2010 Fiscal Year Final Research Report
Precise synthesis of glycoproteins having human oligosaccharides by use of chemical and expression methodologies.
| Project/Area Number |
20350081
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Chemistry related to living body
|
|---|
| Research Institution | Yokohama City University |
|---|
Principal Investigator |
KAJIHARA Yasuhiro Yokohama City University, 大学院・理学研究科, 教授 (50275020)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | 糖タンパク質 / 糖鎖 / 糖ペプチド |
|---|
| Research Abstract |
Erythropoietin analogue and co-stimulate glycoprotein were synthesized. In addition to these results, glycosylated polypeptide chain of IgG-Fc region was also synthesized. In order to synthesize glycoproteins, glycopeptides-thioesters were prepared by chemical method and polypeptide chain was prepared from E. Coli expression method. Coupling both peptides to prepare whole glycosylated polypeptide and subsequent folding process afforded the folded glycoproteins.
|
