Development of Microdevices for Cell Assay

2010 Fiscal Year Final Research Report

• Project/Area Number: 20310072
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Microdevices/Nanodevices
• Research Institution: Nagoya University
• Principal Investigator: TOKESHI Manabu Nagoya University, 工学研究科, 准教授 (60311437)
• Co-Investigator(Renkei-kenkyūsha): BABA Yoshinobu 名古屋大学, 大学院・工学研究科, 教授 (30183916) ; KAJI Noritada 名古屋大学, 大学院・工学研究科, 助教 (90402479) ; CHAN Yu 名古屋大学, 大学院・工学研究科, 研究員 (40467329)
• Project Period (FY): 2008 – 2010
• Keywords: マイクロ化学チップ / 細胞アッセイ / リアルタイムPCR

Research Abstract

We developed a micro device that a series of bioassay from the cell culture to the gene expression analysis is possible. Two kinds of devices were developed : a device that enables circumfusion culture of suspension cells (yeast) while exchanging media, and a genetic analysis device that enables real-time PCR. An integrated device of these two devices was successfully developed.

Research Products

• [Journal Article] 電気学会論文誌E (2010)
  • Author(s): 杉浦佳奈子、加地範匡、岡本行広、渡慶次学、馬場嘉信
  • Journal Title: 130巻 Pages: 471-475
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Hiroshi Yukawa, Yukimasa Kagami, Masaki Watanabe, Koichi Oishi, Yoshitake Miyamoto, Yukihiro Okamoto, Manabu Tokeshi Noritada Kaji, Hirofumi Noguchi, Kenji Ono, Makoto Sawada, Yoshinobu Baba, Nobuyuki Hamajima, Shuji Hayashi
  • Journal Title: Biomaterials 31 Pages: 4094-4103
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Kanako Sugiura, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
  • Journal Title: Proc.μ-TAS 2009 2 Pages: 1243-1245
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Toyohiro Naito, Ai Yatsuhashi, Noritada Kaji, Taeko Ando, Kazuo Sato, Hisao Moriya, Hiroaki Kitano, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
  • Journal Title: Paoc.μ-TAS 2009 1 Pages: 627-629
  • Peer Reviewed
• [Presentation] (2011)
  • Author(s): 内藤豊裕、加地範匡、岡本行広、渡慶次学、馬場嘉信
  • Organizer: 日本化学会第91春季年会(要旨集)
  • Year and Date: 2011-03-11
• [Presentation] International Symposium : Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules(ISSMA 2011) (2011)
  • Author(s): Toyohiro Naito, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
  • Place of Presentation: Kyoto, Japan.
  • Year and Date: 2011-01-24
• [Presentation] The International Chemical Congress of Pacific Basin Societies(Pacifichem 2010) (2010)
  • Author(s): Toyohiro Naito, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
  • Place of Presentation: Hawaii, USA.
  • Year and Date: 2010-12-16
• [Presentation] 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS 2009) (2009)
  • Author(s): Kanoko Sugiura, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
  • Place of Presentation: Jeju, Korea.
  • Year and Date: 2009-10-03
• [Presentation] 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS 2009) (2009)
  • Author(s): Toyohiro Naito, Ai Yatsuhashi, Noritada Kaji, Taeko Ando, Kazuo Sato, Hisao Moriya, Hiroaki Kitano, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
  • Place of Presentation: Jeiu, Korea.
  • Year and Date: 2009-10-02
• [Presentation] (2009)
  • Author(s): 八橋愛、内藤豊裕、加地範匡、岡本行宏、渡慶次学、馬場嘉信
  • Organizer: 第19回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 広島大学
  • Year and Date: 2009-05-29
• [Remarks] ホームページ等
  • URL: http://www.apehem.nagoya-u.ac.jp/III-2/baba-ken/