2010 Fiscal Year Final Research Report
Structural and functional study of enzymes which are related to central nervous system disorders with muscle deficiency
| Project/Area Number |
20370053
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | High Energy Accelerator Research Organization |
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Principal Investigator |
KATO Ryuichi High Energy Accelerator Research Organization, 物質構造科学研究所, 准教授 (50240833)
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| Co-Investigator(Kenkyū-buntansha) |
ENDO Tamao 地独東京都健康長寿医療センター, 東京都健康長寿医療センター研究所, 研究部長 (30168827)
KAWASAKI Masato 高エネルギー加速器研究機構, 物質構造科学研究所, 助教 (00342600)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 糖鎖生物学 |
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| Research Abstract |
We elucidated PomT1 and T2 require sugar modification on their proteins for the activity. We also showed that mechanism of PomT1-T2 complex formation is different between human and yeast but similar between human and zebra-fish. Over expression and purification of PomGnT1 protein for X-ray structural determination was successful by wheat germ cell free expression system. Over expression and purification of fukutin and FKRP proteins were also successful by E. coli expression system.
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