Mechanism of Ionizing Radiation Induced Modulation of the Salt Chemotaxis Learning in C. elegans

2011 Fiscal Year Final Research Report

• Project/Area Number: 20310032
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Japan Atomic Energy Agency
• Principal Investigator: SAKASHITA Tetsuya 独立行政法人日本原子力研究開発機構, 量子ビーム応用研究部門, 研究主幹 (30311377)
• Co-Investigator(Kenkyū-buntansha): ISHII Naoaki 東海大学, 医学部, 教授 (60096196) ; YANASE Sumino 大東文化大学, スポーツ健康科学部, 准教授 (90249061)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Michiyo 独立行政法人日本原子力研究開発機構, 量子ビーム応用研究部門, 研究員 (10507437)
• Project Period (FY): 2008 – 2011
• Keywords: 放射線 / 神経科学 / バイオセンサー / システムズ放射線生物学

Research Abstract

We developed a new chemotaxis population assay system with a broad NaCl concentration range from 1 to 100 mM. Using this assay system, we found that odr-3 gene was responsible for ionizing radiation induced modulation of the salt chemotaxis learning. In addition, we estimated the neurotransmission related to the salt chemotaxis learning using the neural network analysis. Moreover, we started the examination for mutants without oxidative stress response genes.

Research Products

• [Journal Article] モデルとしての線虫に観る放射線の生物への影響と研究の展開 (2011)
  • Author(s): 坂下哲哉,鈴木芳代
  • Journal Title: 放射線と産業 Volume: Vol.131 Pages: 37-41
  • URL: http://www.rada.or.jp/radi & indu/hsmaga.html
• [Journal Article] 線虫C. elegansにおける運動と学習を指標とした放射線影響解析 (2011)
  • Author(s): 坂下哲哉,鈴木芳代
  • Journal Title: 放射線生物研究 Volume: Vol.46, No.1 Pages: 30-46
  • URL: http://www.naramed-u.ac.jp/～oncra/rbrc/pub/46-1/46-1matome.doc
• [Journal Article] Radiation biology of Caenorhabditis elegans : germ cell response, aging and behavior (2010)
  • Author(s): Sakashita T, Takanami T, Yanase S, Hamada N, Suzuki M, Kimura T, Kobayashi Y, Ishii N, Higashitani A
  • Journal Title: J Radiat Res Volume: 51(2) Pages: 107-21
  • DOI: DOI:10.1269/jrr.09100
  • Peer Reviewed
• [Journal Article] Effects of ionizing radiation on locomotory behavior and mechanosensation in Caenorhabditis elegans (2009)
  • Author(s): Suzuki M, Sakashita T, Yanase S, Kikuchi M, Ohba H, Higashitani A, Hamada N, Funayama T, Fukamoto K, Tsuji T, Kobayashi Y
  • Journal Title: J Radiat Res Volume: 50(2) Pages: 119-25
  • DOI: DOI:10.1269/jrr.08087
  • Peer Reviewed
• [Journal Article] Hyperoxia exposure induced hormesis decreases mitochondrial superoxide radical levels via Ins/IGF-1 signaling pathway in a long-lived age-1 mutant of Caenorhabditis elegans (2008)
  • Author(s): Yanase S, Ishii N
  • Journal Title: J Radiat Res Volume: 49 Pages: 211-218
  • DOI: DOI:10.1269/jrr.07043
  • Peer Reviewed
• [Journal Article] Locomotion learning behavior relationship in Caenorhabditis elegans following gamma-ray irradiation (2008)
  • Author(s): Sakashita T, Hamada N, Ikeda DD, Suzuki M, Yanase S, Ishii N, Kobayashi Y
  • Journal Title: J Radiat Res Volume: 49(3) Pages: 285-91
  • DOI: DOI:10.1269/jrr.07102
