Analysis of the membrane stress response in E. coli

2010 Fiscal Year Final Research Report

• Project/Area Number: 20370078
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Kyoto University
• Principal Investigator: AKIYAMA Yoshinori 京都大学, ウイルス研究所, 教授 (10192460)
• Co-Investigator(Kenkyū-buntansha): MORI Hiroyuki 京都大学, ウイルス研究所, 准教授 (10243271)
• Project Period (FY): 2008 – 2010
• Keywords: 生体膜

Research Abstract

To elucidate the mechanism of activation of extracytoplasmic stress response by abnormal membrane proteins("membrane stress response),(1) we isolated and analyzed suppressor mutations against a class of the secY mutations that elicit membrane stress response, and(2) from the analysis of RseP PDZ domain mutants, we suggest a new Sigma E activation memsahib via ligand binding to the PDZ domains.

Research Products

• [Journal Article] Quality control of cytoplasmic membrane proteins in Escherichia coli (2009)
  • Author(s): Akiyama, Y.
  • Journal Title: J. Biochem. Volume: 46 Pages: 449-454
  • Peer Reviewed
• [Journal Article] The Sec protein secretion system (2009)
  • Author(s): Ito, K. and Mori, H.
  • Journal Title: Bacterial Secreted Proteins Pages: 3-22
• [Journal Article] Substrate recognition and binding by RseP, an Escherichia coli intramembrane protease (2008)
  • Author(s): Koide, K., Ito. K., and Akiyama, Y.
  • Journal Title: J. Biol. Chem. Volume: 283 Pages: 9562-9570
  • Peer Reviewed
• [Journal Article] Conformational transition of Sec machinery inferred from bacterial SecYE structures (2008)
  • Author(s): Tsukazaki, T., Mori, H., Fukai, S., Ishitani, R., Mori, T., Dohmae, N., Perederina, A., Sugita, Y., Vassylyev, D. G., Ito, K. andNureki O.
  • Journal Title: These authors contributed equally to thiswork Volume: 455 Pages: 988-991
  • Peer Reviewed
• [Journal Article] A pair of circularly permutated PDZ domains control RseP, the S2P family intramembrane protease of E. coli (2008)
  • Author(s): Inaba, K., Suzuki, M., Maegawa, K., Akiyama, S., Ito, K., and Akiyama, Y.
  • Journal Title: J. Biol. Chem. Volume: 283 Pages: 35042-35052
  • Peer Reviewed
• [Presentation] 大腸菌表層ストレス応答に関わるS2P膜内プロテアーゼRsePのPDZドメイン機能の解析 (2010)
  • Author(s): 檜作洋平、秋山芳展
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] Roles of the two PDZ domains of RseP, the S2P family intramembrane protease of Escherichia coli, in regulation of its protease function (2010)
  • Author(s): Hizukuri, Y. and Akiyama, Y.
  • Organizer: The 3rd International Symposium on Protein Community, Nara
  • Place of Presentation: Japan
  • Year and Date: 20100913-16
• [Presentation] New function of RseP, the E. coli S2P family protease (2010)
  • Author(s): Akiyama, Y.
  • Organizer: New function of RseP, the E. coli S2P family protease. Gordon Research Conference on Proteolytic enzymes & the irinhibitors
  • Place of Presentation: Lucca, Italy
  • Year and Date: 20100502-07
• [Presentation] A pair of circularly permutated PDZ domains control RsePRse, the S2P family intramembrane protease of E. coli. (2009)
  • Author(s): Inaba, K., Suzuki, M., Maegawa, K.-i., Akiyama, S., Ito, K. and Akiyama, Y.
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 20091021-24
• [Presentation] 大腸菌S2PプロテアーゼRsePの膜タンパク質品質管理における新たな役割 (2008)
  • Author(s): 斎藤啓、秋山芳展
  • Organizer: 第5回21世紀大腸菌研究会
  • Place of Presentation: 藤枝
  • Year and Date: 20080728-29
• [Presentation] 大腸菌RIPプロテアーゼの機能と制御.ワークショップ「直接性、不可逆性を特徴とする新しいシグナル伝達のメカニズム」 (2008)
  • Author(s): 秋山芳展
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-11
• [Remarks]
  • URL: http://www.virus.kyoto-u.ac.jp/Lab/akiyama/index.html