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2010 Fiscal Year Final Research Report

Nonlinear-optical microscopy for visualizing chiral structures in biological molecules

Research Project

  • PDF
Project/Area Number 20360033
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied optics/Quantum optical engineering
Research InstitutionOsaka University

Principal Investigator

ITO Kazuyoshi  Osaka University, 工学研究科, 教授 (80113520)

Co-Investigator(Kenkyū-buntansha) OZEKI Yasuyuki  大阪大学, 工学研究科, 助教 (60437374)
KAJIYAMA Shinichiro  近畿大学, 生物理工学部, 准教授 (20243496)
Project Period (FY) 2008 – 2010
Keywords光計測 / 光学顕微鏡 / 超短光パルス / 非線形光学効果
Research Abstract

We have developed new optical microscopic imaging techniques which exploit the electronic and vibrational resonances in nonlinear-optical processes. Specifically, we demonstrated (a) the visualization of two-photon absorption resonances in red blood cells and plant petal cells based on stimulated parametric emission microscopy, and (b) background-free imaging of molecular vibrations based on stimulated Raman scattering microscopy.

  • Research Products

    (34 results)

All 2011 2010 2009 2008 Other

All Journal Article (9 results) Presentation (21 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Stimulated Ramanscattering microscope with shot noiselimited sensitivity usingsubharmonically synchronized laserpulses2010

    • Author(s)
      Y. Ozeki, Y. Kitagawa, K. Sumimura, N.Nishizawa, W. Umemura, S. Kajiyama, K.Fukui, K. Itoh
    • Journal Title

      Opt.Express vol.18, no.13

      Pages: 13708-13719

  • [Journal Article] StimulatedRaman scattering microscopy for livecell imaging with high contrast andhigh sensitivity2010

    • Author(s)
      Y. Ozeki, K. Itoh
    • Journal Title

      Laser Phys. vol.20, no. 5

      Pages: 1114-1118

  • [Journal Article] Dual-band stimulated parametricemission microscopy2010

    • Author(s)
      M. Yamagiwa, G. Omura, Y. Ozeki, M.Ishii, H. M. Dang, S. Kajiyama, T.Suzuki, K. Fukui, K. Itoh
    • Journal Title

      Jpn.J.Appl.Phys. vol.49

      Pages: 016603

  • [Journal Article] Anlysis and compensation forartifacts in three-dimensionalrefractive index profiling byfour-wave mixing microscopy2010

    • Author(s)
      T. Kawasumi, Y. Ozeki, K. Itoh
    • Journal Title

      Jpn.J.Appl.Phys. vol.49

      Pages: 082701

  • [Journal Article] Depth-resolved observation ofphotoelastic effect by four-wavemixing microscopy2009

    • Author(s)
      Y. Ozeki, T. Kawasumi, K. Itoh
    • Journal Title

      Opt.Rev. vol.16, no. 2

      Pages: 167-169

  • [Journal Article] Three-dimensionalunstained live-cell imaging usingstimulated parametric emissionmicroscopy2009

    • Author(s)
      H. M. Dang, T. Kawasumi, G. Omura, T.Umano, S. Kajiyama, Y. Ozeki, K. Itoh, K. Fukui
    • Journal Title

      Jpn.J.Appl.Phys. vol.48

      Pages: 097003

  • [Journal Article] Label-freeimaging by stimulated parametricemission microscopy reveals adifference in hemoglobin distributionbetween live and fixederythrocytes2009

    • Author(s)
      H. M. Dang, G. Omura, T. Umano, M.Yamagiwa, S. Kajiyama, Y. Ozeki, K.Itoh, K. Fukui
    • Journal Title

      J.Biomed.Opt. vol.14

      Pages: 040506

  • [Journal Article] Analysis andexperimental assessment of thesensitivity of stimulated Ramanscattering microscopy2009

    • Author(s)
      Y. Ozeki, F. Dake, S. Kajiyama, K.Fukui, K. Itoh
    • Journal Title

      Opt.Express vol.17

      Pages: 3651-3658

  • [Journal Article] Highly Sensitive Signal Detectionin Stimulated Parametric EmissionMicroscopy Based on Two-BeamInterferometry2008

    • Author(s)
      M. Yamagiwa, Y. Ozeki, T. Kawasumi, S.Kajiyama, K. Fukui, K. Itoh
    • Journal Title

