2010 Fiscal Year Final Research Report
Development of the high performance electrofusion device for loading lyo-, cryoprotective agents in vivo
| Project/Area Number |
20360094
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thermal engineering
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SHIRAKASHI Ryo The University of Tokyo, 生産技術研究所, 准教授 (80292754)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKANO Kiyoshi 東京大学, 生産技術研究所, 助教 (60302626)
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| Project Period (FY) |
2008 – 2010
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| Keywords | バイオチップ / 電気融合 / 細胞電気物性 |
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| Research Abstract |
The electrofusion of a mammalian cell and a giant unilamellar vesicle encapsulating lyo-, cryoprotectants has been investigated. In this study, a prototype of electrofusion device allowing the fusion one-on-one pair of cell and GUV has been proposed. In addition, the designing method of electrofusion process by calculating the electric field and membrane potential during electrofusion has been developed.
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