2011 Fiscal Year Final Research Report
Functional Organization of super-molecular complex with the function of photo-electric conversion
| Project/Area Number |
20350053
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Polymer chemistry
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| Research Institution | Osaka City University (2010-2011)
Nagoya Institute of Technology (2008-2009) |
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Principal Investigator |
NANGO Mamoru 大阪市立大学, 複合先端研究機構, 特任教授 (90109893)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Keiji 名古屋工業大学, 工学研究科, 教授 (90158155)
DEWA Takehisa 名古屋工業大学, 工学研究科, 准教授 (70335082)
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| Project Period (FY) |
2008 – 2011
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| Keywords | 生体関連高分子 / 人工光合成 / 光合成 / アンテナ色素複合体 / 自己組織化 / 光電 |
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| Research Abstract |
The purpose of this proposal is to use photosynthetic antenna pigment complexes, LH2 and LH1-RC of purple photosynthetic bacteria in order to control the direction and orientation of the complex on electrodes with pattern for developing nano-biodevices. The pigment-protein complexes of the modified LH1-RC or LH2 complex was laid down onto functionalized electrodes, such as ITO or Au electrode. Upon illumination photocurrents were successfully measured. Excitation spectra confirmed that these photocurrents were produced by light absorbed by the pigment-protein complexes. Further, LH1 synthetic model polypeptides, analogous to the native LH polypeptide were assembled on Au or ITO electrode. Pigments such as native and chlorophyll derivatives were selected and assembled on the specific site of the LH1 synthetic model polypeptides to control the organization of the pigments on electrodes. The structural effects of the pigments and the synthetic polypeptides in lipid bilayers on the production of the efficient photocurrent were examined as well as using electro-conductive AFM study. These methods were useful for the self-assembly of these complexes in order to study the energy transfer and electron transfer reactions between individual pigments and hydrophobic or polar amino acids in the complexes on the electrode.
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[Journal Article] Immobilization and Photocurrent Activity of a Light-Harvesting Antenna Complex II, LHCII Isolated from a Plant on Electrodes2012
Author(s)
M. Nagata, M. Amano, T. Joke, K. Fuji, A. Okuda, M. Kondo, S. Ishigure, T. Dewa, K. Iida, F. Secundo, Y. Amao, H. Hashimoto, M. Nango
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Journal Title
ACS Macro Lett.
Volume: 1
Pages: 296-299
DOI
Peer Reviewed
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[Journal Article] Two-Dimensional Molecular Assembly of Bacteriochlorophyll a Derivatives Using Synthetic Poly(Ethylene Glycol)-Linked Light-Harvesting Model Polypeptides on a Gold Electrode Modified with Supported Lipid Bilayers2012
Author(s)
T. Ochiai, M. Nagata, K. Shimoyama, T. Kato, T. Asaoka, M. Kondo, T. Dewa, K. Yamashita, A. Kashiwada, S. Futaki, H. Hashimoto, M. Nango
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Journal Title
ACS Macro Lett.
Volume: 1
Pages: 28-22
DOI
Peer Reviewed
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[Journal Article] Photocurrent and Electronic Activities of Oriented-His-tagged Photosynthetic Light-Harvesting/Reaction Centre Core Complexes Assembled onto a Gold Electrode2012
Author(s)
M. Kondo, K. Iida, T. Dewa, H. Tanaka, T. Ogawa, S. Nagashima, K. V. P. Nagashima, K. Shimada, H. Hashimoto, A. T. Gardiner, R. J. Cogdell, M. Nango
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Journal Title
Biomacromolecules
Volume: 13
Pages: 432-438
DOI
Peer Reviewed
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[Journal Article] Construction and Structural Analysis of Tethered Lipid Bilayer Containing Photosynthetic Antenna Proteins for Functional Analysis2011
Author(s)
A. Sumino, T. Dewa, T. Takeuchi, R. Sugiura, N. Sasaki, N. Misawa, R. Tero, T. Urisu, A. T. Gardiner, R. J. Cogdell, H. Hashimoto, M. Nango
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Journal Title
Biomacromolecules
Volume: 12
Pages: 2850-2858
DOI
Peer Reviewed
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