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2011 Fiscal Year Final Research Report

Versatile Molecular Devices Based on Functional DNA and PNA Complexes

Research Project

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Project/Area Number 20350038
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionKumamoto University

Principal Investigator

IHARA Toshihiro  熊本大学, 大学院・自然科学研究科, 教授 (40253489)

Co-Investigator(Kenkyū-buntansha) SAKURAI Toshihiko  鳥取大学, 工学部, 准教授 (10332868)
Co-Investigator(Renkei-kenkyūsha) HAYASHIDA Osamu  福岡大学, 理学部, 教授 (20231532)
Project Period (FY) 2008 – 2011
KeywordsDNAコンジュゲート / 協同性 / 化学センサー / バイオセンサー / 光化学 / 錯生成 / ハイブリダイゼーション / 機能性核酸
Research Abstract

The research aims at the development of the DNA and PNA-based functional modules and their integrated flexible system for chemical or physical signal amplification and transduction. DNA conjugates carryingβ-cyclodextrin, terpyridine, and anthracene were prepared as functional modules for fluorescent SNP analysis, metallo-regulation of DNA functions, and reversible photo-regulation of DNA-based nanostructures, respectively.

  • Research Products

    (42 results)

All 2012 2011 2010 2009 2008 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (20 results) Book (4 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 1 results)

  • [Journal Article] Photochemically Relevant DNA-based Molecular Systems Enabling Chemical and Signal Transductions and Their Analytical Applications2012

    • Author(s)
      T. Ihara, Y. Kitamura
    • Journal Title

      J. Photochem. Photobiol. C

      Volume: (印刷中)

    • Peer Reviewed
  • [Journal Article] スプリット型プローブの協同的錯体形成を利用するDNAの認識及び検出2012

    • Author(s)
      井原敏博, 北村裕介
    • Journal Title

      分析化学

      Volume: 61巻 Pages: 193-206

    • Peer Reviewed
  • [Journal Article] DNA Analysis Based on the Local Structural Disruption on the Duplexes Carrying a Luminous Lanthanide Complex2011

    • Author(s)
      T. Ihara, Y. Kitamura, Y. Tsujimura, A. Jyo
    • Journal Title

      Anal. Sci

      Volume: 27巻 Pages: 585-590

    • Peer Reviewed
  • [Journal Article] Electrochemical Signal Modulation in Homogeneous Solutions Using the Formation of an Inclusion Complex between Ferrocene andβ-cyclodextrin on DNA Scaffold2011

    • Author(s)
      T. Ihara, T. Wasano, R. Nakatake, P. Arslan, A. Futamura, A. Jyo
    • Journal Title

      Chem. Commun

      Volume: 47巻 Pages: 12388-12390

    • Peer Reviewed
  • [Journal Article] Reversible Circularization of an Anthracene-modified DNA Conjugate through Bimolecular Triplex Formation and Its Analytical Application2010

    • Author(s)
      P. Arslan, A. Jyo, T. Ihara
    • Journal Title

      Org. Biomol. Chem.

      Volume: 8巻 Pages: 4843-4848

    • Peer Reviewed
  • [Journal Article] Cooperative DNA Probing Using aβ-Cyclodextrin. DNA Conjugates and a Nucleobase-specific Fluorescent Ligand2009

    • Author(s)
      T. Ihara, A. Uemura, A. Futamura, M. Shimizu, N. Baba, S. Nishizawa, N. Teramae, A. Jyo
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 131巻 Pages: 1386-1387

    • Peer Reviewed
  • [Journal Article] Anthracene-DNA Conjugates as Building Blocks of Designed DNA Structures Constructed by Photochemical Reactions2009

    • Author(s)
      M. Mukae, T. Ihara, M. Tabara, A. Jyo
    • Journal Title

      Org. Biomol. Chem.