      Japanese Journal ofApplied Physics vol.47, no. 12

      Pages: 8820-8824

  • [Presentation] 誘導ラマン散乱によるバイオイメージング2011

    • Author(s)
      小関泰之、伊東一良
    • Organizer
      レーザー学会第31回年次大会(10aIX4)
    • Place of Presentation
      電気通信大学
    • Year and Date
      2011-01-10
  • [Presentation] Subharmonic synchronization oftwo-color laser pulses for stimulatedRaman scattering microscopy2010

    • Author(s)
      小関泰之, 北川雄真, 住村和彦, 西澤典彦, 梅村航, 石井万紀子, 梶山慎一郎, 福井希一, 伊東一良
    • Organizer
      CLEO2010, CWD1
    • Place of Presentation
      San Jose
    • Year and Date
      20100500
  • [Presentation] High-sensitivity stimulated Ramanscattering microscopy2010

    • Author(s)
      小関泰之, 伊東一良
    • Organizer
      microCARS2010
    • Place of Presentation
      Bad Honnef
    • Year and Date
      2010-10-18
  • [Presentation] ショット雑音限界感度を有する高調波同期型誘導ラマン散乱顕微鏡2010

    • Author(s)
      小関泰之, 北川雄真, 住村和彦, 西澤典彦, 梅村航, 梶山慎一郎, 福井希一, 伊東一良
    • Organizer
      2010年秋季応用物理学会(16p-D-13)
    • Place of Presentation
      長崎大学
    • Year and Date
      2010-09-16
  • [Presentation] 高調波同期した2波長光源による誘導ラマン散乱顕微鏡の高感度化2010

    • Author(s)
      小関泰之, 北川雄真, 梅村航, 住村和彦, 西澤典彦, 梶山慎一郎, 福井希一, 伊東一良
    • Organizer
      第5回超高速光エレクトロニクス研究会
    • Place of Presentation
      慶応大学日吉キャンパス
    • Year and Date
      2010-08-20
  • [Presentation] High-sensitivity stimulated Ramanscattering (SRS) microscopy based onthe subharmonic synchronizationtechnique2010

    • Author(s)
      小関泰之, 伊東一良
    • Organizer
      LPHYS'10, Seminar 5.6.2,Foz do Iguacu
    • Place of Presentation
      Brazil
    • Year and Date
      2010-07-06
  • [Presentation] 2波長パルス光源の高精度な高調波同期による誘導ラマン散乱顕微鏡2010

    • Author(s)
      小関泰之、北川雄真、住村和彦、西澤典彦、梅村航、石井万紀子、梶山慎一郎、福井希一、伊東一良、
    • Organizer
      2010年春季応用物理学会(19p-F-11)
    • Year and Date
      2010-03-19
  • [Presentation] Biological applications ofnovel nonlinear optical microscopy2009

    • Author(s)
      梶山慎一郎, 小関泰之, 福井希一, 伊東一良
    • Organizer
      The2009Euro American Workshop onInformation Optics Paris
    • Place of Presentation
      France
    • Year and Date
      20090720-20090724
  • [Presentation] 高調波同期した2波長光源による誘導ラマン散乱顕微鏡2009

    • Author(s)
      北川雄真, 小関泰之, 住村和彦, 西澤典彦, 伊東一良
    • Organizer
      Optics &Photonics Japan2009(25pB10)
    • Year and Date
      2009-11-25
  • [Presentation] StimulatedRaman scattering (SRS) microscopy:toward highly sensitive vibrationalimaging in the terahertz regime2009

    • Author(s)
      小関泰之, 伊東一良
    • Organizer
      2ndTopical Problems of Biophotonics 2009
    • Place of Presentation
      Nizhny Novgorod, Russia
    • Year and Date
      2009-07-23
  • [Presentation] StimulatedRaman scattering microscopy forlive-cell imaging with high contrastand high sensitivity2009

    • Author(s)
      小関泰之, 伊東一良
    • Organizer
      18thInternational Laser Physics Workshop(LPHYS'09)(paper 5.6.3)
    • Place of Presentation
      Barcelona,Spain
    • Year and Date
      2009-07-16
  • [Presentation] Background-free,3-D vibrational imaging by stimulatedRaman scattering microscopy2009

    • Author(s)
      小関泰之, 嶽文宏, 梶山慎一郎, 福井希一, 伊東一良
    • Organizer
      Conference on Lasers andElectro-Optics (CLEO2009)(paper CFL2)
    • Place of Presentation
      Baltimore, USA
    • Year and Date
      2009-06-05
  • [Presentation] Experimentalassessment of the sensitivity ofstimulated Raman scatteringmicroscopy2009

