2010 Fiscal Year Final Research Report
X-ray crystallographic analysis of microsomal prostaglandin E2synthase1
| Project/Area Number |
20370042
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
AGO Hideo The Institute of Physical and Chemical Research, 宮野構造生物物理研究室, 専任研究員 (70360477)
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| Project Period (FY) |
2008 – 2010
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| Keywords | アラキドン酸カスケード / 膜タンパク質 / X線結晶構造解析 / プロスタグランジンE2 |
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| Research Abstract |
Human membrane protein microsomal prostaglandin E2 synthase 1(mPGES1) was overexpressed using Schizosaccharomyces pombe. The purification method was established. The recombinant mPGES1 was free from the post-translational modification except for the deletion of the N-terminal methionine residue, and had a higher specific activity. The recombinant mPGES1 was crystallized in the two crystallization conditions.
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Research Products
(6 results)
