2010 Fiscal Year Final Research Report
Analysis of physiological function of su l fat i on by regulating the expression of PAPS transporter
Project/Area Number |
20370051
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Functional biochemistry
|
Research Institution | Soka University |
Principal Investigator |
NISHIHARA Shoko Soka University, 工学部・生命情報工学科 (00164575)
|
Co-Investigator(Renkei-kenkyūsha) |
KUDO Takashi 筑波大学, 人間総合科学研究科, 准教授 (20288062)
TOYODA Hidenao 立命館大学, 薬学部, 教授 (70217579)
ONO Masaya 国立がんセンター, 化学療法部, 室長 (00270900)
|
Project Period (FY) |
2008 – 2010
|
Keywords | 糖鎖生物学 / 硫酸化 / PAPs輸送体 / ノックアウトマウス / ES細胞 / RNAi / ノックダウン / 生理機能 |
Research Abstract |
Sulfation of glycans, lipids, and proteins is involved in a variety of biological phenomena. PAPS transporters (PAPSTs) is a key component of sulfation processes. We performed RNAi knockdown for PAPSTs in mouse ES cells and found that sulfated glycans work on the maintenance and differentiation of ES cells through Wnt, BMP and FGF signals. PAPST1 knockout mice showed that reduction of PAPST activity causes various type of disorders.
|
-
[Journal Article] Expression and role of 3'-phosphoadenosine 5'-phosphosulfate transporters in human colorectal carcinoma.2011
Author(s)
Kamiyama S, Ichimiya T, Ikehara Y, Takase T, Fujimoto I, Suda T, Nakamori S, Nakamura M, Nakayama F, Irimura T, Nakanishi H, Watanabe M, Narimatsu N, Nishihara S
-
Journal Title
Glycobiology 21
Pages: 235-246
-
-
[Journal Article] Two Golgi-resident 3'-phosphoadenosine 5'-phosphosulfate transporters play distinct roles in heparan sulfate modifications and embryonic and larval development in Caenorhabditis elegans.2010
Author(s)
Dejima K, Murata D, Mizuguchi S, Nomura KH, Izumikawa T, Kitagawa H, Gengyo-Ando K, Yoshina S, Ichimiya T, Nishihara S, Mitani S, Nomura K
-
Journal Title
J Biol.Chem. 285
Pages: 24717-24728
-
[Journal Article] The 3'-phosphoadenosine 5'-phosphosulfate transporters, PAPST1 and 2, contribute to the maintenance and differentiation of mouse embryonic stem cells.2009
Author(s)
Sasaki N, Hirano T, Ichimiya T, Wakao M, Hirano K, Kinoshita-Toyoda A, Tovoda H, Suda Y, Nishihara S
-
Journal Title
-
[Journal Article] Heparan sulfate regulates self-renewal and pluripotency of embryonic stem cells.2008
Author(s)
Sasaki N, Okishio K, Ui-Tei K, Saigo K, Kinoshita-Toyoda A, Tovoda H, Nishimura T, Suda Y, Hayasaka M, Hanaoka K, Hitoshi S, Ikenaka K, Nishihara S
-
Journal Title
J.Biol.Chem. 283
Pages: 3594-3606
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-