• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2010 Fiscal Year Final Research Report

A highly sensitive assay system for examining chemical mutagenesity and carcinogenesity using DNA repair-deficient mice

Research Project

  • PDF
Project/Area Number 20310031
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionKyushu University

Principal Investigator

TSUZUKI Teruhisa  Kyushu University, 大学院・医学研究院, 教授 (40155429)

Co-Investigator(Kenkyū-buntansha) NAKATSU Yoshimichi  九州大学, 大学院・医学研究院, 准教授 (00207820)
Project Period (FY) 2008 – 2010
Keywords遺伝子 / 核酸 / 環境 / ゲノム / 放射線 / 突然変異 / 酸化ストレス / 活性酸素
Research Abstract

In order to establish a highly sensitive assay system for examining chemical mutagenesity and tumorigenesity, we performed KBrO_3-induced mutagenesis and tumorigenesis experiments using Ogg1-, Mutyh- or Msh2-deficient mice. We observed an enhanced tumor-formation in the small intestines of Mutyh- or Msh2-deficient mice, as compared with wild type and heterozygous mice. These results indicate that Mutyh- or Msh2-deficient mice are ideal animal models for examining tumorigenesity of chemicals of interest. The number of tumors was marginally increased in Ogg1-deficient mice in which the increased KBrO_3-induced mutagenesis was observed at an almost same extent as in Mutyh-deficient mice, suggesting that the induction of cell death might play a more important role than the suppression of mutagenesis for avoiding intestinal tumorigenesis caused by oxidative stress.

  • Research Products

    (37 results)

All 2011 2010 2009 2008 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (21 results) Book (3 results) Remarks (5 results)

  • [Journal Article] Comprehensive behavioral study of GluR4 knockout mice : implication in cognitive function.2010

    • Author(s)
      N.Sagata, A.Iwaki, T.Aramaki, K.Takao, S.Kura, T.Tsuzuki, R.Kawakami, I.Ito, T.Kitamura, H.Sugiyama, T.Miyakawa, Y.Fukumaki
    • Journal Title

      Genes, Brain Behavior 9

      Pages: 899-909

    • Peer Reviewed
  • [Journal Article] Structural and dynamic features of the MutT protein in the recognition of nucleotides with the mutagenic 8-oxoguanine base2010

    • Author(s)
      T.Nakamura, S.Meshitsuka, S.Kitagawa, N.Abe, J.Yamada, T.Ishino, H.Nakano, T.Tsuzuki, T.Doi, Y.Kobayashi, S.Fujii, M.Sekiguchi, Y.Yamagata
    • Journal Title

      J.Biol.Chem. 285

      Pages: 444-452

    • Peer Reviewed
  • [Journal Article] Targeted sequence alteration of a chromosomal locus in mouse liver.2010

    • Author(s)
      H.Kamiya M.Uchiyam, J.-S.Piao, Y.Nakatsu, T.Tsuzuki, H.Harashima
    • Journal Title

      Inter.J.Pharmaceutics

      Pages: 180-183

    • Peer Reviewed
  • [Journal Article] A novel protein, MAPO1, that functions in apoptosis triggered by O^6-methylguanine mispair in DNA.2009

    • Author(s)
      K.Komori, Y.Takagi, M.Sanada, T.-H.Lim, Y.Nakatsu, T.Tsuzuki, M.Sekiguchi, M.Hidaka.
    • Journal Title

      Oncogene 28

      Pages: 1142-1150

    • Peer Reviewed
  • [Journal Article] Improved gene correction efficiency with a tailed duplex DNA fragment.2008

    • Author(s)
      H.Tsuchiya, M.Uchiyama, K.Hara, Y.Nakatsu, T.Tsuzuki, H.Inoue, H.Harashima, H.Kamiya.
    • Journal Title

      Biochemistry 47

      Pages: 8754-8759

    • Peer Reviewed
  • [Journal Article] Effects of target sequence and sense versus anti-sense strands on gene correction with single-stranded DNA fragments.2008

    • Author(s)
      H.Kamiya, M.Uchiyama, Y.Nakatsu, T.Tsuzuki, H.Harashima.
    • Journal Title

      J.Biochem. 144

      Pages: 431-436

    • Peer Reviewed
  • [Journal Article] DNA repair dysfunction in gasterointestinal tract cancers.2008

    • Author(s)
      Y.Maehara, A.Egashira, E.Oki, K.Kakeji, T.Tsuzuki
    • Journal Title

      Cancer Sci. 99

      Pages: 451-458

    • Peer Reviewed
  • [Journal Article] Oxidative stress-induced tumorigenesis in the small intestine of various types of DNA repair-deficient mice

    • Author(s)
      T.Tsuzuki, J, Piao, T.Isoda, K.Sakumi, Y.Nakabeppu, Y.Nakatsu
    • Journal Title

      Health Physics (in press)