      Volume: 7巻 Pages: 1349-1354

    • Peer Reviewed
  • [Journal Article] Silver Ion Unusually Stabilizes the Structure of a Parallel-Motif DNA Triplex2009

    • Author(s)
      T. Ihara, T. Ishii, N. Araki, A. W. Wilson, A. Jyo
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 131巻 Pages: 3826-3827

    • Peer Reviewed
  • [Journal Article] Photodimerization of Anthracenes Using a DNA Template and Its Analytical Applications2009

    • Author(s)
      M. Mukae, T. Ihara, M. Tabara, P. Arslan, A. Jyo
    • Journal Title

      Supramol. Chem.

      Volume: 21巻 Pages: 292-295

    • Peer Reviewed
  • [Journal Article] DNA Conjugates Bearing a Ferrocenyl Group in Backbone and Their Electrochemical Behavior2009

    • Author(s)
      T. Ihara, D. Sasahara, M. Shimizu, A. Jyo
    • Journal Title

      Supramol. Chem.

      Volume: 21巻 Pages: 207-217

    • Peer Reviewed
  • [Journal Article] Efficient Guest Inclusion byβ-Cyclodextrin Attached to the Ends of DNA Oligomers upon Hybridization to Various DNA Conjugates2009

    • Author(s)
      A. Kuzuya, T. Ohnishi, T. Wasano, S. Nagaoka, J. Sumaoka, T. Ihara, A. Jyo, M. Komiyama
    • Journal Title

      Bioconjugate Chem.

      Volume: 20巻 Pages: 1643-1649

    • Peer Reviewed
  • [Journal Article] The Effect of Local Structural Disruption on the Yield of Photochemical Ligation between Anthracene-Oligonucleotide Conjugates2008

    • Author(s)
      P. Arslan, T. Ihara, M. Motoko, A. Jyo
    • Journal Title

      Anal. Sci.

      Volume: 24巻 Pages: 173-176

    • Peer Reviewed
  • [Journal Article] Template-Directed Formation of Luminescent Lanthanide Complexes : Versatile Tools for Colorimetric Identification of Single Nucleotide Polymorphism2008

    • Author(s)
      Y. Kitamura, T. Ihara, Y. Tsujimura, Y. Osawa, D. Sasahara, M. Yamamoto, K. Okada, M. Tazaki, A. Jyo
    • Journal Title

      J. Inorg. Biochem.

      Volume: 102巻 Pages: 1921-1931

    • Peer Reviewed
  • [Journal Article] Metallo-regulation of Triple Helix Formation by Control of the Loop Conformation2008

    • Author(s)
      T. Ihara, Y. Sato, H. Shimada, A. Jyo
    • Journal Title

      Nucleos. Nucleot. Nucl.

      Volume: 27巻 Pages: 1084-1096

    • Peer Reviewed
  • [Presentation] 核酸上でのデザインされた特異反応およびその分析化学的応用2012

    • Author(s)
      井原敏博
    • Organizer
      第20回アンチセンスシンポジウム
    • Place of Presentation
      甲南大学,神戸市
    • Year and Date
      2012-12-02
  • [Presentation] Electrochemical Study of DNA Hybridization in Homogeneous Solutions2011

    • Author(s)
      T. Ihara, T. Wasano, R. Nakatake, A. Futamura, A. Jyo
    • Organizer
      The 38th International Symposium on Nucleic Acid Chemistry, ISNAC2011
    • Place of Presentation
      北海道大学, Sapporo
    • Year and Date
      2011-11-09
  • [Presentation] Rational regulation of nucleic acid structures by specific formation of metal complexes2011

    • Author(s)
      T. Ihara
    • Organizer
      The 14th Asian Chemical Congress
    • Place of Presentation
      Bangkok,タイ王国
    • Year and Date
      2011-09-07
  • [Presentation] 機能性核酸複合体のプログラミングと分析化学的応用2011

