Control of Orientation and Shape of Adult Stem Cell on Base Surface by Negative-Ion Pattern Implantation and Induction of The Differentiation

2010 Fiscal Year Final Research Report

• Project/Area Number: 20360018
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thin film/Surface and interfacial physical properties
• Research Institution: Kyoto University
• Principal Investigator: TSUJI Hiroshi Kyoto University, 大学院・工学研究科, 助教 (20127103)
• Co-Investigator(Kenkyū-buntansha): ISHIKAWA Junzo 京都大学, 大学院・工学研究科, 教授 (80026278) ; TAKAOKA Gikan 京都大学, 大学院・工学研究科, 教授 (90135525) ; SATO Hiroko 京都大学, 大学院・工学研究科, 研究員 (00093245) ; SOMMNI Piyanuch 京都大学, 大学院・工学研究科, 研究員 (40512968)
• Project Period (FY): 2008 – 2010
• Keywords: 負イオン注入 / 表面改質 / 細胞親和性 / 間葉系幹細胞 / 神経回路網 / 細胞チップ

Research Abstract

Mesenchymal stem cells (MSC) derived from bone-mallow of rat showed their self-aligned adhesion on polymer surfaces along the modified line-region in which carbon negative-ions were implanted through line-pattern mask for improving hydrophilicity. Besides, the adhered cells were differentiated into nerve cells and osteoblast cells with holding their adhesion pattern. By narrowing the width of the line pattern, the orientation of MSC cell body and nuclei were controlled to along with the long direction of the line pattern of the ion implantation. Cell sheet and string were formed by using C and O negative ion implantation into poly-lactic acid.

