2010 Fiscal Year Final Research Report
Strategies to search functional RNAs using yeast as a model system
| Project/Area Number |
20310114
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
基礎ゲノム科学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ITO Takashi 東京大学, 大学院・理学系研究科, 教授 (90201326)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 非コードRNA |
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| Research Abstract |
It is well documented that eukaryotic genome is pervasively transcribed to generate numerous novel non-coding RNAs, but the significance of such transcription remains controversial. We found that yeast cell transcribes a bacterial genome maintained as an artificial chromosome, which should have no biological function, as efficiently as its natural chromosomes. We also showed that limiting transcriptional capacity of yeast leads to a preferential decrease in the level of novel non-coding RNA species identified in recent studies. These results do not contradict with the idea that pervasive transcription is largely functionless.
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Research Products
(5 results)
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[Journal Article] Translation initiation : a regulatory rolefor poly(A) tracts in front of the AUG codon in Saccharomyces cerevisiae2011
Author(s)
Xia, X., MacKay, V., Yao, X., Wu, J., Miura, F., Ito, T., Morris, D. R.
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Journal Title
Genetics
Volume: 189
Pages: 469-478
DOI
Peer Reviewed
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[Journal Article] Absolute quantification of budding yeasttranscriptome by means of competitive PCRbetween genomic and complementary DNAs2008
Author(s)
Miura, F., Kawaguchi, N., Yoshida, M., Uematsu, C., Kito, K., Sakaki, Y., Ito, T.
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Journal Title
BMC Genomics
Volume: 9
Pages: 574
DOI
Peer Reviewed
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