    • Author(s)
      井原敏博
    • Organizer
      中央大学理工学研究科学術講演会
    • Place of Presentation
      中央大学,東京
    • Year and Date
      2011-07-15
  • [Presentation] DNA probing by cooperative luminous complex formation on the target2010

    • Author(s)
      T. Ihara, Y. Kitamura, A. Uemura, N. Baba, S. Nishizawa, N. Teramae, A. Jyo
    • Organizer
      PacifiChem 2010
    • Place of Presentation
      Honolulu,アメリカ合衆国
    • Year and Date
      2010-12-16
  • [Presentation] Cooperative DNA Probing Using-Cyclodextrin-modified DNA and Nucleobase-specific Fluorescent Ligand2010

    • Author(s)
      A. Futamura, A Uemura, A. Jyo, N. Baba, S. Nishizawa, N. Teramae, T. Ihara
    • Organizer
      PacifiChem 2010
    • Place of Presentation
      Honolulu,アメリカ合衆国
    • Year and Date
      2010-12-16
  • [Presentation] Synthesis of DNA Conjugate for Photo-triggered New Chemical Ligation2010

    • Author(s)
      T. Hirayama, A. Jyo, T. Ihara
    • Organizer
      PacifiChem 2010
    • Place of Presentation
      Honolulu,アメリカ合衆国
    • Year and Date
      2010-12-16
  • [Presentation] Rational Construction of Luminous Structures on DNA Scaffold for Analytical Applications2010

    • Author(s)
      T. Ihara, A. Futamura, A. Uemura, Y. Kitamura, A. Jyo, Y. Sato, S. Nishizawa, N. Teramae
    • Organizer
      The 19th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids(IRTXIX)
    • Place of Presentation
      Lyon,フランス
    • Year and Date
      2010-08-29
  • [Presentation] 機能分子を協調させて核酸を検出する2010

    • Author(s)
      井原敏博
    • Organizer
      奈良先端科学技術大学院大学物質創成化学特講
    • Place of Presentation
      奈良先端大学院大学,奈良市
    • Year and Date
      2010-02-10
  • [Presentation] Interaction of silver ion with CG. C^+base triplets in DNA triplex2009

    • Author(s)
      T. Ihara, T. Ishii, A. Jyo
    • Organizer
      The 6th International Symposium on Nucleic Acids Chemistry 2009(6ISNAC2009)
    • Place of Presentation
      高山文化会館, Takayama
    • Year and Date
      2009-09-28
  • [Presentation] DNA Probing Using Luminous Complexes Assembled on the Targets2009

    • Author(s)
      T. Ihara
    • Organizer
      2nd Switzerland-Japan Biomolecular Chemistry Symposium(SJBCS 2009)
    • Place of Presentation
      東京大学, Tokyo
    • Year and Date
      2009-09-11
  • [Presentation] 核酸上での分子間相互作用を利用したシグナル変換2008

    • Author(s)
      井原敏博
    • Organizer
      第11回生命化学研究会-生命化学をシステムで捉えたら-
    • Place of Presentation
      ホテル水上館,群馬県利根郡みなかみ町
    • Year and Date
      2008-11-28
  • [Presentation] スプリットプローブの形成する希土類錯体の発光を利用した遺伝子検出2008

    • Author(s)
      井原敏博
    • Organizer
      第58回錯体化学討論会(希土類錯体:未踏配位化学の探求と機能創成)
    • Place of Presentation
      金沢大学,金沢市
    • Year and Date
      2008-09-20
  • [Presentation] Efficient Circular DNA Formation by Photochemical Ligation on the Bimolecular Triplex Consisting of Homopyrimidine Template and Anthracene-modified Oligonucleotide Conjugate2008

    • Author(s)
      P. Arslan, T. Ihara, A Jyo
    • Organizer
      Joint Symposium of 18^<th> International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and 35^<th> International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      京都国立国際会館, Kyoto
    • Year and Date
      2008-09-10
  • [Presentation] 核酸上での分子間相互作用を利用したシグナル変換2008