Research Products

• [Journal Article] Surface Modification of Silica Glass by CHF3 Plasma Treatment and Carbon Negative-Ion Implantation for Cell Pattern Adhesion (2011)
  • Author(s): H.Tsuji, P.Sommani, Y.Hayashi, H.Kojima, H.Sato, Y.Gotoh, G.H.Takaoka, J.Ishikawa
  • Journal Title: Surface and Coatings Technology to be published
  • Peer Reviewed
• [Journal Article] Line-Width of Ion Beam-Modified Polystyrene by Negative Carbon Ions for Fine Adhesion Pattern of Mesenchymal Stem Cells (2011)
  • Author(s): P.Sommani, H.Tsuji, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Journal Title: Surface and Coatings Technology to be published
  • Peer Reviewed
• [Journal Article] Irradiation effect of carbon negative-ion implantation on polytetrafluoroethylene for controlling cell- adhesion property (2010)
  • Author(s): P.Sommani, H.Tsuji, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Journal Title: Nuclear Instruments and Methods in Physics Research B Vol.B268 Pages: 3231-3234
  • Peer Reviewed
• [Journal Article] Surface Modification of Silicone Rubber for Adhesion Patterning of Mesenchymal Stem Cells by Water Cluster Ion Beam (2010)
  • Author(s): P.Sommani, G.Ichihashi, H.Ryuto, H.Tsuji, Y.Gotoh, G.H.Takaoka
  • Journal Title: Ion Implantation 2010(Eds : J.Matsuo, M.Kase, T.Aoki, T.Seki, American Institute of Physics, 2010) Vol.IIT2010 Pages: 302-305
  • Peer Reviewed
• [Journal Article] Surface Modification by Negative-Ion Implantation (2009)
  • Author(s): J.Ishikawa, H.Tsuji, Y.Gotoh
  • Journal Title: Surface and Coatings Technology Vol.203 Pages: 2351-2356
  • Peer Reviewed
• [Journal Article] Negative Ion Implantation for Pattering Mesenchymal Stem Cell Adhesion on Silicone Rubber and Differentiation into Nerve Cells with Preserving Their Adhesion Pattern (2009)
  • Author(s): H.Tsuji, P.Sommani, M.Hattori, T.Yamada, H.Sato, Y.Gotoh, J.Ishikawa
  • Journal Title: Surface and Coatings Technology Vol.203 Pages: 2562-2565
  • Peer Reviewed
• [Journal Article] Adhesion Patterning of Mesenchymal Stem Cells on Polystyrene Surface by Carbon Negative-Ion Implantation and Neuron Differentiation on the Position (2008)
  • Author(s): H.Tsuji, P.Sommani, M.Hattori, T.Yamada, H.Sato, Y.Gotoh, J.Ishikawa
  • Journal Title: Nuclear Instruments and Methods in Physics Research Vol.B266 Pages: 3067-3070
  • Peer Reviewed
• [Presentation] Ion Patterned Adhesion for Neuron Network Synthesis (2011)
  • Author(s): H.Tsuji
  • Organizer: European Material Research Society Spring Meeting E-MRS 2011, B4-1(Invited)
  • Place of Presentation: Nice, France
  • Year and Date: 2011-05-10
• [Presentation] Osteoblast Patterning on Silicone Rubber by using Mesenchymal Stem Cells and Carbon Negative-Ion (2010)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: 21st International Academic Symposium of Materials Research Society of Japan, MRS-J2010, S-12-G
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2010-12-22
• [Presentation] Pattern modification of polymer surface by negative ion beam for neuron network formation (2010)
  • Author(s): H.Tsuji, P.Sommani, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: International Negative Ion Beam Symposium, NIBS2010, O-42
  • Place of Presentation: Takayama, Japan.
  • Year and Date: 2010-11-18
• [Presentation] Ion Beam Modification of Polymers for Self-Aligned Adhesion of Mesenchymal Stem Cells and Control of Nuclei Orientation (2010)
  • Author(s): H.Tsuji, P.Sommani, T.Yamada, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Organizer: 10th European Conference on Accelerators in Applied Research and Technology, ECAART 10, PI-14
  • Place of Presentation: Grifada, Greek.
  • Year and Date: 2010-09-13
• [Presentation] Negative-ion beam degradation of proteins for adhesion patterning of brain neuronal cells (2010)
  • Author(s): H.Tsuji, P.Sommani, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: 17th International Conference on Ion Beam Modification of Materials, IBMM2010, #336
  • Place of Presentation: Montreal, Canada.
  • Year and Date: 2010-08-24
• [Presentation] Negativeion beam modified polymeric surfaces for controls of individual cell arrangement and cell-nucleus orientation (2010)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, T.Yamada, H.Kojima, Y.Gotoh, J.Ishikawa
  • Organizer: 17th International Conference on Ion Beam Modification of Materials, IBMM2010, #455
  • Place of Presentation: Montreal, Canada.
  • Year and Date: 2010-08-24
• [Presentation] Surface Modification of Silicone Rubber for Adhesion Patterning of Mesenchymal Stem Cells by Water Cluster Ion Beam (2010)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: 18th International Conference on Ion Implantation Technology, IIT2010, P2-58
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-07-07
• [Presentation] Carbon Negative-Ion Implantation for Positive- Pattern of Avidin on Polystyrene (2009)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: 20th International Academic Symposium of MRS-J2009, R-17-G
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2009-12-09
• [Presentation] Cell Adhesion Pattering on Polystyrene by Using Avidin Coating and Carbon Negative-Ion Implantation (2009)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: 20th International Academic Symposium of MRS-J2009, R-P05-G
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2009-12-08
• [Presentation] Degradation of Proteins for Neuronal-Cell-Adhesion Pattering by Carbon Negative-Ion Implantation (2009)
  • Author(s): H.Tsuji, P.Sommani, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: 20th International Academic Symposium of MRS-J2009, R-P06
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2009-12-08
• [Presentation] Surface Modification of Silica Glass by CHF3 Plasma Treatment and Carbon Negative-Ion Implantation for Cell Pattern Adhesion (2009)
