2010 Fiscal Year Final Research Report
Structure and Function of Peroxins Essential for Peroxisome Assembly
| Project/Area Number |
20370039
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
FUJIKI Yukio Kyushu University, 大学院・理学研究院, 教授 (70261237)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Shigehiko 九州大学, 大学院・理学研究院, 准教授 (90236753)
MIKI Kunio 京都大学, 大学院・理学研究科, 教授 (10116105)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ペルオキシソーム / CHO変異細胞 / ペルオキシン / タンパク質の輸送・局在化機構 / AAA ATPase / タンパク質相互作用 / レドックスポテンシャル / PPAR |
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| Research Abstract |
Pex14p is a central component of the peroxisomal matrix protein import machinery. We successfully delineated the crystal structure at 1.8 A resolution of the conserved domain comprising the amino-acid residues at 25-70 of mammalian Pex14p. A hydrophobic surface is composed of the conserved residues, including the protruding Phe35 and Phe52, and forms two binding pockets for biding the helical WXXXF/Y motif of Pex5p. As to peroxisomal membrane assembly, we demonstrated that Pex16p functions as the Pex3p-docking site and serves as the peroxisomal membrane receptor that is specific to the Pex3p-Pex19p complexes, termed the class II pathway.
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[Journal Article] A novel fluorescent sensor protein for visualization of redox states in the cytoplasm and in peroxisomes.2010
Author(s)
Yano, T., Oku, M., Akeyama, N., Itoyama, A., Yurimoto, H., Kuge, S., Fujiki Y., Sakai, Y.
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Journal Title
Mol.Cell.Biol. 30
Pages: 3758-3766
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[Journal Article] Rcruitment of the RNA helicase RHAU to stress granules via a unique RNA-binding domain.2008
Author(s)
Chalupnikova, K., Lattmann, S., Selak, N., Iwamoto, F., Fujiki Y., Nagamine, Y.
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Journal Title
J.Biol.Chem. 283
Pages: 35186-35198
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[Journal Article] Characterization of the interaction between recombinant human peroxin PEX3p and PEX19p : Identification of TRP104 in Pex3p as a critical residue for the interaction.2008
Author(s)
Sato, Y., Shibata, H., Nakano, H., Matsuzono, Y., Yoshinori, K., Kobayashi, Y., Fujiki Y., Imanaka, T., Kato, H.
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Journal Title
J.Biol.Chem. 283
Pages: 6136-6144
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