Analysis of the molecular mechanism of the initiation of chromosomal DNA replication in eukaryotes

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20370005
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: National Institute of Genetics
• Principal Investigator: TANAKA Seiji National Institute of Genetics, 細胞遺伝研究系, 助教 (50263314)
• Project Period (FY): 2008 – 2011
• Keywords: DNA複製 / CDK / 細胞周期 / 複製開始

Research Abstract

真核細胞の染色体DNA複製は、一回の細胞分裂周期につき一度だけ過不足なく起きるように厳密な制御を受けている。細胞は、1)S期に染色体DNA複製を開始させる活性と、2)染色体DNAの再複製を阻止する活性を巧妙に組み合わせることで、この制御を成立させている。これらのうち、本研究では「染色体DNA複製の開始反応」を分子レベルで理解することを目指す。具体的には以下の3点について解析を進めてゆく。(1)Sld2,Sld3,Dpb11の役割、(2)Cdc45,GINS複合体の役割、(3)Sld3オーソログの探索。

Research Products

• [Journal Article] Multiple Regulatory Mechanisms to Inhibit Untimely Initiation of DNA Replication Are Important for Stable Genome Maintenance. (2011)
  • Author(s): Tanaka, S., Araki, H.
  • Journal Title: PLoS Genetics in press.
  • Peer Reviewed
• [Journal Article] Regulation of the initiation step of DNA replication by cyclin-dependent kinases. (2010)
  • Author(s): Tanaka, S., Araki, H.
  • Journal Title: Chromoosma 119 Pages: 565-574
  • Peer Reviewed
• [Journal Article] 真核細胞の細胞周期制御機構と染色体DNA複製 (2009)
  • Author(s): 田中誠司、荒木弘之
  • Journal Title: 遺伝 63 Pages: 51-58
• [Journal Article] 真核細胞染色体DNA複製開始反応とCDKによる制御 (2008)
  • Author(s): 田中誠司、荒木弘之
  • Journal Title: 細胞工学 27 Pages: 985-991
• [Presentation] How early-firing origins are determined in budding yeast. (2010)
  • Author(s): Tanaka, S., Araki, H.
  • Organizer: 2010 FASEB Summer Research Conferences "Yeast Chromosome Structure, Replication & Segregation"
  • Place of Presentation: at Carefree, AZ.USA.
  • Year and Date: 20100000
• [Presentation] Cdc7-Dbf4 kinase specifies early replication origins by regulating the association of low-abundant replication initiation factors in budding yeast. (2010)
  • Author(s): Tanaka, S.
  • Organizer: 2010 3R (Replication, Recombination & Repair) Symposium
  • Place of Presentation: at Toyama.
  • Year and Date: 20100000
• [Presentation] Multiple regulatory mechanisms of the initiation of DNA replication are important for stable genome maintenance. (2010)
  • Author(s): Tanaka, S.
  • Organizer: International Conference on Radiation and Cancer Biology at Nagasaki 2010
  • Place of Presentation: at Nagasaki.
  • Year and Date: 20100000
• [Presentation] Multiple regulatory mechanisms of the initiation of DNA replication are important for stable genome maintenance. (2009)
  • Author(s): Tanaka, S., Araki, H.
  • Organizer: Cold Spring Harbor Meeting "Eukaryotic DNA Replication & Genome Maintenance"
  • Place of Presentation: at Cold Spring Harbor, NY.USA.
  • Year and Date: 20090000
• [Presentation] Periodic expression of Sld2 is important for DNA replication and genome integrity. (2008)
  • Author(s): Tanaka, S., Araki, H.
  • Organizer: 2008 FASEB Summer Research Conferences "Yeast Chromosome Structure, Replication & Segregation"
  • Place of Presentation: at Carefree, AZ.USA.
  • Year and Date: 20080000
• [Remarks] ホームページ
  • URL: http://www.nig.ac.jp/labs/MicGen/index.html