2010 Fiscal Year Final Research Report
Detection of miRNAs using artificial oligonucleotide probes capable of chain length discrimination
| Project/Area Number |
20350074
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
SEIO Kohji Tokyo Institute of Technology, 大学院・生命理工学研究科, 准教授 (20313356)
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| Project Period (FY) |
2008 – 2010
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| Keywords | microRNA / 鎖長識別能 / 短鎖RNA選択的結合 / 人工核酸 / 塩基識別能 / 蛍光核酸 |
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| Research Abstract |
Artificial oligonucleoptide probes were developed for the detection of mature miRNA in living cells. The new nucleic acids derivatives such as the oligonucleotide probes having short RNA selectivity, the nucleic acid analogs capable of precise base recognition and the fluorescent nucleic acids applicable for the monitoring of the hybridization events were developed. These artificial nucleic acids are useful for the miRNA detection.
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[Journal Article]2011
Author(s)
Tsunoda, H., Kudo, T., Masaki, Y., Ohkubo, A., Seio K., Sekine M.
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Journal Title
Nucleic Acids Research 39
Pages: 2995-3004
Peer Reviewed
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[Journal Article] Synthesis of terminally modified oligonucleotides and their hybridization dependence on the size of the target RNAs.2009
Author(s)
Seio, K., Takaku, Y., Miyazaki, K., Kurohagi, S., Masaki, Y., Ohkubo, A., Sekine, M.
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Journal Title
Organic & Biomolecular Chemistry 7
Pages: 2440-2451
Peer Reviewed
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[Journal Article] Synthesis and hybridization of 20-O-methyl-RNAs incorporating 2'-O-carbamoyluridine and unique participation of the carbamoyl group in U.G base pair.2009
Author(s)
Seio, K., Tawarada, R., Sasami, T., Serizawa, M., Ise, M., Ohkubo, A., Sekine, M.
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Journal Title
Bioorganic & Medicinal Chemistry 17
Pages: 7275-7280
Peer Reviewed
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[Journal Article] Hybridization-dependent fluorescence of oligodeoxynucleotides incorporating new pyrene-modified adenosine residues.2008
Author(s)
Seio, K., Mizuta, M., Tasaki, K., Tamaki, K., Ohkubo, A., Sekine, M.
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Journal Title
Bioorganic & Medicinal Chemistry 16
Pages: 8287-8293
Peer Reviewed
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