2010 Fiscal Year Final Research Report
Designing novel functions of proteins by using an artificial genetic code dictionary
| Project/Area Number |
20350075
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Gifu University |
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Principal Investigator |
YOKOGAWA Takashi Gifu University, 工学部, 准教授 (90242304)
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| Co-Investigator(Renkei-kenkyūsha) |
OHNO Satoshi 岐阜大学, 工学部, 助教 (10345796)
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| Project Period (FY) |
2008 – 2010
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| Keywords | タンパク質合成系 / PURE システム / tRNA / 固相化プローブ法 / 遺伝暗号表 / 非天然アミノ酸 / Click chemistry |
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| Research Abstract |
Restriction of the number of endogenous tRNAs in protein synthesis system causes "empty codons" in the genetic code dictionary. If we can create a tRNA that reads "an empty codon" and accepts an unnatural amino acid, it means that we develop an artificial genetic code dictionary. Here, I purified 21 tRNAs from Escherichia coli, and made a minimal tRNA set by remixing an individual tRNA. Actually, yellow fluorescent protein could be synthesized in PURE system with a minimal tRNA set and a chemically synthesized gene having minimal codons. It indicates that proteins having several unnatural amino acids can be effectively synthesized in the future.
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