Analysis of the molecular mechanism of the initiation of chromosomal DNA replication in eukaryotes

2011 Fiscal Year Final Research Report

• Project/Area Number: 20370005
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: National Institute of Genetics
• Principal Investigator: TANAKA Seiji 国立遺伝学研究所, 細胞遺伝研究系, 助教 (50263314)
• Project Period (FY): 2008 – 2011
• Keywords: DNA複製 / CDK / 細胞周期 / 複製開始

Research Abstract

Chromosomal DNA replication in eukaryotes initiates from multiple origins of replication. Because chromosomal DNA must be precisely duplicated per every cell cycle, cells have regulatory mechanisms that limit the origin activation to once per cell cycle. In this study, we found how cells inhibit the premature untimely initiation of DNA replication and how to coordinate the activation of multiple replication origins.

Research Products

• [Journal Article] Origin-association of Sld3, Sld7 and Cdc45 proteins, specifies the timing of origin-firing (2011)
  • Author(s): Tanaka, S., Katou, Y., Nakato, R., Shirahige, K., and Araki, H.
  • Journal Title: Curr. Biol Volume: 21 Pages: 2055-2063
  • DOI: DOI:10.1016/j.cub.2011.11.038
  • Peer Reviewed
• [Journal Article] Multiple Regulatory Mechanisms to Inhibit Untimely Initiation of DNA Replication Are Important for Stable Genome Maintenance (2011)
  • Author(s): Tanaka, S., and Araki, H.
  • Journal Title: PLoS Genet Volume: 6 Pages: e1002136
  • DOI: DOI:10.1371/journal.pgen.1002136
  • Peer Reviewed
• [Journal Article] Regulation of the initiation step of DNA replication by cyclin-dependent kinases (2010)
  • Author(s): Tanaka, S., and Araki, H.
  • Journal Title: Chromosoma Volume: 119 Pages: 565-574
  • DOI: DOI:10.1007/s00412-010-0291-8
  • Peer Reviewed
• [Presentation] DDK-dependent recruitment of Sld3, Sld7 and Cdc45 specifies the timing of origin-firing in budding yeast (2011)
  • Author(s): Tanaka, S.
  • Organizer: 2011 Cold Sprong Harbor Laboratory Meeting on EUKARYOTIC DNA REPLICATION & GENOME MAINTENANCE
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2011-09-09
• [Presentation] Yeast initiation proteins, Slds3, Sldi7 and Cdc45, specifies the timing of origin-firing (2011)
  • Author(s): Tanaka, S.
  • Organizer: 6th UK-Japan Cell Cycle Workshop
  • Place of Presentation: Lake Windermere, United Kingdom
  • Year and Date: 2011-04-02
• [Presentation] Howearly firingg origins are determined in budding yeast (2010)
  • Author(s): Tanaka, S.
  • Organizer: FASEB Summer Research Conference 'Yeast Chromosome Structure, Replication & Segregation'
  • Place of Presentation: Carefree, AZ, USA
  • Year and Date: 2010-08-11
• [Presentation] Multiple regulatory mechanisms of the initiation of DNA replication are important for stable genome maintenance (2009)
  • Author(s): Tanaka, S.
  • Organizer: Cold Spring Harbor Meeting "Eukaryotic DNA Replication & Genome Maintenance"
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2009-09-02
• [Presentation] Multiple regulatory mechanisms of the initiation of DNA replication are important for stable genome maintenance (2009)
  • Author(s): Tanaka, S.
  • Organizer: Swiss-Japan Cell Cycle Meeting-Chromosome Dynamics and Genome Stability
  • Place of Presentation: Villars-sur-Ollons, Switzerland
  • Year and Date: 2009-05-15
• [Presentation] Periodic expression of Sld2 is important for DNA replication and genome integrity (2008)
  • Author(s): Tanaka, S.
  • Organizer: FASEB Summer Research Conference 'Yeast Chromosome Structure, Replication & Segregation'
  • Place of Presentation: Carefree, AZ, USA
  • Year and Date: 2008-06-25