2010 Fiscal Year Final Research Report
The second ATP-binding site of the chaperonin GroEL and its functional role
| Project/Area Number |
20370066
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | National Institutes of Natural Sciences Okazaki Research Facilities |
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Principal Investigator |
KUWAJIMA Kunihiro National Institutes of Natural Sciences Okazaki Research Facilities, 岡崎統合バイオサイエンスセンター, 教授 (70091444)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 分子シャペロン / シャペロニン / ATP結合 / アロステリック転移 |
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| Research Abstract |
The Escherichia coli chaperonin GroEL, a molecular chaperone that mediates protein folding in the biological cell, expresses its chaperone function through ATP binding. In the present study, we investigated the ATP binding to GroEL by photo-affinity labeling, amino acid sequence analysis, stopped-flow fluorescence spectroscopy, and isothermal titration calorimetry. We found (1) that the second ATP-binding site is present and located near Tyr360, and (2) that in the absence of potassium ion, only the ATP binding to the primary site was observed, and its binding constant is an order of 10^4M^<-1>.
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[Journal Article] Adaptation of a hyperthermophilic group II chaperonin to relatively moderate temperatures.2010
Author(s)
Kanzaki, T., Ushioku, S., Nakagawa, A., Oka, T., Takahashi, K., Nakamura T., Kuwajima K. Yamagishi, A. Yohda, M.
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Journal Title
Protein Eng.Des.Sel. 23
Pages: 393-402
Peer Reviewed
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[Journal Article] Non-native-helix formation is not necessary for folding of lipocalin : Comparison of burst-phase folding between tear lipocalin and β-lactoglobulin.2009
Author(s)
Tsukamoto, S., Yamashita, T., Yamada, Y., Fujiwara, K., Maki, K., Kuwajima K., Matsumura, Y., Kihara, H., Tsuge, H. Ikeguchi, M.
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Journal Title
Proteins 76
Pages: 226-236
Peer Reviewed
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[Journal Article] Asymmetry of the GroEL-GroES complex under physiological conditions as revealed by small-angle X-ray scattering.2008
Author(s)
Inobe, T., Takahashi, K., Maki, K., Enoki, S., Kamagata, K., Kadooka, A., Arai, M. Kuwajima K.
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Journal Title
Biophys.J. 94
Pages: 1392-1402
Peer Reviewed
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[Journal Article] Sequential action of ATP-dependent subunit conformational change and interaction between helical protrusions in the closure of the built-in lid of group II chaperonins.2008
Author(s)
Kanzaki, T., Iizuka, R., Takahashi, K., Maki, K., Masuda, R., Sahlan, M., Yebenes, H., Valpuesta, J.M., Oka, T., Furutani, M., Ishii, N., Kuwajima K., Yohda, M.
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Journal Title
J.Biol.Chem. 283
Pages: 34773-34784
Peer Reviewed
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[Book] A.Experimental and Simulation Studies of the Folding/Unfolding of Goat α-Lactalbumin In : Water and Biomolecules-Physical Chemistry of Life Phenomena. (Kuwajima.K, Goto.Y, Hirata.F, Kataoka.M.& Terazima. M, Eds.)2009
Author(s)
Kuwajima, K., Oroguchi, T., Nakamura, T., Ikeguchi, M. Kidera
Total Pages
13-35
Publisher
Springer-Verlag.
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