Development of phytoremediation system for removal of persistent phenolic pollutants

2010 Fiscal Year Final Research Report

• Project/Area Number: 20310043
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Osaka University
• Principal Investigator: HIRATA Kazumasa Osaka University, 大学院・薬学研究科, 教授 (30199062)
• Co-Investigator(Kenkyū-buntansha): NAKAYAMA Hideki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (30324982) ; KATO Ko 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (80283935) ; HARADA Kazuo 大阪大学, 大学院・薬学研究科, 助教 (50397741)
• Co-Investigator(Renkei-kenkyūsha): MIYASAKA Hitoshi 関西電力株式会社環境技術研究センター, チーフリサーチャー (60451283) ; BAMBA Takeshi 大阪大学, 大学院・工学研究科, 准教授 (10432444)
• Project Period (FY): 2008 – 2010
• Keywords: 環境浄化 / 内分泌かく乱物質 / ペルオキシダーゼ / ポリフェノールオキシダーゼ / 遺伝子組換え植物

Research Abstract

In this study, it was uncovered the following things. i) The key enzymes which detoxicate phenolic endocrine-disrupting chemicals in Portulaca oleracea, that is a promising plant in phytoremediation, are 'polyphenol oxidase' and 'peroxidase'. ii) DNA sequences of these enzymes were decided. iii) These enzymes immobilized onto alginate and glass beads can detoxicate phenolic endocrine-disrupting chemicals under the conditions in which any plant was not able to grow. iv) Salvia cultivars showed a high BPA-eliminating ability under low temperature.

Research Products

• [Journal Article] Molecular cloning and partial characterization of a peroxidases gene expressed in roots of Portulaca oleracea cv. that is potentially useful for the remediation of phenolic pollutants (2011)
  • Author(s): Takeshi Matsui, Yuki Nomura, Mai Takano, Sofue Imai, Hideki Nakayama, Hitoshi Miyasaka, Hitoshi Okuhata, Satoshi Tanaka, Hideyuki Matsuura, Kazuo Harada, Takeshi Bamba, Kazumasa Hirata, Ko Kato
  • Journal Title: Bioscience, Biotechnology, and Biochemistry in press
  • Peer Reviewed
• [Journal Article] Floricultural Salvia plants have a high ability to eliminate bisphenol A (2010)
  • Author(s): Hiroshi Okuhata, Kazunori Ikeda, Hitoshi Miyasaka, Satoru Takahashi, Takeshi Matsui, Hideki Nakayama, Ko Kato, Kazumasa Hirata
  • Journal Title: Journal of Bioscience and Bioengineering 110 Pages: 99-101,Doi:10.1016/j.jbiosc.2009.12.014
  • Peer Reviewed
• [Journal Article] 環境ホルモンを分解する植物-環境浄花- (2009)
  • Author(s): 平田収正、原田和生、奥畑博史、宮坂均
  • Journal Title: Bio Industry 11 Pages: 15-20
  • Peer Reviewed
• [Presentation] 園芸植物Portulaca oleracea 由来ポリフェノールオキシダーゼの遺伝子単離と内分泌撹乱物質代謝能の解析 (2010)
  • Author(s): 金田洋和、松井健史、富安諒介、渡辺一平、黒田友佳子、松浦秀幸、原田和生、奥畑博史、仲山英樹、宮坂均、加藤晃、平田収正
  • Organizer: 第28回日本植物細胞分子生物学会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-09-02
• [Presentation] 園芸植物Portulaca oleracea由来BPA代謝酵素の固定化 (2010)
  • Author(s): 金田洋和
  • Organizer: 平成22年度生物工学若手研究者の集い
  • Place of Presentation: 岡山県倉敷シーサイドホテル
  • Year and Date: 2010-07-03
• [Presentation] Portulaca oleraceaによる内分泌撹乱物質の浄化 (2009)
  • Author(s): 黒田友佳子
  • Organizer: 第4回質量分析夏の学校
  • Place of Presentation: 大阪府長居ユースホステル
  • Year and Date: 2009-09-18