2010 Fiscal Year Final Research Report
Principle of replication machinery for archaeal DNA replication
| Project/Area Number |
20310134
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Living organism molecular science
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
ISHINO Yoshizumi Kyushu University, 大学院・農学研究院, 教授 (30346837)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ATOMI Haruyuki 京都大学, 工学研究科, 教授 (90243047)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | DNA replication / 超好熱性アーキア / DNA複製 / DNA修復 / 生体高分子 / レプリソーム |
|---|
| Research Abstract |
We characterized three Mcm and two PCNA proteins from Thermococcus kodakaraensis. One of the three mcm genes and one of the two pcna genes seemed to be essential for the life of T. kodakaraensis. Furthermore, we detected the functions of Mcm and PCNA proteins in vitro. In addition, we made 3D structure models of PCNA-DNA ligase-DNA, PCNA-DNA polymerase-DNA, and PCNA-UDG-AP-DNA, and discussed their structure-function relationships.
|
Research Products
(23 results)
