Molecular mechanism for hydrotropism that plays a role in plant adaptation to arid environment

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20370017
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tohoku University
• Principal Investigator: TAKAHASHI Hideyuki Tohoku University, 大学院・生命科学研究科, 教授 (70179513)
• Project Period (FY): 2008 – 2011
• Keywords: 環境応答 / 水分屈性 / MIZUKUSSEI,オルガネラ / シロイヌナズナ / ヒメツリガネゴケ / キュウリ / オーキシン

Research Abstract

本研究では、植物が乾燥環境適応戦略として獲得した根の水分屈性を制御する分子の同定と機能解析を行い、それら分子のネットワーク機構を明らかにし、これまでに未知であった水分勾配感受とそのシグナル伝達に関するモデルを構築することを目的としている。そのために本研究では、これまでに同定した水分屈性に必須且つ特異的な制御分子であるMIZ1の機能とともに、MIZ2が支配する水分屈性に特異的な小胞輸送系を明らかにする研究を行っている。また、miz1およびmiz2に加えて、これまでに単離することに成功した水分屈性欠損突然変異体miz3の変異原因遺伝子の同定を行うとともに、新規の水分屈性突然変異体を単離し、それらの変異原因遺伝子を同定し、それらがコードするタンパク質の機能を明らかにするための研究を行っている。さらに、これら水分屈性制御分子間の関係を解析することによって、根が水分勾配を感受して伸長方向を決定するまでの分子機構の全体像を明らかにするための基盤研究を行っている。

Research Products

• [Journal Article] Transcriptome analysis of gene expression during the hydrotropic response in Arabidopsis seedlings. (2010)
  • Author(s): Moriwaki T, Miyazawa Y, Takahashi H
  • Journal Title: Environmental and Experimental Botany 69 Pages: 148-157
  • Peer Reviewed
• [Journal Article] Auxin reverses plant male sterility caused by high tempertures. (2010)
  • Author(s): Sakata T, Oshino T, Miura S, Tomabechi M, Tsunaga Y, Higashitani N, Miyazawa Y, Takahashi H, Watanabe M, Higashitani A
  • Journal Title: PNAS 107 Pages: 8569-8574
  • Peer Reviewed
• [Journal Article] GNOM-mediated vesicular trafficking plays an essential role in hydrotropism of Arabidopsis roots. (2009)
  • Author(s): Miyazawa Y, Takahashi A, Kobayashi A, Kaneyasu T, Fujii N, Takahashi H
  • Journal Title: Plant Physiol 149 Pages: 835-840
  • Peer Reviewed
• [Journal Article] Hormonal interactions during root tropic growth: hydrotropism versus gravitropism. (2009)
  • Author(s): Takahashi H, Miyazawa Y, Fujii N
  • Journal Title: Plant Mol Biol 69 Pages: 489-502
  • Peer Reviewed
• [Journal Article] Effects of locally targeted heavy-ion and laser microbeam on root hydrotropism in Arabidopsis thaliana. (2008)
  • Author(s): Miyazawa Y, Sakashita T, Funayama T, Hamada N, Negishi H, Kobayashi A, Kaneyasu T, Ooba A, Morohashi K, Kakizaki T, Wada S, Kobayashi Y, Fujii N, Takahashi H
  • Journal Title: J Rad Res 49 Pages: 373-379
  • Peer Reviewed
• [Presentation] Biochemical analysis of MIZ1, an essential protein for hydrotropism in Arabidopsis thaliana. (2010)
  • Author(s): Yamazaki T, Kobayashi A, Miyazawa Y, Takahashi H
  • Organizer: Plant Biology 2010
  • Place of Presentation: Montreal, Canada.
  • Year and Date: 20100731-20100804
• [Presentation] Hormonal regulation of lateral root development is modulated by overexpression of MIZ1. (2010)
  • Author(s): Moriwaki T, Miyazawa Y, Kobayashi A, Uchida M, Fujii N, Takahashi H
  • Organizer: 21^<st> International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama
  • Year and Date: 20100606-20100610
• [Presentation] Identification of genes responsible for root hydrotropism in Arabidopsis roots. (2009)
  • Author(s): Miyazawa Y, Fujii N, Takahashi H
  • Organizer: Plant Biology 2009.
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, USA.
  • Year and Date: 20090718-20090722
• [Presentation] Genetic evidence for mechanisms unique to hydrotropism but independent of gravitropism in Arabidopsis roots. (2009)
  • Author(s): Takahashi H, Miyazawa Y, Kobayashi Y, Moriwaki T, Uchida M, Fujii N
  • Organizer: International Symposium on Plant Sensing, Response and Adaptation to Altered Water Status.
  • Place of Presentation: Taipei, Taiwan.
  • Year and Date: 20090312-20090313
• [Presentation] Molecular mechanisms responsible for interaction or differentiation between hydrotropism and gravitropism in roots. (2008)
  • Author(s): Morohashi K, Kobayashi A, Miyazawa Y, Fujii N, Takahashi H
  • Organizer: 37^<th> COSPAR Scientific Assembly.
  • Place of Presentation: Montreal, Canada.
  • Year and Date: 20080713-20080720
• [Presentation] Mechanisms unique to hydrotropism in seedling roots. (2008)
  • Author(s): Takahashi H, Miyazawa Y, Fujii N
  • Organizer: 4^<th> Plant Neurobiology Conference.
  • Place of Presentation: Fukuoka
  • Year and Date: 20080406-20080609