2010 Fiscal Year Final Research Report
Study on the fluctuation of biomolecules around the thermal equilibriumpoint by means of single molecule measurements.
Project/Area Number |
20350009
|
Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Physical chemistry
|
Research Institution | Osaka University |
Principal Investigator |
MIYASAKA Hiroshi Osaka University, 大学院・基礎工学研究科, 教授 (40182000)
|
Project Period (FY) |
2008 – 2010
|
Keywords | 単一分子計測 / 電子移動 / DNA / 構造揺らぎ / 光子統計 |
Research Abstract |
Apparatus that can measure dynamic behaviors of a single molecule in solution phase was constructed by combining an optical microscope, detectors of the fluorescence, and laser light sources. This apparatus was applied to the elucidation of conformational changes of DNA in water in sub nanosecond to millisecond range. The analysis of photon bunching and anti-bunching behaviors of the fluorescence from a moiety connected with DNA led to the conclusion that the faster conformational rearrangement occurring in several hundreds of ns was followed by the slower one with several-several tens μs time region.
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Research Products
(12 results)