2010 Fiscal Year Final Research Report
Development of high sensitive and high S/N ratio multicolored SNP detection technology toward each locus.
Project/Area Number |
20300183
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Medical systems
|
Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
LEZHAVA Alexander The Institute of Physical and Chemical Research, オミックス機能研究ユニット, ユニットリーダー (40443048)
|
Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Akimitsu 独立行政法人理化学研究所, 岡本独立主幹研究ユニット, ユニットリーダー(独立主幹研究員) (60314233)
|
Project Period (FY) |
2008 – 2010
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Keywords | SmartAmp法 / Exciton Primer / SNPタイピング / Cancer Mutation Detection / Multiplex reaction / End Product Visualization / Warfarin / EGFR |
Research Abstract |
A two-color readout of the Smart Amplification (SmartAmp、SMAP) genotyping system using so-called Exciton Primers have been developed. New method is highly specific and sensitive, permitting a one-step, single-tube mutation detection reaction which delivers a result in 45 minutes directly from blood. Exciton Primers, which are functioning as "fluorescence emission with sequence-specific," after hybridization to complementary sequences, can provide the signals resulting target sequence detection for real-time monitoring of amplification reactions. Applied to the isothermal SmartAmp2 mutation detection process, Exciton Primers show high signal strength with low background leading to a superior specificity and sensitivity compared to SYBR Green I. The genotyping assay can use only one labeled Exciton Primer for endpoint detection, or simultaneously by real-time monitoring detect wild-type and mutant alleles in a one-tube reaction using two Exciton Primers having different dyes. The technique seems very promising for decentralized point-of-care diagnostics since the approach does not involve complicated instrumentation and sample purification. We demonstrated efficiency of the new technology on the example of rapid detection of SNPs and mutations
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Research Products
(15 results)