  • Peer Reviewed
• [Presentation] マイクロビームを用いた線虫の化学走性学習に及ぼす放射線影響部位の探索 (2011)
  • Author(s): 坂下哲哉,鈴木芳代,武藤泰子,横田裕一郎,舟山知夫,浜田信行,深本花菜,小林泰彦
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸市
  • Year and Date: 2011-11-17
• [Presentation] 線虫はなぜ放射線に強いか? (2011)
  • Author(s): 石井直明
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸市
  • Year and Date: 2011-11-17
• [Presentation] ins/IGF-1変異体における酸素ラジカル誘発性ホルミシスによる寿命延長機構 (2011)
  • Author(s): 簗瀬澄乃,正山哲嗣,須田斎,石井直明
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸市
  • Year and Date: 2011-11-17
• [Presentation] 線虫の学習行動に対する放射線の影響 (2011)
  • Author(s): 坂下哲哉
  • Organizer: 京都大学放射線生物研究センター第4回ゲノム動態と維持機構の研究会
  • Place of Presentation: 彦根市
  • Year and Date: 2011-07-29
• [Presentation] 線虫の学習機構のニューラルネットワーク解析 (2011)
  • Author(s): 鈴木芳代
  • Organizer: 京都大学放射線生物研究センター第4回ゲノム動態と維持機構の研究会
  • Place of Presentation: 滋賀県彦根市(彦根市)
  • Year and Date: 2011-07-29
• [Presentation] Identification of molecular compensation system of sod-genes expression in nematode C. elegans (2011)
  • Author(s): Yazaki K, Yanase S, Sakamoto T, Nakagawa Y, Ishii N
  • Organizer: 18th International C. elegans Meeting
  • Place of Presentation: ロサンゼルス郡,米国
  • Year and Date: 2011-06-25
• [Presentation] Radiation effects on learning behavior in C. elegans (2011)
  • Author(s): Sakashita T
  • Organizer: The 28th International Symposium on Space Technology and Science
  • Place of Presentation: 那覇市
  • Year and Date: 2011-06-08
• [Presentation] 線虫の学習行動に対する放射線影響とモデル解析の試み (2011)
  • Author(s): 坂下哲哉,鈴木芳代
  • Organizer: 第1回放射線神経生物学研究集会
  • Place of Presentation: 前橋市
  • Year and Date: 2011-01-29
• [Presentation] 内因性ストレス環境下における線虫C. elegansの抗酸化系遺伝子の代替発現機構 (2010)
  • Author(s): 簗瀬澄乃,矢崎孔明,近藤雅紀,石井直明
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-09
• [Presentation] 線虫の学習行動に対する放射線影響とモデル解析の試み (2010)
  • Author(s): 坂下哲哉,鈴木芳代
  • Organizer: 京都大学原子炉実験所専門研究会「ヒト以外への生物への放射線影響」
  • Place of Presentation: 大阪市
  • Year and Date: 2010-11-16
• [Presentation] 線虫C. elegansにおける紫外線感受性株rad-8のエネルギー代謝異常 (2010)
  • Author(s): 安田佳代、須田斎、石井恭正、石井直明
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都市
  • Year and Date: 2010-10-20
• [Presentation] マイクロビームを用いた線虫の化学走性学習に及ぼす放射線影響部位の探索 (2010)
  • Author(s): 坂下哲哉,鈴木芳代,武藤泰子,横田裕一郎,舟山知夫,浜田信行,深本花菜,小林泰彦
  • Organizer: 本宇宙生物科学会第24回大会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-09-17
• [Presentation] Computational inferences on alteration of neurotransmission in chemotaxis learning in Caenorhabditis elegans (2010)
  • Author(s): Suzuki M, Sakashita T, Tsuji T, Kob ayashi Y
  • Organizer: The 20th International Confer ence on Artificial Neural Networks(IC ANN2010)
  • Place of Presentation: テサロニキ市,ギリシャ
  • Year and Date: 2010-09-15
• [Presentation] Some sod genes ; targets of DAF-16 transcription facto r in Caenorhabditis elegans (2010)
  • Author(s): Yanase S, Yazaki K
  • Organizer: Aging, Metabolism, Stress, Pathogenesis and Small RNAs in C. elegans Topic Meeting 2010