    • Peer Reviewed
  • [Presentation] Oxidative stress-induced tumorigenesis in the small intestine of various types of DNA repair-deficient mice, Workshop : Biological effects of low-level exposure to ionizing radiation2010

    • Author(s)
      T.Tsuzuki, J.Piao, T.Isoda, K.Sakumi, Y.Nakabeppu, Y.Nakatsu
    • Organizer
      health risks and clinical consequences
    • Place of Presentation
      Richland, WA, USA
    • Year and Date
      20100500
  • [Presentation] Prevention of Oxidative Mutangenesis by DNA Repair Enzymes : Implication in Human Cancer2010

    • Author(s)
      T.Tsuzuki, J.Piao, T.Isoda, K.Sakumi, Y.Nakabeppu, Y.Nakatsu
    • Organizer
      2^<nd> Asian Conference on Environmental Mutagens (ACEM) : Harmonize Gene & Environment
    • Place of Presentation
      Pattaya, Thailand
    • Year and Date
      2010-12-17
  • [Presentation] 8-oxoguanineはDNA鎖切断を誘発することで減数分裂期の相同染色体組換え頻度を上昇させる2010

    • Author(s)
      大野みずき, 作見邦彦, 續輝久, 中別府雄作
    • Organizer
      日本生化学会第82回大会・第33回日本分子生物学会年会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
  • [Presentation] 腸管と精巣における放射線誘発DNA損傷とその修復機構の解析2010

    • Author(s)
      中西恵美, 大野みずき, 中津可道, 續輝久
    • Organizer
      日本生化学会第82回大会・第33回日本分子生物学会年会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
  • [Presentation] 酸化損傷DNAが生殖細胞ゲノムに及ぼす影響2010

    • Author(s)
      大野みずき, 中西恵美, 作見邦彦, 古市正人, 中別府雄作, 續輝久
    • Organizer
      日本環境変異学会第39回大会
    • Place of Presentation
      茨城
    • Year and Date
      2010-11-16
  • [Presentation] 低LET放射線による核酸の損傷とその修復機構:腸管と精巣における解析2010

    • Author(s)
      大野みずき, 中西恵美, 中津可道, 續輝久
    • Organizer
      日本放射線影響学会第53大会
    • Place of Presentation
      京都
    • Year and Date
      2010-10-20
  • [Presentation] 酸化ストレス誘発小腸腫瘍に対するDifferentiation-inducing factor-1の抗腫瘍効果2010

    • Author(s)
      高橋富美, 吉原達也, 中津可道, 續輝久, 中別府雄作, 笹栗俊之
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-22
  • [Presentation] 哺乳類細胞のゲノム安定性と細胞死2010

    • Author(s)
      日高真純, 高木康光, Teik-How Lim, 中津可道, 續輝久, 佐野しおり, 坂上竜資, 関口睦夫
    • Organizer
      日本遺伝学会第82回大会
    • Place of Presentation
      札幌
    • Year and Date
      2010-09-20
  • [Presentation] Oxidative stress-induced tumorigenesis in the small intestine of various types of DNA repair-deficient mice2009

    • Author(s)
      T.Tsuzuki, J.Piao, T.Isoda, K.Sakumi, Y.Nakabeppu, Y.Nakatsu
    • Organizer
      The 40th International Symposium of the Princess Takamatsu Cancer Research Fund "Cancer and DNA Repair"
    • Place of Presentation
      Tokyo
    • Year and Date
      2009-11-10
  • [Presentation] 酸化ストレス誘発発がんの抑制に関与する分子機構の解明-Mutyh遺伝子欠損マウスにおける消化管発がんの解析を中心として-2009

    • Author(s)
      續輝久
    • Organizer
      第51回日本消化器病学会大会 シンポジウム:S9炎症と消化器発癌
    • Place of Presentation
      京都
    • Year and Date
      2009-10-15
  • [Presentation] Trp53欠損マウスを用いたKBrO_3投与による酸化ストレス誘発小腸腫瘍の解析2009

    • Author(s)
      松本戴恭, 朴晶淑, 中津可道, 前原喜彦, 續輝久
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-02
  • [Presentation] 酸化ストレス誘発消化管発癌抑制における癌関連遺伝子の働き2009

    • Author(s)
      中津可道, 松本載恭, 朴晶淑, 續輝久
    • Organizer
      日本遺伝学会第81回大会
    • Place of Presentation
      長野
    • Year and Date
      2009-09-16
  • [Presentation] Oxidativestress-induced tumorigenesis in the smallintestine of various types of DNA repair-deficient mice2009

    • Author(s)
      T.Tsuzuki, J.Piao, T.Isoda, K.Sakumi, Y.Nakabeppu, Y.Nakatsu
    • Organizer
      10th International Conference on Environmental Mutagens (ICEM) Symposium : Cancer Models and Mechanisms
    • Place of Presentation
      Italy
    • Year and Date
      2009-08-23
  • [Presentation] Oxidative stress-induced tumorigenesis in the small intestine of various types of DNA repair-deficient mice2009

