2010 Fiscal Year Final Research Report
Analysis of molecular assemblage in lipid bilayers using bicelles
| Project/Area Number |
20310132
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Osaka University |
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Principal Investigator |
MATSUMORI Nobuaki Osaka University, 大学院・理学研究科, 准教授 (50314357)
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| Co-Investigator(Kenkyū-buntansha) |
MURATA Michio 大阪大学, 大学院・理学研究科, 教授 (40183652)
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| Project Period (FY) |
2008 – 2010
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| Keywords | バイセル / 脂質二重膜 / 脂質ラフト / スフィンゴミエリン / アンフィジノール / 動的光散乱 / 立体配座 |
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| Research Abstract |
Bicelles are lipid aggregates composed of long-chain phospholipid and short-chain one. Since bicelles have true bilayer portions, they are used as a membrane model. In this study, we developed a methodology to analyze the structure of membrane associated bioactive compounds using bicelles. We first applied the methodology to antifungal compound amphidinol, and successfully determined its structure in membrane and proposed the mechanism of its biological activity. Then we analyzed the structure of sphingomyelin, which draws a lot of attention as a component of lipid rafts, and revealed molecular basis of raft formation.
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[Journal Article] Identification of Ras Superfamily Proteins as Yessotoxin Binding Target from Blood Cell Membranes.2010
Author(s)
S. Ujihara, T. Oishi, R. Mouri, R. Tamate, K. Konoki, N. Matsumori, M. Murata, Y. Oshima, N. Sugiyama, M. Tomita, Y. Ishihama
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Journal Title
Bioorg.Med.Chem.Lett. 20
Pages: 6643-6646
Peer Reviewed
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