2010 Fiscal Year Final Research Report
Comprehensive analysis of protein turnover
| Project/Area Number |
20310117
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
基礎ゲノム科学
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
NAKAHIGASHI Kenji Keio University, 政策・メディア研究科, 准教授 (70322740)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ISHIHAMA YASUSHI 京都大学, 大学院・薬学研究科, 教授 (30439244)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | ゲノム発現 / タンパク質ターンオーバー / 翻訳 / タンパク質分解 |
|---|
| Research Abstract |
We have developed methods to comprehensively quantify cellular protein turnover, including synthesis rate, degradation rate and translation efficiency of corresponding mRNA. Using these methods we quantified turnover of more than 1500 protein in E. coli, and found that most of the protein, excluding about 5% of unstable protein, were quite stable without any degradation. Upon transition from anaerobic to aerobic growth condition, levels of metabolic enzymes changes dramatically. However these changes are brought mostly by changes in synthesis rate but no apparent degradation of unwanted enzymes in central carbon metabolism were observed.
|
-
-
-
-
[Journal Article] Update on the Keio collection of Escherichia coli single-gene deletion mutants2009
Author(s)
Yamamoto N, Nakahigashi K, Nakamichi T, Yoshino M, Takai Y, Touda Y, Furubayashi A, Kinjyo S, Dose H, Hasegawa M, Datsenko KA, Nakayashiki T, Tomita M, Wanner BL, Mori H
-
Journal Title
Mol Syst Biol 5
Pages: 335
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
