2010 Fiscal Year Final Research Report
Small nuclear RNA-mediated mechanism to diversify the genetic information
| Project/Area Number |
20370075
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
HIROSE Tetsurou National Institute of Advanced Industrial Science and Technology, バイオメディシナル情報研究センター, 研究チーム長 (30273220)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 遺伝子発現 / 非コードRNA / RNAプロセシング / RNA修飾 / 細胞核 |
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| Research Abstract |
To explore the function of orphan snoRNAs that are localized in mammalian cell nucleus, we succeeded in optimization of nuclear RNA knockdown using chemically-modified antisense oligonucleotides. The site-specific RNA methylation activity of o-snoRNP was demonstrated using RNA polymerase I-driven reporter construct. The putative targets of o-snoRNAs were identified from the data of RNA-seq analysis using the RNA samples prepared from o-snoRNA-depleted cells. The novel RNA species were identified from the RNA-seq data of the purified nuclear Cajal body.
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[Journal Article] Thymus-specific noncoding RNA, Thy-ncR1, is a cytoplasmic riboregulator that facilitates hUPF1-dependent degradationof MFAP4 mRNA in specific T-cell leukemia cells.2010
Author(s)
Aoki, K., Harashima, A., Sano, M. Yokoi, T., Nakamura, S., Kibata, M. Hirose T.
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Journal Title
BMC Mol Biol. 11
Pages: 99
Peer Reviewed
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[Journal Article] Transcripts of unknown function in multiple-signaling pathways involved in human stem cell differentiation.2009
Author(s)
Kikuchi, K., Fukuda, M., Ito, T., Inoue, M., Yokoi, T., Chiku, S., Mitsuyama, T., Asai, K., Hirose T., Aizawa, Y.
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Journal Title
Nucleic Acid Res. 37
Pages: 4987-5000
Peer Reviewed
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