2011 Fiscal Year Final Research Report
Analysis of molecular mechanism for root hydrotropism that plays a role in plant adaptation to drought conditions
| Project/Area Number |
20370017
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUJII Nobuharu 東北大学, 大学院・生命科学研究科, 准教授 (70272002)
MIYAZAWA Yutaka 東北大学, 大学院・生命科学研究科, 助教 (00342858)
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| Project Period (FY) |
2008 – 2011
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| Keywords | 環境応答 |
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| Research Abstract |
We attempted to reveal molecular mechanism of hydrotropism in Arabidopsis roots by analyzing the functions of MIZ1 and MIZ2 that play essential roles in hydrotropism. First, we generated the overexpressors of MIZ1 (MIZ1OEs)and analyzed their phenotypes. We found that MIZ1OEs showed an enhanced hydrotropism and a reduced lateral root formation. These phenomena likely resulted form the decreased level of auxin content in MIZ1OEs. Also, it was shown that the miz2 mutation is epistatic to MIZ1 overexpression. MIZ1-GFP introduced into miz1 mutant complemented the mutant phenotype and was detected at high levels in cortical cells and lateral root cap cells. MIZ1-GFP was fractionated into a soluble protein fraction and an endoplasmic reticulum (ER)membrane fraction, where it was bound to the surface of the ER membrane at the cytosolic side. Furthermore, we succeeded in isolating proteins that bind MIZ1 by immunoprecipitation method and LC-MS/MS analysis. In addition, we obtained mutants of suppressors of both miz1 and miz2. This study showed that light and ABA signaling are independently integrated into MIZ1 expression and regulate hydrotropism. Then, we demonstrated that the MIZ1-regulated hydrotropism of lateral roots plays an important role in root system development in soil and contributes to drought avoidance, which results in better survival and yield under the water-limited conditions.
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[Journal Article] Gravistimulation changes the accumulation pattern of the CsPIN1 auxin efflux facilitator in the endodermis of the transition zone in cucumber seedlings.2012
Author(s)
Watanabe C, Fujii N, Yanai K, Hotta T, Kim D-H, Kamada M, Sasagawa-Saito Y, Nishimura T, Koshiba T, Miyazawa Y, Kim K-M, Takahashi H
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Journal Title
Plant Physiology
Volume: 158
Pages: 239-251
Peer Reviewed
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[Journal Article] Effects of locally targeted heavy-ion and laser microbeam on root hydrotropism in Arabidopsis thaliana2008
Author(s)
Miyazawa Y, Sakashita T, Funayama T, Hamada N, Negishi H, Kobayashi A, Kaneyasu T, Ooba A, Morohashi K, Kakizaki T, Wada S, Kobayashi Y, Fujii N, Takahashi H
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Journal Title
Journal of Radiation Research
Volume: 49
Pages: 373-379
Peer Reviewed
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[Presentation] Two spaceflight expreriments, Hydro Tropi and CsPINs, for the study of hydrotropism in seedling roots.2011
Author(s)
H. Takahashi, M. Okamoto, K. Morohashi, C. Watanabe, Y. Miyazawa, N. Fujii, T. Yamazaki, A. Higashibata, N. Ishioka, T. Shimazu, Y. Fusejima, H. Kasahara, M. Kamada.
Organizer
The 28th International Symposium on Space Technology and Science (ISTS
Place of Presentation
Okinawa
Year and Date
20110605-12
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