Physiological and pathological roles of CD47-SHPS-1 system

2010 Fiscal Year Final Research Report

• Project/Area Number: 20370044
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Gunma University
• Principal Investigator: MATOZAKI Takashi Gunma University, 生体調節研究所, 客員教授 (80252782)
• Co-Investigator(Kenkyū-buntansha): OHNISHI Hiroshi 群馬大学, 生体調節研究所, 准教授 (70334125)
• Project Period (FY): 2008 – 2010
• Keywords: シグナル伝達学 / 生体分子学 / 蛋白質 / 脳・神経 / 免疫学

Research Abstract

SHPS-1 and CD47 are transmembrane proteins and they constitute a cell-cell communication system by interacting their ectodomains. In this research project, we have shown that CD47-SHPS-1 system participates in regulation of brain stress response and depression-like behavior as well as induction of IL-17-producing helper T cells and autoimmune diseases. In addition, it also plays an important role in regulation of insulin secretion from pancreatic βcells.

Research Products

• [Journal Article] Requirement of SIRPa for protective immunity against leishmania major. (2010)
  • Author(s): Morimoto N., Murata Y., Motegi S., Suzue K..Saito Y..Okazawa H., Ohnishi H., Kotani T., Kusakari S., Ishikawa O., Matozaki T.
  • Journal Title: Biochem.Biophys.Res.Commun. 401 Pages: 385-389
  • Peer Reviewed
• [Journal Article] Role of SIRPa in regulation of mucosal immunity in the intestine. (2010)
  • Author(s): Kanazawa Y., Saito Y., Supriatna Y., Tezuka H., Kotani T., Murata Y., Okazawa H., Ohnishi H., Kinouchi Y., Nojima Y., Ohteki T., Shimosegawa T., Matozaki T.
  • Journal Title: Genes Cells 15 Pages: 1189-1200
  • Peer Reviewed
• [Journal Article] Regulation by SIRPa of dendritic cell homeostasis in lymphoid tissues. (2010)
  • Author(s): Saito Y., Iwamura H., Kaneko T., Ohnishi H., Murata Y., Okazawa H., Kanazawa Y., Sato-Hashimoto M., Kobayashi H., Oldenborg P.-A., Naito M., Kaneko Y., Nojima Y., Matozaki T.
  • Journal Title: Blood 116 Pages: 3517-3525
  • Peer Reviewed
• [Journal Article] Stress-evoked tyrosine phosphorylation of SIRPa regulates behavioral immobility in the forced swim test. (2010)
  • Author(s): Ohnishi H., Murata T., Kusakari S., Hayashi Y., Takao K., Maruyama T., Ago Y., Koda K., Jin F.-J., Okawa K., Oldenborg P.-A., Okazawa H., Murata Y., Furuya N., Matsuda T., Miyakawa T., Matozaki T.
  • Journal Title: J.Neuroscl. 30 Pages: 10472-10483
  • Peer Reviewed
• [Journal Article] Functions and molecular mechanisms of the CD47-SIRPa signalling pathway. (2009)
  • Author(s): Matozaki T., Murata Y., Okazawa H., Ohnishi H.
  • Journal Title: Trends Cell Biol.(Review) 19 Pages: 72-80
  • Peer Reviewed
• [Journal Article] Inhibitory regulation of osteoclast bone resorption by SIRPa. (2009)
  • Author(s): van Beek E.M., de Vries T.J., Mulder L., Schoenmaker T., Hoeben K.A., Matozaki T., Langenbach G.E.J., Kraal G., Everts V., van den Berg T.K.
  • Journal Title: FASEB J. 23 Pages: 4081-4090
  • Peer Reviewed
• [Journal Article] Expression of Src homology 2 domain-containing protein tyrosine phosphatase substrate-1 in pancreatic β-cells and its roles in promotion of insulin secretion and protection against diabetes. (2008)
  • Author(s): Kobayashi M., Ohnishi H., Okazawa H., Murata Y., Hayashi Y., Kobayashi H., Kitamura T., Matozaki T.
  • Journal Title: Endocrinology 149 Pages: 5662-5669
  • Peer Reviewed
• [Journal Article] Resistance to collagen-induced arthritis in SHPS-1 mutant mice. (2008)
  • Author(s): Okuzawa C., Kaneko Y., Murata Y., Miyake A., Saito Y., Okajo J., Tomizawa T., Kaneko Y., Okazawa H., Ohnishi H., Matozaki T., Nojima Y.
  • Journal Title: Biochem. Biophys.Res.Commun. 371 Pages: 561-566
  • Peer Reviewed
• [Journal Article] Negative regulation by SHPS-1 of Toll-like receptor-dependent proinflammatory cytokine production in macrophages. (2008)
  • Author(s): Miyake A., Murata Y., Okazawa H., Ikeda H., Niwayama Y., Ohnishi H., Hirata Y., Matozaki T.
  • Journal Title: Genes Cells 13 Pages: 209-219
  • Peer Reviewed