  • Author(s): H.Tsuji, P.Sommani, Y.Hayashi, H.Kojima, H.Sato, Y.Gotoh, G.H.Takaoka, J.Ishikawa
  • Organizer: 16th International Conference on Surface Modification of Materials by Ion Beam, SMMIB 2009, PC-06
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2009-09-16
• [Presentation] Line-Width of Ion Beam-Modified Polystyrene by Negative Carbon Ions for Fine Adhesion Pattern of Mesenchymal Stem Cells (2009)
  • Author(s): P.Sommani, H.Tsuji, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Organizer: 16th International Conference on Surface Modification of Materials by Ion Beam, SMMIB2009, PC-13
  • Place of Presentation: Tokyo, Japan.
  • Year and Date: 2009-09-16
• [Presentation] Carbon Negative-Ion Implantation on Silicone Rubber with Narrower Line Width for Pattern Adhesion of Mesenchymal Stem Cells and Control of Nuclei Orientation (2009)
  • Author(s): H.Tsuji, P.Sommani, T.Yamada, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Organizer: 15th International Conference on Radiation Effects in Insulators, REI-15, C20
  • Place of Presentation: Padova, Italy
  • Year and Date: 2009-09-03
• [Presentation] Radiation Effect of Carbon Negative-Ion Implantation on Polytetrafluoroethylene for Controlling Cell-Adhesion Property (2009)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, T.Yamada, H.Kojima, Y.Gotoh, J.Ishikawa
  • Organizer: 15th International Conference on Radiation Effects in Insulators, REI-15, C22
  • Place of Presentation: Padova, Italy.
  • Year and Date: 2009-09-03
• [Presentation] Mesenchymal Stem Cell Patterning and Its Differentiation into Neuron on Silicone Rubber Modified by Carbon Negative-IoImplantation (2009)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, T.Yamada, H.Yamada, Y.Gotoh, J.Ishikawa
  • Organizer: 2nd Asian Biomaterials Congress 2009 in conjunction with IUMRS-ICA2009, ABMC-1-07P
  • Place of Presentation: Singapore
  • Year and Date: 2009-07-26
• [Presentation] Adhesion Patterning of Mesenchymal Stem Cells on Silicone Rubber by Carbon Negative-Ion Implantation for Artificial Nerve-Cell Network Formation(invited talk) (2009)
  • Author(s): H.Tsuji
  • Organizer: Huntsville Ion Beam Institute, HIBI2009
  • Place of Presentation: Huntsville, AL USA.
  • Year and Date: 2009-07-14
• [Presentation] Minimum Line-Width on Silicone Rubber Modified by Carbon Negative-Ion Implantation for Self-Aligned Adhesion of Mesenchymal Stem Cells and Orientation Control of Nucleus (2009)
  • Author(s): H.Tsuji, P.Sommani, H.Kojima, Y.Hayashi, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: 2nd Asian Biomaterials Congress 2009 in conjunction with IUMRS-ICA2009, ABMC-1-06P
  • Place of Presentation: Singapore.
  • Year and Date: 2009-06-26
• [Presentation] Nuclei Orientation of Mesenchymal Stem Cells on Narrow Regions of Silicone Rubber Modified by Carbon Negative-Ion Implantation (2008)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, H.Kojima, Y.Gotoh, J.Ishikawa
  • Organizer: IUMRS International Conference in Asia 2008, IUMRS-ICA2008, MO-3
  • Place of Presentation: Nagoya, Japan.
  • Year and Date: 2008-12-10
• [Presentation] Comparison of Implantation Conditions of Carbon Negative Ions for Pattern Adhesion of Mesenchymal Stem Cells on Polymeric Surfaces (2008)
  • Author(s): H Tsuji, P Sommani, T Yamada, H Kojima, H Sato, Y Gotoh, o Ishikawa
  • Organizer: IUMRS International Conference in Asia 2008, IUMRS-ICA2008, MP-3
  • Place of Presentation: Nagoya, Japan.
  • Year and Date: 2008-12-10
• [Presentation] Effect of Decreasing Line-Width in Carbon Negative-Ion Pattern-Implantation into Polystyrene Surface upon Adhesion of Mesenchymal Stem Cells (2008)
  • Author(s): H.Tsuji, P.Sommani, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Organizer: 4th Vacuum and Surface Sciences Conference of Asia and Australia, VASSCAA-4, 28P100
  • Place of Presentation: Matsue, Japan.
  • Year and Date: 2008-10-28
• [Presentation] C-implantation for Avidin Patterning on Polystyrene (2008)
  • Author(s): P.Sommani, H.Tsuji, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Organizer: The 4th Vacuum and Surface Sciences Conference of Asia and Australia, VASSCAA-4, 28P099
  • Place of Presentation: Matsue, Japan.
  • Year and Date: 2008-10-28
• [Presentation] Modified Minimum Line-Width in Carbon Negative-Ion Implantation for Self-Aligned Adhesion of Mesenchymal Stem Cells on Silicone Rubber and Orientation Control of Nucleus (2008)
  • Author(s): H.Tsuji, P.Sommani, T.Yamada, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Organizer: 16th International Conference on Ion Beam Modification of Materials, IBMM08, PB14-03
  • Place of Presentation: Dresden, Germany.
  • Year and Date: 2008-09-03
• [Presentation] Negative-Ion Beam Modified Silicone Rubber for Patterning Adhesions of Mesenchymal Stem Cell and Its Differentiated Neurons (2008)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, T.Yamada, H.Kojima, Y.Gotoh, J.Ishikawa
  • Organizer: 16th International Conference on Ion Beam Modification of Materials, IBMM08, PB14-04
  • Place of Presentation: Dresden, Germany.
  • Year and Date: 2008-09-03
• [Presentation] Principal Concepts in Develop- ment of Ion Sources in Kyoto University (invited) (2008)
  • Author(s): J.Ishikawa
  • Organizer: 9th International Conference on Applications of Nuclear Techniques, CRETE08
  • Place of Presentation: Crete, Greek.
  • Year and Date: 2008-06-12
• [Presentation] Neuron Arrangement on Silicone Rubber by Using Differentiation from Mesenchymal Stem Cells and Carbon Negative-Ion Implantation (2008)
  • Author(s): H.Tsuji, P.Sommani, M.Hattori, T.Yamada, H.Sato, Y.Gotoh, J.Ishikawa
  • Organizer: 9th International Conference on Applications of Nuclear Techniques, CRETE08
  • Place of Presentation: Crete, Greek.
  • Year and Date: 2008-06-12