2010 Fiscal Year Final Research Report
Molecular basis underlying the generation of spatiotemporal patterns of cytosolic calcium dynamics
| Project/Area Number |
20370054
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MICHIKAWA Takayuki The Institute of Physical and Chemical Research, 発生神経生物研究チーム, 副チームリーダー (90282516)
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| Project Period (FY) |
2008 – 2010
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| Keywords | カルシウムシグナル / 細胞内情報伝達 / セカンドメッセンジャー / イノシトール三リン酸 / カルシウム放出チャネル / カルシウムポンプ / ホスフォリパーゼC / カルシウム振動 |
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| Research Abstract |
The gating mechanism of inositol 1,4,5-trisphosphate receptor (IP_3R)/Ca^<2+> release channels and the Ca^<2+> pump activity of sarco/endoplasmic reticulum Ca^<2+> ATPase during Ca^<2+> oscillations were investigated by using the fluorescent resonance energy transfer technique. Phospholipase C β1 and β4 were identified to be crucial for the generation of Ca^<2+> oscillations in HeLa cells stimulated with histamine. We constructed a mathematical model to reproduce Ca^<2+> and IP_3 dynamics observed and found that a dual-ligand competition of IP_3R is a fundamental mechanism for the generation of Ca^<2+> oscillations.
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[Journal Article] Spontaneous network activity visualized by ultrasensitive Ca^<2+> indicator, yellow Cameleon-Nano2010
Author(s)
Hirokawa K., Yamada Y., Matsuda T., Kobayashi K., Hashimoto M., Matsu-ura T., Miyawaki A., Michikawa T., Mikoshiba K., Nagai T.
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Journal Title
Nat.Methods. 7
Pages: 729-732
Peer Reviewed
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