大学
  • Year and Date: 2011-04-30
• [Presentation] Effect of stretch on ventricular arrhythmia (2010)
  • Author(s): SeoK, Inagaki M, Hidaka I, Sugimachi M, Nishimura S, Okada J, Washio T, Watanabe H, Hisada T, Sugiura S
  • Organizer: 5th International Workshop Cardiac mechano-electrical coupling and arrhythmias
  • Place of Presentation: Oxford England
  • Year and Date: 20100901-04
• [Presentation] Ut Heart, a Multi-scale, Multi-physics Simulator (2010)
  • Author(s): Seiryo Sugiura, Takumi Washio, Jun-ichi Okada, Hiroshi Watanabe, Toshiaki Hisada
  • Organizer: 19th International Conference of the Cardiovascular System Dynamics Society
  • Place of Presentation: Fukuoka
  • Year and Date: 2010-09-25
• [Presentation] Juxtaposition of Mitochondria to the Ca2+ release site is crucial for the cardiac energy balance- A 3D simulation of cardiomyocyte (2010)
  • Author(s): Hatano A, Okada J, Washio T, Hisada T, Sugiura S
  • Organizer: 19^<th> International Conference of the Cardiovascular System Dynamics Society
  • Place of Presentation: Fukuoka
  • Year and Date: 2010-09-24
• [Presentation] Micromechanical measurement by laser optical tweezer revealed anisotropy in the stiffness of rat ventricular myocytes (2010)
  • Author(s): Masumoto S, Fukano H, Iwanami S, Yamashita H, Hisada T, Sugiura S
  • Organizer: The 20^<th> World Congress ISHR
  • Place of Presentation: Kyoto
  • Year and Date: 2010-05-16
• [Presentation] Micromechanical measurement by laser optical tweezer revealed anisotropy in the stiffness of rat ventricular myocytes (2009)
  • Author(s): Masumoto S, Fukano H, Iwanami S, Yamashita H, Hisada T, Sugiura S
  • Organizer: The 26^<th> Annual Meeting of International Society for Heart Research Japanese Section
  • Place of Presentation: Sapporo
  • Year and Date: 2009-12-04
• [Presentation] Induction of Ca^<2+> spark by mechanical stress is dependent on the direction of applied stresss in the rat ventricular myocytes (2009)
  • Author(s): Fukano H, Masumoto S, Iwanami S, Yamashita H, Hisada T, Sugiura S
  • Organizer: The 26^<th> Annual Meeting of International Society for Heart Research Japanese Section
  • Place of Presentation: Sapporo
  • Year and Date: 2009-12-04
• [Presentation] Multi-scale approach for the understanding of cardiac function Arrhythmia and muscle contraction(Irisawa Memorial Symposium) (2009)
  • Author(s): Sugiura S, Hatano A, Washio T, Okada J, Watanabe H, Hisada T
  • Organizer: The 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto
  • Year and Date: 2009-07-30
• [Presentation] Inhomogeneous structure of the ventricular wall is a substrate for fatal arrhythmias by modulating the global stretch to create heterogeneous cellular response (2009)
  • Author(s): K Seo, M Inagaki, S Sugiura
  • Organizer: 32nd Annual Meeting of the Japanese Working Group Cardiac Structure and Metabolism
  • Place of Presentation: Osaka
  • Year and Date: 2009-07-04
• [Presentation] Is the contractile dysfunction of detubulated cardiomyocytes caused only by the asynchrony of Ca^<2+> regulation? (2008)
  • Author(s): Fukano H, Seo K, Nishimura S, Yamashita H, Sugiura S
  • Organizer: The 25^<th> Annual Meeting of International Heart Research Japanese Section
  • Place of Presentation: Yokohama
  • Year and Date: 2008-12-05
• [Presentation] Contribution of structural heterogeneity to stretch-induced arrhythmias examined in ventricular tissue and isolated myocytes (2008)
  • Author(s): Seo K, Inagaki M, Nishimura S, Sugimachi M, Hisada T, Sugiura S
  • Organizer: The 25^<th> Annual Meeting of International Heart Research Japanese Section
  • Place of Presentation: Yokohama
  • Year and Date: 2008-12-05
• [Remarks]
  • URL: http://www.sml.k.u-tokyo.ac.jp/