    • Author(s)
      井原敏博
    • Organizer
      日本分析化学会第57年会
    • Place of Presentation
      福岡大学,福岡市
    • Year and Date
      2008-09-10
  • [Presentation] Photodimerization of Anthracene Using DNA Template and Its Analytical Application2008

    • Author(s)
      M. Mukae, T. Ihara, M. Tabara, P. Arslan, A. Jyo
    • Organizer
      International Symposium on Macrocyclic and Supramolecular Chemistry 2008(ISMSC2008)
    • Place of Presentation
      Las Vegas,アメリカ合衆国
    • Year and Date
      2008-07-17
  • [Presentation] Electrochemical Gene Analysis Based on Cooperative Interaction of DNA Conjugates2008

    • Author(s)
      T. Ihara, T. Wasano, A. Uemura, D. Sasahara, M. Shimizu, A. Jyo
    • Organizer
      International Symposium on Macrocyclic and Supramolecular Chemistry 2008(ISMSC2008)
    • Place of Presentation
      Las Vegas,アメリカ合衆国
    • Year and Date
      2008-07-17
  • [Presentation] 機能化DNAの協同性を利用した核酸の認識及び検出2008

    • Author(s)
      井原敏博
    • Organizer
      第45回化学関連支部合同九州大会
    • Place of Presentation
      北九州国際会議場,北九州市
    • Year and Date
      2008-07-05
  • [Presentation] 核酸を足場とした超分子設計-計測系への応用-2008

    • Author(s)
      井原敏博
    • Organizer
      第24回緑陰セミナー札幌
    • Place of Presentation
      国営滝野すずらん丘陵公園青少年自然の家,札幌市
    • Year and Date
      2008-06-29
  • [Presentation] プローブを協調させてDNAを認識・分析する2008

    • Author(s)
      井原敏博
    • Organizer
      大阪大学大学院薬学研究科特別講演会
    • Place of Presentation
      大阪大学,吹田市
    • Year and Date
      2008-05-16
  • [Book] 核酸とカチオンの特異な相互作用、CSJカレントレビュー核酸化学のニュートレンド2011

    • Author(s)
      井原敏博, 他
    • Total Pages
      173-180
    • Publisher
      化学同人
  • [Book] 基礎教育シリーズ分析化学(基礎編)2011

    • Author(s)
      井原敏博, 他
    • Publisher
      東京教学社
  • [Book] 基礎教育シリーズ分析化学(機器分析編)2011

    • Author(s)
      井原敏博, 他
    • Publisher
      東京教学社
  • [Book] 基礎教育シリーズ新版分析化学実験2008

    • Author(s)
      井原敏博, 他
    • Publisher
      東京教学社
  • [Remarks]

    • URL

      http://133.95.131.186/~toshi/

  • [Patent(Industrial Property Rights)] Method of Electrochemically Detecting Nucleic Acid with the Use of DNA Conjugate2009

    • Inventor(s)
      井原敏博
    • Industrial Property Rights Holder
      熊本大学
    • Industrial Property Number
      特許、PCT/JP2009/054542
    • Filing Date
      2009-03-10
    • Overseas
  • [Patent(Industrial Property Rights)] 金属イオンにより核酸の多重構造を安定化させる方法2009

    • Inventor(s)
      井原敏博
    • Industrial Property Rights Holder
      熊本大学
    • Industrial Property Number
      特許、2009-032783
    • Filing Date
      2009-02-16
  • [Patent(Industrial Property Rights)] DNAコンジュゲートを利用した核酸の電気化学的検出法2008

    • Inventor(s)
      井原敏博
    • Industrial Property Rights Holder
      熊本大学
    • Industrial Property Number
      特許、2008-168127
    • Filing Date
      2008-06-27

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Published: 2013-07-31  

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