2010 Fiscal Year Final Research Report
Molecular Mechanism of Nascent Peptide-Mediated Translation Arrest Involved in Feedback Regulation of Methionine Biosynthesis
| Project/Area Number |
20370016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Hokkaido University |
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Principal Investigator |
NAITO Satoshi Hokkaido University, 大学院・農学研究院, 教授 (20164105)
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| Project Period (FY) |
2008 – 2010
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| Keywords | シロイヌナズナ / メチオニン / 翻訳制御 / フィードバック制御 / 新生ペプチド / 試験管内翻訳系 |
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| Research Abstract |
Cystathionine γ-synthase (CGS) catalyzes the first committed step of methionine biosynthesis in plants, and is encoded by CGS1 gene in Arabidopsis. Expression of CGS1 is feedback-regulated at the step of CGS1 mRNA degradation and S-adenosyl-L-methionine (SAM) acts as an effector. We found that, when ribosome translates the CGS1 mRNA in the presence of SAM, either the ribosome itself or the nascent peptide within the ribosomal exit tunnel changes its conformation. We also obtained evidence suggesting that the ribosomal proteins that constitute the exit tunnel is involved in this regulation.
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Research Products
(18 results)
