2012 Fiscal Year Final Research Report
In-Situ Single-Molecule Fluorescence Imaging of Membrane Protein Interaction Using Nano-Structured Glass Plate
| Project/Area Number |
20310069
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Waseda University |
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Principal Investigator |
TANII Takashi 早稲田大学, 理工学術院, 教授 (20339708)
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| Project Period (FY) |
2008 – 2012
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| Keywords | 1分子イメージング / ナノ計測 |
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| Research Abstract |
For in-situ single-molecule fluorescence imaging of membrane proteins, we investigated the structure of nano-waveguides using computational optics simulation, followed by fabrication of the waveguides on glass substrates. We studied the feasibility of the waveguides for real-time single-molecule imaging in relation to the waveguide structure. We have developped processes for the fabrication of both etched waveguides and planer waveguides. We showed that the protein-protein interaction can be fluorescently detected with the etched waveguide, and also that the signal-to-noise ratio of the etched waveguide is six times the conventional waveguide. Although we have not yet succeeded in observing menbrain protein interaction using the palner waveguide, we constructed the elemental techniques for the observation, and have succeeded in detecting the single-molecule fluorescence using the planer waveguide.
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Research Products
(22 results)
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[Journal Article] Green Tea Epigalocatechin Gallate (EGCG) Exhibits Anticancer Effect in Human Pancreatic Carcinoma Cells via Inhibition of both Focal Adhesion Kinase and Insulin-like Growth Factor-I Receptor2010
Author(s)
H.A.Vu, Y.Beppu, H.T.Chi, K.Sasaki, H.Yamamoto, P.T.Xinh, T.Tanii, Y.Hara, T.Watanabe, Y.Sato, I.Ohdomari
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Journal Title
Journal of Biomedicine and Biotechnology
Pages: 290516
DOI
Peer Reviewed
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[Journal Article] Effect of the C-Terminal Truncation on the functional Cycle of Chaperonin GroEL: Implication that the C-Terminal Region Faciliates the Transaction from the Folding-Arrested to the Folding-Competent State2008
Author(s)
M.Suzuki, T.Ueno, R.Iizuka, T.Miura, T.Zako, R.Akahori, T.Miyake, N.Shimamoto, M.Aoki, T.Tanii, I.Ohdomari, T.Funatsu
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Journal Title
The Journal of Biological Chemistry
Volume: 283
Pages: 23931
DOI
Peer Reviewed
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