    • Author(s)
      T.Tsuzuki, J.Piao, T.Isoda, K.Sakumi, Y.Nakabeppu, Y.Nakatsu
    • Organizer
      3rd ASM Conference on DNA Repair and Mutagenesis
    • Place of Presentation
      Canada
    • Year and Date
      2009-06-01
  • [Presentation] Msh2遺伝子欠損マウスにおける酸化ストレス誘発小腸腫瘍の解析2008

    • Author(s)
      朴晶淑, 磯田拓郎, 中津可道, 中別府雄作, 續輝久
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-29
  • [Presentation] 哺乳動物細胞への8-oxo-dGTPの導入はA:T→C:G変異を引き起こす2008

    • Author(s)
      藏忍, 中津可道, 中別府雄作, 續輝久
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-28
  • [Presentation] Prevention of oxidative mutagenesis by MUTYH : Implication in human cancer2008

    • Author(s)
      T.Tsuzuki
    • Organizer
      Environmental Mutagen Society 39th Annual Meeting
    • Place of Presentation
      Puerto Rico, USA
    • Year and Date
      2008-10-21
  • [Presentation] Significance of Error-avoiding Mechanism for Oxidative DNA Damage in Carcinogenesis2008

    • Author(s)
      T.Tsuzuki
    • Organizer
      9th European Biological Inorganic Chemistry Conference
    • Place of Presentation
      Poland
    • Year and Date
      2008-09-05
  • [Presentation] 酸化ストレス誘発発がんの抑制に関与する分子機構の解明-Mutyh遺伝子欠損マウスにおける消化管発がんの解析を中心として2008

    • Author(s)
      續輝久
    • Organizer
      第14回日本家族性腫瘍学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2008-06-20
  • [Presentation] Oxidative Stress-Induced Tumorigenesis in the Small Intestine of Various DNA Repair-Deficient Mice2008

    • Author(s)
      Y.Nakatsu, J.Piao, T.Isoda, K.Sakumi, Y.Nakabeppu, T.Tsuzuki
    • Organizer
      10th International Workshop on Radiation Damage to DNA(IWRDD)
    • Place of Presentation
      福島
    • Year and Date
      2008-06-08
  • [Presentation] DNA修復関連遺伝子欠損マウスにおける酸化ストレス誘発消化管発がん2008

    • Author(s)
      中津可道, 朴晶淑, 磯田拓郎, 續輝久, 作見邦彦, 中別府雄作
    • Organizer
      がん予防大会2008福岡(第9回日本がん分子疫学研究会/第15回日本がん予防学会/第31回日本がん疫学研究会)
    • Place of Presentation
      福岡
    • Year and Date
      2008-05-22
  • [Book] DNA修復異常と発がんモデル(「完全版 マウス・ラット疾患モデル活用ハンドブック」)(秋山徹, 奥山隆平, 河府和義 編)2011

    • Author(s)
      江頭明典, 前原喜彦, 續輝久
    • Total Pages
      82-99
    • Publisher
      羊土社
  • [Book] Oxidative stress -induced tumorigenesis in the small intestines of DNA repair-deficient mice(International Proceedings : The 40th International Symposium of the Princess Takamatsu Cancer Research Fund "DNA Repair and Cancers")(Eds. S.Nishimura, L.A.Loeb, M.Masutani, H.Nakagama, T.Sekiya)2010

    • Author(s)
      T.Tsuzuki, T.Isoda, J.Piao, K.Sakumi, Y.Nakabeppu, Y.Nakatsu
    • Publisher
      Princess Takamatsu Cancer Research Fund
  • [Book] Significance of Error-avoiding Mechanisms for Oxidative DNA Damage in Carcinogenesis(International Proceeding : 9th European Biological Inorganic Chemistry Conference)(Ed. H.Kozlowski)2008

    • Author(s)
      T.Tsuzuki, T.Isoda, J.Piao, K.Yamauchi, A.Egashira, K.Sakamoto, S.Kura, K.Sakumi, Y.Nakabeppu, Y.Nakatsu
    • Publisher
      Medimond
  • [Remarks] ホームページ等

  • [Remarks] 基礎放射線医学(分子遺伝学)分野のURL

    • URL

      http://www.med.kyushu-u.ac.jp/biophys/

  • [Remarks] 九州大学研究者情報のURL

    • URL

      http://hyoka.ofc.kyushu-u.ac.jp/search/index.html

  • [Remarks] 研究代表者:續輝久

    • URL

      http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002228/index.html

  • [Remarks] 研究分担者:中津可道

    • URL

      http://hyoka.ofc.kyushu-u.ac.jp/search/details/K000504/index.html

URL: 

Published: 2012-01-26   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi