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2010 Fiscal Year Final Research Report

Molecular basis of the splicing regulators that are related to the diseases.

Research Project

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Project/Area Number 20310141
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

MUTO Yutaka  The Institute of Physical and Chemical Research, RNA生物学研究チーム, チームリーダー (30192769)

Project Period (FY) 2008 – 2010
Keywordsスプライシング / 前頭側頭型痴呆症 / 筋強直性ジストロフィー / RNA結合蛋白質 / CUG-BP1 / MBNL / Tra2β / NMR
Research Abstract

In eukaryotic cells, the pre-mRNA splicing reaction plays a crucial role in the regulation of the biological systems. In the present study, we have investigated the protein-protein and/or protein-RNA interactions of the splicing constitutive (U1/U2 snRNP) and the regulatory factors (especially those related to DM and FTD) on the structural point of view.

  • Research Products

    (27 results)

All 2011 2010 2009 2008

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (14 results) Book (1 results)

  • [Journal Article] Structures of the first and second double-stranded RNA-binding domains of human TAR RNA-binding protein2011

    • Author(s)
      Yamashita S., Nagata T., Kawazo M., Takemoto C., Kigawa T., Guntert P., Kobayashi N., Terada T., Shirouzu M., Wakiyama M., Muto Y., Yokoyama S.
    • Journal Title

      Protein Science 20

      Pages: 118-130

    • Peer Reviewed
  • [Journal Article] Structural basis for the dual RNA-recogniton mode of hman Ta2-β RRM2011

    • Author(s)
      Tsuda K., Someya T., Kuwasako K., Takahashi M., He F., Unzai S., Inoue M., Harada T., Watanabe S., Terada T., Kobayashi N. Shirouzu M., Kigawa T., Tanaka A., Sugano S., Guntert P., Yokoyama S., Muto Y.
    • Journal Title

      Nucleic Acids Res 39

      Pages: 1538-1553

    • Peer Reviewed
  • [Journal Article] Isoforms of U1-70K control subunit dynamics in the human spliceosomal U1 snRNP.2009

    • Author(s)
      Hernadez H., Makorova OV., Makorova EM., Morqner N., Muto Y., Krummel DP., Robinson CV.
    • Journal Title

      Plos One 4

      Pages: e7202

    • Peer Reviewed
  • [Journal Article] Structural basis for the sequence-specific RNA-recognition mechanism of human CUG-BP1 RRM32009

    • Author(s)
      Tsuda K, Kuwasako K, Takahashi M, Someya T, Inoue M, Terada T, Kobayashi N, Shirouzu M, Kigawa T, Tanaka A, Sugano S, Guntert P, Muto Y, Yokoyama S.
    • Journal Title

      Nucleic Acids Res. 37

      Pages: 5151-5155

    • Peer Reviewed
  • [Journal Article] Characterization of a heat-stable enzyme possessing GTP-dependent RNA ligase activity from a hyperthermhilic archaeon2009

    • Author(s)
      Kanai A., Sato A., Fukuda Y., Okada K., Matsuda T., Sakamoto T., Muto Y., Yokoyama S., Kawai G., Tomita M.
    • Journal Title

      Pyrococcus furiosus.RNA 15

      Pages: 420-431

    • Peer Reviewed
  • [Journal Article] Solution structure of the RNA binding domain in the human muscleblind-like protein 2.2009

    • Author(s)
      He F., Dang W., Abe C., Tsuda K., Inoue M., Watanabe S., Kobayashi N., Kigawa T., Matsuda T., Yabuki T., Aoki M., Seki E., Harada T., Tomabechi Y., Terada T., Shirouzu M., Tanaka A., Guntert P., Muto Y.,, Yokohama S.
    • Journal Title

      Protein Science 18

      Pages: 81-90

    • Peer Reviewed
  • [Journal Article] AU-rich RNA-binding induces changes in the quaternary structure of AUH2009

    • Author(s)
      Kurimoto K., Kuwasako K., Sandercock AM. Unzai S., Robinson, CV. Muto Y., Yokohama S.
    • Journal Title

      Proteins 75

      Pages: 360-72

    • Peer Reviewed
  • [Journal Article] Solution Structure of the GUCT Domain from Human RNA Helicase II/Guβ Reveals the RRM Fold, but Implausible RNA Interactions2009

    • Author(s)
      Ohnishi S., Paakkonen K., Koshiba S., Tochio N., Sato M., Kobayashi N., Harada T., Watanabe S., Muto Y., Guntert P., Tanaka A., Kigawa T., Yokoyama S.
    • Journal Title

      Proteins 74

      Pages: 133-144

    • Peer Reviewed
  • [Journal Article] The RRM domain of poly(A)-specific ribonuclease has a noncanonical binding site for mRNA cap analog recognition.2008

    • Author(s)
      Nagata T., Suzuki S., Endo R., Shirouzu M., Terada T., Inoue M., Kigawa T., Kobayashi N., Guntert P., Tanaka A., Hayashizaki Y., Muto Y., Yokoyama S.
    • Journal Title

      Nucleic Acids Res. 36

      Pages: 4754-4767

    • Peer Reviewed
  • [Journal Article] Solution structure of the second RNA recognition motif (RRM) domain of murine T cell intracellular antigen-1 (TIA-1) and its RNA recognition mode.2008

    • Author(s)
      Kuwasako K., Takahashi M., Tochio N., Abe C., Tsuda K., Inoue M., Terada T., Shirouzu M., Kobayashi N., Kigawa T., Taguchi S., Tanaka A., Hayashizaki Y., Guntert P., Muto Y., Yokoyama S.
    • Journal Title

      Biochemistry. 47

      Pages: 64

    • Peer Reviewed
  • [Journal Article] Crystal structure of archaeal tRNA (m1G37) methyltransferase aTrm52008

    • Author(s)
      Goto-Ito S., Ito T., Ishii R., Muto Y., Bessho Y., Yokoyama S.
    • Journal Title

      Proteins 72

      Pages: 1274-1289

    • Peer Reviewed
  • [Journal Article] Complex assembly mechanism and an RNA-bindig mode of the human p1-SF3b155 spliceosomal protein complex identified by NMR solution structure and functional analyses.2008

    • Author(s)
      Kuwasako K., Dohmae N., Inoue M., Shirouzu M., Taguchi S., Guntert P., Seraphin B., Muto Y., Yokoyama S.
    • Journal Title

      Proteins 71

      Pages: 1617-1636

    • Peer Reviewed
  • [Presentation] RNAの高次構造に依存したhTra2-βのRNA認識メカニズムの解明2010

    • Author(s)
      津田健吾、桑迫香奈子、高橋真梨、染谷龍彦、白水美香子、寺田貴帆、井上真、木川隆則、菅野純夫、横山茂之、武藤裕
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会(BMB2010)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
  • [Presentation] Solution Structure of Splicing Factor Motif (SFM) in Prp18 from Homo sapiens2010

    • Author(s)
      He F., Inoue M., Kigawa T., Takahashi M., Kuwasako K., Tsuda K., Kobayashi N., Terada T., Shirouzu M., Tanaka A., Sugano S., Guntert P., Yokoyama S., Muto Y.
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会(BMB2010)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
  • [Presentation] Structural basis for the sequence-specific RNA-recognition mechanism of Bruno-like family proteins2010

    • Author(s)
      Muto Y.
    • Organizer
      The 19th CDB Meeting : RNA Sciences in Cell and Developmental Biolgy
    • Place of Presentation
      Kobe
    • Year and Date
      2010-05-10
  • [Presentation] AU-rich RNA-binding induces changes in the quaternary structure of AUH2009

    • Author(s)
      栗本一基、桑迫香奈子、Alan M.Sandercock,雲財悟、Carol V.Robinson,武藤裕、横山茂之
    • Organizer
      2009年度日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-12
  • [Presentation] eIF4BとeIF4HのRNAに対する結合能の違い2009

    • Author(s)
      津田健吾、鈴木咲良、桑迫香奈子、井上真、白水美香子、寺田貴帆、木川隆則、武藤裕、菅野純夫、横山茂之
    • Organizer
      2009年度日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-12
  • [Presentation] miRNAの抑制に関わる蛋白質の構造解析2009

    • Author(s)
      高橋真梨、染谷龍彦、井上真、寺田貴帆、白水美香子、渡部暁、原田拓志、桑迫香奈子、津田健吾、河合剛太、坂本泰一、木川隆則、菅野純夫、武藤裕、横山茂之
    • Organizer
      2009年度日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-12
  • [Presentation] Structural and functional characterization of the NHR1 domain of the Drosophila Neuralized E3 ligase in the Notch signaling pathway2009

    • Author(s)
      何発虎、斎藤講平、小林直宏、原田拓志、渡部暁、木川隆則、Peter Guntert,小原収、田仲昭子、雲財悟、武藤裕、横山茂之
    • Organizer
      2009年度日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-11
  • [Presentation] RNAi関連因子hTRABP2の構造生物学的解析2009

    • Author(s)
      山下征輔、川添将仁、竹本千重、赤坂領吾、永田崇、武藤裕、木川隆則、滝元宏治、脇山素明、横山茂之
    • Organizer
      2009年度日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
  • [Presentation] 構造生物学的研究によって明らかにされる"RNA recognition motif (RRM)"の多様性2009

    • Author(s)
      桑迫香奈子、菅野純夫、林崎良英、木川隆則、武藤裕、横山茂之
    • Organizer
      RNAフロンティアミーティング2009
    • Place of Presentation
      葉山
    • Year and Date
      2009-09-26
  • [Presentation] RNA recognition motifドメインにおける分子認識の違い2008

    • Author(s)
      津田健吾、桑迫香奈子、高橋真梨、染谷龍彦、武藤裕、白水美香子、寺田貴帆、井上真、木川隆則、菅野純夫、横山茂之
    • Organizer
      2008年度日本分子生物学会・生化学会 合同大会
    • Year and Date
      2008-12-09
  • [Presentation] MBNL2蛋白質に見られるCCCH-type Zn fingerドメインの新規溶液構造2008

    • Author(s)
      何発虎、党偉栄、阿部千景、津田健吾、井上真、木川隆則、松田貴意、矢吹孝、青木雅昭、関英子、寺田貴帆、白水美香子、田仲昭子、菅野純夫、武藤裕、横山茂之
    • Organizer
      2008年度日本分子生物学会・生化学会 合同大会
    • Year and Date
      2008-12-09
  • [Presentation] 選択的スプライシングを調節するTIA-1 RRM2の立体構造解析およびRNA認識2008

    • Author(s)
      高橋真梨、桑迫香奈子、栃尾尚哉、阿部千景、津田健吾、井上 真、寺田貴帆、白水美香子、小林直宏、木川隆則、田口精一、田仲昭子、林崎良英、Peter Guntert,武藤裕、横山茂之
    • Organizer
      2008年度日本分子生物学会・生化学会 合同大会
    • Year and Date
      2008-12-09
  • [Presentation] Complex assembly mechanism and an RNA-binding mode of the human p14-SF3b155 spliceosomal protein complex identified by NMR solution structure and functional analysis2008

    • Author(s)
      Kanako Kuwasako, Naoshi Dhomae, Mio Inoue, Mikako Shirouzu, Seiichi Taguchi, Peter Guntert, Bertrand Seraphin, Yutaka Muto, Shigeyuki Yokoyama
    • Organizer
      2008年度日本分子生物学会・生化学会 合同大会
    • Year and Date
      2008-12-09
  • [Presentation] Structural Studies of the RRM : mRNA 5' cap complex2008

    • Author(s)
      Nagata T., Suzuki S., Endo R., Shirouzu M., Terada T., Inoue M., Kigawa T., Guntert P., Hayashizaki Y., Muto Y., Yokoyama S.
    • Organizer
      XXIII International Conference on Magnetic Resonance in Biological Systems
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2008-08-28
  • [Book] 生体の科学(59巻5号 分子遺伝学・遺伝子・遺伝子工学 miRNA)2008

    • Author(s)
      武藤裕

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Published: 2012-01-26   Modified: 2016-04-21  

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