  • Place of Presentation: マディソン市,米国
  • Year and Date: 2010-08-03
• [Presentation] CARBON-ION MICROBEAM INDUCES BEHAVIORAL CHANGE IN THE SALT CHEMOTAXIS LEARNING OF C. ELEGANS (2010)
  • Author(s): Sakashita T, Suzuki M, Mutou Y, Yokota Y, Funayama T, Hamada N, Fukamoto K, Kobayashi Y
  • Organizer: 9th International Microbeam Workshop
  • Place of Presentation: ダルムシュタット市,ドイツ
  • Year and Date: 2010-07-16
• [Presentation] MODULATORY EFFECT OF IONIZING RADIATION ON SALT CHEMOTAXIS LEARNING : THE ROLE OF G PROTEIN GAMMA SUBUNIT IN CAENORHABDITIS ELEGANS (2009)
  • Author(s): Hamada N, Sakashita T, Ikeda DD, Yanase S, Suzuki M, Ishii N, Kobayashi Y
  • Organizer: 2nd Asian Congress of Radiation Research
  • Place of Presentation: ソウル市,韓国
  • Year and Date: 20090517-20
• [Presentation] 線虫C. elegansにおける寿命延長を誘発するホルミシス効果は、ミトコンドリア呼吸鎖での酸素消費量およびラジカル産生量の変化に起因する (2009)
  • Author(s): 簗瀬澄乃,石井直明
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島市
  • Year and Date: 2009-11-12
• [Presentation] 線虫の学習行動に与える放射線の影響とマイクロビーム照射実験の構想 (2009)
  • Author(s): 坂下哲哉
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島市
  • Year and Date: 2009-11-01
• [Presentation] SOD-1 deletions in Caenorhabditis elegans alter the localization of intracellular ROS and show molecular compensation (2009)
  • Author(s): Yanase S, Onodera A, Tedesco P, Johnson TE, Ishii N
  • Organizer: 17th International C. elegans Meeting
  • Place of Presentation: ロサンゼルス郡,米国
  • Year and Date: 2009-06-25
• [Presentation] 原子力機構における重イオンマイクロビーム細胞照射装置と生物学研究 (2008)
  • Author(s): 坂下哲哉,舟山知夫,佐藤隆博,浜田信行,深本花菜,横田裕一郎,鈴木芳代,横田渉,神谷富裕,小林泰彦
  • Organizer: 第9回計測自動制御学会システムインテグレーション部門講演会
  • Place of Presentation: 岐阜市
  • Year and Date: 2008-12-06
• [Presentation] Effect of Locally Targeted Carbon-Ion Microbeam Irradiation on Learning Behavior in Caenorhabditis elegans (2008)
  • Author(s): Sakashita T, Suzuki M, Hamada N, Fukamoto K, Yokota Y, Kakizaki T, Wada S, Funayama T, Kobayashi Y
  • Organizer: 8th International Workshop on Microbeam Probes of Cellular Radiation Responses
  • Place of Presentation: 千葉市
  • Year and Date: 2008-11-14
• [Presentation] 線虫の複数の行動に与える高LET放射線の影響 (2008)
  • Author(s): 坂下哲哉,鈴木芳代,浜田信行,深本花菜,横田裕一郎,楚良桜,柿崎竹彦,和田成一,舟山知夫,小林泰彦
  • Organizer: 日本宇宙生物科学会第22回大会
  • Place of Presentation: 奈良市
  • Year and Date: 2008-09-26
• [Presentation] COMPARATIVE STUDY OF SEVERAL BEHAVIORS IN CAENORHABDITIS ELEGANS FOLLOWING HIGH-LET RADIATION EXPOSURE (2008)
  • Author(s): Sakashita T, Suzuki M, Hamada N, Fukamoto K, Yokota Y, Sora S, Kakizaki T, Wada S, Funayama T, Kobayashi Y
  • Organizer: COSPAR2008
  • Place of Presentation: モントリオール市,カナダ
  • Year and Date: 2008-07-17
• [Book] Computational inferences on alteration of neurotransmission in chemotaxis learning in Caenorhabditis elegans (2010)
  • Author(s): Suzuki M, Sakashita T, Tsuji T, Kobayashi Y
  • Total Pages: 291-300
  • Publisher: Springer