    • Author(s)
      嶽文宏, 小関泰之, 梶山慎一郎, 福井希一, 伊東一良
    • Organizer
      the 4th Asian PacificSymposium on Biophotonics (APBP2009)(paper MIC-08)
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2009-05-29
  • [Presentation] 誘導ラマン散乱顕微鏡の感度の実験検討2009

    • Author(s)
      嶽文宏, 小関泰之, 梶山慎一郎, 福井希一, 伊東一良
    • Organizer
      応用物理学会(31a-ZW-9)
    • Year and Date
      2009-03-31
  • [Presentation] 多波長型誘導パラメトリック発光顕微鏡2009

    • Author(s)
      北川雄真, 嶽文宏, 馬野俊幸, 小関泰之, 梶山慎一郎, 福井希一, 伊東一良
    • Organizer
      応用物理学会(31a-ZW-9)
    • Year and Date
      2009-03-31
  • [Presentation] Nonlinear Ultra-fastFocal-point Optics (NUFO) for Biology- Nonlinear Microscopy andManipulation of IntracellularStructures2008

    • Author(s)
      伊東一良
    • Organizer
      LASERLAB ForesightWorkshop and Users Meeting
    • Place of Presentation
      CretaMaris beach hotel, Heraklion, CreteGreece
    • Year and Date
      20081023-20081024
  • [Presentation] 誘導ラマン散乱顕微法の原理確認2008

    • Author(s)
      嶽文宏, 小関泰之, 伊東一良
    • Organizer
      Optics&Photonics Japan 2008(5pC12)
    • Year and Date
      2008-11-05
  • [Presentation] 二光束干渉型誘導パラメトリック発光顕微法を用いた非線形位相イメージング2008

    • Author(s)
      山際将具, 小関泰之, 梶山慎一郎, 福井希一, 伊東一良
    • Organizer
      2008年秋季第69回応用物理学会学術講演会(2p-ZH-14)
    • Place of Presentation
      中部大学
    • Year and Date
      2008-09-02
  • [Presentation] Depth-resolved visualization ofstress-induced anisotropy inside bentoptical fibers by use of four-wavemixing microscopy2008

    • Author(s)
      Y.Ozeki, T.Kawasumi, K.Itoh
    • Organizer
      6thInternational Conference on OpticsDesign and Fabrication (ODF2008)(paper 10S2-12)
    • Year and Date
      2008-06-10
  • [Presentation] Nonlinear ultra-fastfocal-point optics for 3-D microscopyof organic and inorganicsubstances2008

    • Author(s)
      伊東一良
    • Organizer
      6th InternationalConference on Optics Design andFabrication (ODF2008)(paper 9PL-02)
    • Place of Presentation
      Taipei
    • Year and Date
      2008-06-09
  • [Presentation] Characterization ofbending-induced density change insidean optical fiber by use of four-wavemixing microscopy2008

    • Author(s)
      T.Kawasumi, Y.Ozeki, K.Itoh
    • Organizer
      Conference onLasers and Electro-Optics (CLEO2008)(paper JWA19)
    • Year and Date
      2008-05-07
  • [Book] Biological Applications ofStimulated Parametric EmissionMicroscopy and Stimulated RamanScattering Microscopy(Information Optics and Photonics,'Chapter 7, Springer)2011

    • Author(s)
      S. Kajiyama, Y. Ozeki, K. Fukui, K.Itoh
    • Publisher
      (出版予定)
  • [Remarks] ホームページ等

    • URL

      http://www-photonics.mls.eng.osaka-u.ac.jp

  • [Patent(Industrial Property Rights)] 光学顕微鏡2009

    • Inventor(s)
      小関泰之, 伊東一良, 嶽文宏, 福井希一, 梶山慎一郎, 北川悠真, 西澤典彦, 住村和彦
    • Industrial Property Rights Holder
      大阪大学
    • Industrial Property Number
      特願,2009-2655144
    • Filing Date
      2009-11-20
  • [Patent(Industrial Property Rights)] 光学顕微鏡2009

    • Inventor(s)
      小関泰之, 伊東一良, 嶽文宏, 福井希一, 梶山慎一郎
    • Industrial Property Rights Holder
      大阪大学
    • Industrial Property Number
      特願,2009-134364
    • Filing Date
      2009-06-03

URL: 

Published: 2012-01-26   Modified: 2016-04-21  

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