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2010 Fiscal Year Final Research Report

Structural and functional study of enzymes which are related to central nervous system disorders with muscle deficiency

Research Project

  • PDF
Project/Area Number 20370053
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionHigh Energy Accelerator Research Organization

Principal Investigator

KATO Ryuichi  High Energy Accelerator Research Organization, 物質構造科学研究所, 准教授 (50240833)

Co-Investigator(Kenkyū-buntansha) ENDO Tamao  地独東京都健康長寿医療センター, 東京都健康長寿医療センター研究所, 研究部長 (30168827)
KAWASAKI Masato  高エネルギー加速器研究機構, 物質構造科学研究所, 助教 (00342600)
Project Period (FY) 2008 – 2010
Keywords糖鎖生物学
Research Abstract

We elucidated PomT1 and T2 require sugar modification on their proteins for the activity. We also showed that mechanism of PomT1-T2 complex formation is different between human and yeast but similar between human and zebra-fish. Over expression and purification of PomGnT1 protein for X-ray structural determination was successful by wheat germ cell free expression system. Over expression and purification of fukutin and FKRP proteins were also successful by E. coli expression system.

  • Research Products

    (15 results)

All 2010 2009 2008

All Journal Article (2 results) Presentation (13 results)

  • [Journal Article] Role of N-glycans in maintaining the activity of protein O-mannosyltransferases POMT1 and POMT2.2010

    • Author(s)
      Hiroshi Manya, Keiko Akasaka-Manya, Ai Nakajima, Masao Kawakita, Tamao Endo
    • Journal Title

      J.Biochem. 147(3)

      Pages: 337-344

  • [Journal Article] Protein O-mannosylation is necessary for normal embryonic development in zebrafish.2010

    • Author(s)
      Eriko Avsar-Ban, Hisayoshi Ishikawa, Hiroshi Manya, Masatoki Watanabe, Shinichi Akiyama, Hideo Miyake, Tamao Endo, Yutaka Tamaru
    • Journal Title

      Glycobiology 20(9)

      Pages: 1089-102

  • [Presentation] POMGnT1の基質特異性の解析2010

    • Author(s)
      赤阪啓子、萬谷博、水野真盛、稲津敏行、遠藤玉夫
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20101207-20101210
  • [Presentation] ゼブラフィッシュ初期発生過程においてO-マンノース型糖鎖修飾が必要である2010

    • Author(s)
      アヴシャル-坂恵利子、石川文啓、萬谷博、渡部正利喜、秋山真一、三宅英雄、遠藤玉夫、田丸浩
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20101207-20101210
  • [Presentation] Investigation the role of protein O-mannosylation during development.2010

    • Author(s)
      Tamao Endo, Eriko Avsar-Ban, Hiroshi Manya, Yutaka Tamaru
    • Organizer
      Annual Conference of the Society for Glycobiology
    • Place of Presentation
      St Pete Beach, FL, USA
    • Year and Date
      20101107-20101110
  • [Presentation] Role of N-glycans on protein O-mannosyltransferases POMT1 and POMT2.2010

    • Author(s)
      Hiroshi Manya, Keiko Akasaka-Manya, Tamao Endo
    • Organizer
      XXV International Carbohydrate Symposium
    • Place of Presentation
      Tokyo
    • Year and Date
      20100801-20100806
  • [Presentation] ヒトO-マンノース転移酵素におけるN型糖鎖修飾の解析2010

    • Author(s)
      萬谷博、赤阪-萬谷啓子、遠藤玉夫
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      20100327-20100330
  • [Presentation] O-マンノース型糖鎖修飾と病態2009

    • Author(s)
      遠藤玉夫,萬谷博
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20091209-20091212
  • [Presentation] Role on N-glycans on protein O-mannosyltransferases POMT1 and POMT2.2009

    • Author(s)
      Hiroshi Manya, Keiko Akasaka-Manya, Tamao Endo
    • Organizer
      Austria/Japan Seminar on Comparative Glycobiology and Developmental Biology
    • Place of Presentation
      Hayama, Japan
    • Year and Date
      20090921-20090922
  • [Presentation] ゼブラフィッシュにおけるPOMTおよびPOMGnT1によるO-mannosylation2009

    • Author(s)
      坂恵利子、萬谷博、三宅英雄、遠藤玉夫、田丸浩
    • Organizer
      日本農芸化学会2009年度大会
    • Year and Date
      20090327-20090329
  • [Presentation] O-マンノース転移酵素におけるN型糖鎖の役割2008

    • Author(s)
      萬谷博、赤阪-萬谷啓子、遠藤玉夫
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      20081209-20081212
  • [Presentation] ジストログリカンの糖鎖修飾と筋ジストロフィー2008

    • Author(s)
      遠藤玉夫、萬谷博
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      20081209-20081212
  • [Presentation] 培養細胞を用いたゼブラフィッシュPOMT遺伝子の発現とその酵素活性の検討2008

    • Author(s)
      坂恵利子、萬谷博、遠藤玉夫、田丸浩
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      20081209-20081212
  • [Presentation] O-マンノース転移酵素におけるN型糖鎖の役割2008

    • Author(s)
      萬谷博、赤阪-萬谷啓子、遠藤玉夫
    • Organizer
      第28回日本糖質学会年会
    • Place of Presentation
      つくば
    • Year and Date
      20080818-20080820
  • [Presentation] 神経移動障害を伴う先天性筋ジストロフィー2008

    • Author(s)
      遠藤玉夫、萬谷博、赤阪-萬谷啓子
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京
    • Year and Date
      20080709-20080711

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Published: 2012-01-26   Modified: 2016-04-21  

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