2010 Fiscal Year Final Research Report
Generation of a New Class of Catalytic Antibodies based Directed Evolution
| Project/Area Number |
20310138
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Living organism molecular science
|
|---|
| Research Institution | Osaka Prefecture University |
|---|
Principal Investigator |
IKUO Fujii Osaka Prefecture University, 理学系研究科, 教授 (70189984)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TSUMURAYA Takeshi 大阪府立大学, 理学系研究科, 准教授 (00372855)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | 抗体酵素 / モノクローナル抗体 / 免疫学 / タンパク質 |
|---|
| Research Abstract |
In this study we have tried to generate a new class of catalytic antibodies by two ways, directed evolution and newly designed hapten immunization. We have developed a yeast surface-displayed library of catalytic antibodies showing a luciferase-like activity. In addition, we demonstrate a single antibody catalyzing multiple chemical transformations by the generation of antigen-combining site that function as an apoprotein for binding functionalized small non-protein components. Replacement of this cofactor with acidic and amino cofactors enabled the antibodies to catalyze β-elimination, decarboxylation and aldol reactions with large rate accelerations.
|
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Critical Contribution of Aromatic Rings to Specific Recognition of Polyether Rings by Antibody : The Case of Ciguatoxin CTX3C and Its Specific Antibody 1C92008
Author(s)
K. Tsumoto, A. Yokota, Y. Tanaka, M. Ui, T. Tsumuraya, I. Fujii, I. Kumagai, Y. Nagumo, H. Oguri, M. Inoue, M. Hirama
-
Journal Title
J.Mol.Biol. 283
Pages: 12259-12266
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