• [Journal Article] Trans-endocytosis of CD47 and SHPS-1 and its role in regulation of the CD47-SHPS-1 system. (2008)
  • Author(s): Kusakari S., Ohnishi H., Jin F.-J., Kaneko Y., Murata T., Murata Y., Okazawa H., Matozaki T.
  • Journal Title: J.Cell Sci. 121 Pages: 1213-1223
  • Peer Reviewed
• [Presentation] Stress-evoked tyrosine phosphorylation of SIRPa mediates an antidepressant effect. (2010)
  • Author(s): Ohnishi H., Kusakari S., Murata T., Hayashi Y., Maruyama T., Okazawa H., Murata Y., Takao K., Miyakawa T., Oldenborg P.-A., Matozaki T.
  • Organizer: 40th Annual Meeting of Society for Neuroscience.
  • Place of Presentation: San Diego Convention Center (USA, San Diego)
  • Year and Date: 20101113-20101117
• [Presentation] チロシンリン酸化シグナルによる脳のストレス応答制御 (2010)
  • Author(s): 大西浩史、草苅伸也、村田考啓、丸山登士、林由里子、高雄啓三、宮川剛、吾郷由希夫、香田健、松田敏夫、大川克也、齊藤泰之、村田陽二、的崎尚
  • Organizer: 第33回日本神経科学大会、第53回日本神経化学会大会、第20回日本神経回路学会大会、合同大会
  • Place of Presentation: 兵庫
  • Year and Date: 20100902-20100924
• [Presentation] Regulation by SIRPa of homeostasis of lymphoid tissue dendritic cells. (2010)
  • Author(s): Saito Y., Iwamura H., Kaneko T., Kanazawa Y., Sato-Hashimoto M., Murata Y., Ohnishi H., Kaneko Y., Nojima, Y., Matozaki T.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 20100822-20100827
• [Presentation] CD47-SIRPα系による免疫機能制御 (2009)
  • Author(s): 齋藤泰之、岩村紘子、金澤義丈、橋本美穂、村田陽二、大西浩史、金子和光、野島美久、的崎尚
  • Organizer: 第71回日本血液学会
  • Place of Presentation: 京都
  • Year and Date: 20091023-20091025
• [Presentation] Roles of the CD47-SIRPa signaling in homeostasis of lymphoid tissue dendritic cells and development of autoimmune diseases. (2009)
  • Author(s): Matozaki T., Saito Y., Iwamura H., Kanazawa Y., Sato-Hashimoto M., Murata Y., Ohnishi H., Kaneko Y., Nojima Y.
  • Organizer: G-COE Program International Symposium
  • Place of Presentation: 秋田大学(秋田)
  • Year and Date: 20090624-20090625
• [Presentation] 腸管におけるSHPS-1発現と腸管免疫制御 (2008)
  • Author(s): 金澤義丈、齋藤泰之、ヤナ・スプリアトナ、村田陽二、岡澤秀樹、大西浩史、下瀬川透、的崎尚
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 20081201-20081203
• [Presentation] SHPS-1は樹状細胞の恒常性に重要である (2008)
  • Author(s): 齋藤泰之、金子和光、岩村紘子、金澤義丈、村田陽二、大西浩史、野島美久、的崎尚
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 20081201-20081203
• [Presentation] Expression of SHPS-1 in pancreatic β-cells and its role in promotion of insulin secretion and protection against diabetes. (2008)
  • Author(s): Kobayashi M., Ohnishi H., Okazawa H.Murata Y., Hayashi Y., Kobayashi H.Kitamura T., Matozaki T.
  • Organizer: 第8回国際プロテインホスファターゼカンファレンス
  • Place of Presentation: 前橋テルサ(群馬)
  • Year and Date: 20081112-20081114
• [Presentation] Trans-endocytosis of CD47 and SHPS-1 and its role in regulation of the CD47-SHPS-1 system. (2008)
  • Author(s): Kusakari S., Ohnishi H., Okazawa H., Murata T., Murata Y., Matozaki T.
  • Organizer: 第8回国際プロテインホスファターゼカンファレンス
  • Place of Presentation: 前橋テルサ(群馬)
  • Year and Date: 20081112-20081114
• [Presentation] チロシンポスファターゼによるがん、炎症性腸炎の制御 (2008)
  • Author(s): 的崎尚、岡澤秀樹、村田陽二、大西浩史
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知)
  • Year and Date: 20081028-20081030
• [Presentation] Essential roles of SHPS-1 in homeo-stasis of dendritic cells. (2008)
  • Author(s): 齋藤泰之、金子和光、岩村紘子、金澤義丈、村田陽二、大西浩史、野島美久、的崎尚
  • Organizer: 第10回国際樹状細胞シンポジウム
  • Place of Presentation: 神戸国際会議場(兵庫)
  • Year and Date: 20081001-20081005
• [Presentation] CD47とSHPS-1のトランスエンドサイトーシスとCD47-SHPS-1系の制御における役割 (2008)
  • Author(s): 大西浩史、草苅伸也、村田考啓、村田陽二、岡澤秀樹、的崎尚
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 20080629-20080701
• [Presentation] 受容体型膜蛋白質SHPS-1のインスリン分泌制御における機能解析 (2008)
  • Author(s): 小林雅樹、大西浩史、岡澤秀樹、村田陽二、林由里子、北村忠弘、的崎尚
  • Organizer: 第51回日本糖尿病学会年次学術集会
  • Place of Presentation: 東京国際フォーラム(東京)
  • Year and Date: 20080522-20080524
• [Book] シグナル伝達研究(2008-'09 Vo1.26 No.15)
  • Author(s): 的崎尚、岡田雅人、他
  • Total Pages: 248
  • Publisher: 羊土社
• [Remarks] ホームページ等
  • URL: http://www.imcr.gunma-u.ac.jp/lab/biosig/
• [Remarks] ホームページ等
  • URL: http://www.med.kobe-u.ac.jp/tougou/signall/index.html